Nuclear morphometry in relation to tumor grade in canine spontaneous cutaneous squamous cell carcinomas

In a retrospective study on cytological specimens from 17 dogs with histologically confirmed cutaneous squamous cell carcinoma, the morphometric variables were studied and compared to tumors' nuclear grade. The morphometric parameters evaluated in this study were mean nuclear area (MNA, µm2), mean nuclear perimeter (MNP, µm), mean nuclear diameter (D mean, µm), minimum nuclear diameter (D min, µm), and maximum nuclear diameter (D max, µm). Associations between MNA, MNP, D mean, D min, and D max and tumors' grade were assessed using the ANOVA/LSD test (Statistica 6.0, StatSoft, USA) at a level of significance of P < 0.05. The result indicated an increase in the mean values of the nuclear parameters with increasing tumor grade. In the cases examined, significant differences in MNA, MNP, and D mean occurred between tumors in all grades. Besides, no significant differences in D max (between grade I-II and II-III) and D min (grade II-III) were found. In conclusion, nuclear morphometry could be used as an additional tool for differentiating nuclear grade in canine cutaneous squamous cell carcinomas.

Anahtar Kelimeler:

Key words: Image analysis, nuclear morphometry, tumor grade, canine cutaneous squamous cell carcinomas

Nuclear morphometry in relation to tumor grade in canine spontaneous cutaneous squamous cell carcinomas

Keywords:

Key words: Image analysis, nuclear morphometry, tumor grade, canine cutaneous squamous cell carcinomas,

PDF

___

Simeonov, R., Simeonova, G.: Computerized morphometry of mean nuclear diameter and nuclear roundness in canine mammary gland tumors on cytologic smears. Vet. Clin. Pathol., 2006; 35: 88-90.
Simeonov, R., Simeonova, G.: Fractal dimension of canine mammary gland epithelial tumors on cytologic smears. Vet. Clin. Pathol., 2006; 35: 446-448.
Simeonov, R., Simeonova, G.: Use of quantitative analysis as a method for differentiation between canine cutaneous apocrine adenomas and apocrine carcinomas on cytological smears. J. Vet. Med. A, Physiol. Pathol. Clin. Med., 2007; 54: 542-544.
Simeonov, R., Simeonova, G.: Computerized cytomorphometric analysis of nuclear area, nuclear perimeter and mean nuclear diameter in spontaneous canine mammary gland tumours. Vet. Res. Commun., 2007; 31: 553-558.
Simeonov, R., Simeonova, G.: Nuclear cytomorphometry in feline mammary gland epithelial tumours. Vet. J., 2009; 179: 296-300.
Simeonov, R., Simeonova, G.: Nucleomorphometric analysis of feline basal cell carcinomas. Res. Vet. Sci., 2008; 84: 440-443.
Goldschmidt, M.H., Dunstan, R.W., Stannard, A.A., Von Tscharner, C., Walder, E.J., Yager, J.A.: Histological classification of epithelial and melanocytic tumors of the skin of domestic animals. World Health Organization International Classification of Tumors in Domestic Animals. Second Series, Vol III. Washington D.C.: Armed Forces Institute of Pathology, American Registry of Pathology, 1998.
Goldschmidt, M.H., Shofer, F.S.: Skin Tumour of the Dog and Cat. Pergamon Press, Oxford. 1992; 16-49.