Öğünç MERAL, Serdar KAMANLI, Tansel ANSAL BALCI, Birkan KARSLI, Ahmet Nuri TAŞDEMİR, Zişan Servet YALÇIN, Bülent TARIM, Engin TÜLEK, Hüseyin AYGÖREN, Mehmet BOZKURT

Effect of eggshell temperatures on hatching performance, egg production, and bone morphology of laying hens

Abstract: The present study aims to evaluate the effect of lower or higher eggshell temperatures from days 7 to 21 on hatching performance, bone and blood parameters, and egg production along with the age of laying chickens. A total of 3150 eggs obtained from a white egg-type breeder were divided randomly into 3 groups of 1050 eggs. From 7 to 21 days, eggs were incubated at one of three eggshell temperatures: Control (IC) 37.5 °C, low (IL) 36.9 °C, and high (IH) 38.5 °C. At hatch, chicks were weighed, and blood was collected to measure total Ca, inorganic P, and ALP level weights, dimensions, and ash contents of femur, humerus, and tibia were obtained. A total of 240 chicks from each incubation temperature were reared up to 58 weeks. The results showed that hatchability was similar among the eggshell temperature groups. The IL chicks had the heaviest chick weight and yolk free chick weight. The IH group had reduced chick weight compared to IL and IC but increased lengths of tibia and humerus, ash contents of tibia and femur, and blood Ca levels at the day of the hatch. Laying hens from the IH group had impaired body weight during the laying cycle. At 58 weeks, there was no effect of eggshell temperature on egg production, bone length, and width. IH hens had lower tibia ash and serum Ca but higher P and ALP levels compared to IL and IC groups. However, bone mineral content and mineral density were similar for hens from different eggshell temperature groups. In conclusion, it appeared that although chicks from the IH group had reduced chick weight, they had improved bone morphology and ash content of bones compared to IC and IL at hatch. However, IH hens could not maintain the higher ash content during the grow-out and laying periods. The results indicate that the positive effect of IH incubation temperature on bone morphological measurements and ash content of tibia would not be long term.Key words: Incubation temperature, laying chick, laying hen, bone, egg production

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1. Yalçin S, Siegel PB. Exposure to cold or heat during incubation on developmental stability of broiler embryos. Poultry Science 2003; 82 (9): 1388-1392. doi: 10.1093/ps/82.9.1388
2. Hammond CL, Simbi BH, Stickland NC. In ovo temperature manipulation influences embryonic motility and growth of limb tissues in the chick (Gallus gallus). Journal of Experimental Biology 2007; 210 (15): 2667-2675. doi: 10.1242/ jeb.005751
3. Yalçin S, Molayoglu HB, Baka M, Genin O, Pines M. Effect of temperature during the incubation period on tibial growth plate chondrocyte differentiation and the incidence of tibial dyschondroplasia. Poultry Science 2007; 86 (8): 1772-1783. doi:10.1093/ps/86.8.1772
4. Van Der Pol CW, Van Roovert-Reijrink IAM, Maatjens CM, Van Den Anker I, Kemp B et al. Effect of eggshell temperature throughout incubation on broiler hatchling leg bone development. Poultry Science 2014; 93 (11): 2878-2883. doi:10.3382/ps.2014-04210
5. Oviedo-Rondón EO, Wineland MJ, Small J, Cutchin H, McElroy A et al. Effect of incubation temperatures and chick transportation conditions on bone development and leg health. Journal of Applied Poultry Research 2009; 18 (12): 671- 678. doi: 10.3382/japr.2008-00135
6. Güz BC, Molenaar R, de JongIC, Kemp B, vanKrimpen M, et al. Effects of egg shell temperature pattern during incubation on tibia characteristics of broiler chickens at slaughter age. Poultry Science 2020, 99 (6): 3020-3029. doi.org/10.1016/j. psj.2019.12.042
7. Sgavioli S, Santos ET, Domingues CHF, Quadros DMC, Andrade-Garcia GM et al. Effect of high incubation temperature on the blood parameters of layer chicks. Brazilian Journal of Poultry Science 2016; 18 (2): 45-52. doi: 10.1590/1806-9061- 2015-0095
8. Whitehead CC, Fleming RH. Osteoporosis in cage layers. Poultry Science 2000; 79 (7): 1033-1041. Doi:10.1093/ ps/79.7.1033
9. Gregory NG, Wilkins LJ. Broken bones in domestic fowl: handling and processing damage in end-of-lay battery hens. British PoultryScience1989; 30 (3): 555-562. doi: 10.1080/00071668908417179
10. Bishop SC, Fleming RH, McCormack HA, Flock DK, Whitehead CC. Inheritance of bone characteristics affecting osteoporosis in laying hens. British Poultry Science, 2000; 41 (1):33-40. doi: 10.1080/00071660086376
11. Leyendecker M, Hamann H, Hartung J, Kamphues J, Neumann U et al. Keeping laying hens in furnished cages and an aviary housing system enhances their bone stability. British Poultry Science 2005; 46 (5): 536-544.doi :10.1080/00071660500273094
12. Donaldson CJ, Ball MEE, O’Connell NE. Aerial perches and free-range laying hens: the effect of access to aerial perches and of individual bird parameters on keel bone injuries in commercial free-range laying hens. Poultry Science 2012; 91 (2): 304-315. doi: 10.3382/ps.2011-01774.
13. Bar A. Calcium transport in strongly calcifying laying birds: mechanisms and regulation. Comparative Biochemistry and Physiology - Part A: A Molecular and Integrative Physiology 2009; 152 (4): 447-469. doi: 10.1016/j.cbpa.2008.
14. Zhang B, Coon CN. The relationship of various tibia bone measurements in hens. Poultry Science 1997; 76 (12): 1698- 1701. doi: 10.1093/ps/76.12.1698
15. Robinson CI and Karcher D. Analytical bone calcium and bone ash from mature laying hens correlates to bone mineral content calculated from quantitative computed tomography scans. Poultry Science 2019; 98 (9): 3611-3616. doi:10.3382/ps/ pez165
16. Rath NC, Huff GR, Huff WE, Balog JM. Factors regulating bone maturity and strength in poultry. Poultry Science 2000; 79 (7): 1024-1032. Doi:10.1093/ps/79.7.1024
17. Almeida Paz ICL, Bruno LDG. Bone mineral density: review. Brazilian Journal of Poultry Science 2006; 8(2): 69-73. Doi:10.1590/S1516-635X2006000200001
18. Tilgar V, Kilgas P, Viitak A, Reynolds SJ. The rate of bone mineralization in birds directly related to alkaline phosphatase activity. Physiological and Biochemical Zoology 2008; 81 (1): 106-111. doi: 10.1086/523305
19. Li C, Geng F, Huang X, Ma M, Zhang X. Phosvitin phosphorus is involved in chicken embryo bone formation through dephosphorylation. Poultry Science 2014; 93 (12): 3065-3072. doi: 10.3382/ps.2014-04098
20. SAS 2000. JMP Statistics and Graphics Guide, version 5.0 (Cary, NC, SAS Institute Inc.).
21. Fasenko GM, Wilson JL, Robinson FE, Hardin RT. Effects of length of egg nest holding time and high environmental temperatures on prestorage embryonic development, survival, and hatch ability of broiler breeders. Journal of Applied Poultry Research 1999; 8 (4): 488-492. doi:10.1093/japr/8.4.488
22. Decuypere E, Michels H. Incubatio ntemperature as a management tool: A review. World’s Poultry Science Journal 1992; 48: 28-38. doi:10.1079/WPS19920004
23. French NA. The critical importance of incubation temperature. Avian Biology Research 2009; 2 (1/2): 55-59. Doi:10.3184/175815509X431812
24. Joseph NS, Lourens A, Moran ET. The effects of suboptimal eggshell temperature during incubation on broiler chick quality, live performance, and further processing yield. Poultry Science 2006; 85: 932-938. doi: 10.1093/ps/85.5.932
25. Hulet R, Gladys G, Hill D, Meijerhof R, El-Shiekh T. Influence of egg shell embryonic incubation temperature and broiler breeder flock age on posthatch growth performance and carcass characteristics. Poultry Science 2007; 86 (5): 408-412. doi: 10.1093/ps/85.5.932.
26. Molenaar R, Reijink IAM, Meijerhof R, Van den Brand H. Meeting embryonic requirements of broilers throughout incubation: a review. The Brazilian Journal of Veterinary Research and Animal Science 2010; 12 (3): 137-148. doi:10.1590/S1516- 635X2010000300001
27. Sgavioli S, Santos ET, Domingues CHF, Quandros TCO, Castiblanco DMC. et al. Effect of high incubation temperature on the blood parameters of layer chicks. Brazilian Journal of Poultry Science, 2016; 18 (2): 41-47. Doi:10.1590/1806-9061- 2015-0095
28. Yalçin S, Cabuk M, Bruggeman V, Babacanoglu E, Buyse J, Decuypere E, Siegel PB. Acclimation to heat during incubation. 1. Embryonic morphological traits, blood biochemistry, and hatching performance. Poultry Science 2008; 87 (6): 1219- 1228.Doi:10.3382/ps.2007-00435
29. Kamanli S, Durmuş I, Yalcin S, Yıldırım U, Meral Ö. Effect of prenatal temperature conditioning of laying hen embryos: Hatching, live performance and response to heat and cold stress during laying period. Journal of Thermal Biology 2015; 51 (4): 96-104.doi: 10.1016/j.jtherbio.2015.04.001
30. Molenaar R, deVries S, van der Anker I, Meijerhof R, Kemp B. et al. Effect of eggshell temperature and a hole in the air cell on the perinatal development and physiology of layer hatchlings. Poultry Science 2010; 89 (8): 1716-1723. doi: 10.3382/ps.2010- 00779
31. Wineland MJ, Mann KM, Fairchild BD, Christensen VL. Effect of high and low incubator temperatures at different stages of incubation upon the broiler embryo. Poultry Science 2000; 79: S180.
32. Shim M, Karnuah YAB, Mitchell AD, Anthony NB, Pesti GM et al. The effects of growth rate on leg morphology and tibia breaking strength, mineral density, mineral content, and bone ash in broilers. Poultry Science 2012; 91 (8): 1790-1795.doi: 10.3382/ps.2011-01968.
33. Mackie EJ, Ahmed YA, Tararczuch L, Chen KS, Mirams M. Endochondral ossification: How cartilage is converted into bone in the developing skeleton. The International Journal of Biochemistry & Cell Biology 2008; 40 (1): 46-62.doi:10.1016/j. biocel.2007.06.009
34. Van Der Eerden BCJ, Karperien M, Wit JM. Systemic and local regulation of the growth plate. Endocrine Review 2003; 24 (6): 782-801. doi: 10.1210/er.2002-0033.
35. Bassett JHD, Graham RW. Role of thyroid hormones in skeletal development and bone maintenance. Endocrine Review 2016; 37 (2): 135-187. doi:10.1210 /er.2015-1106