Dharmendra CHHARANG, Sheela CHOUDHARY, Lenin BHATT

Assessment of blood metabolites, serum enzymes, and serum minerals in dietary probiotics fed captive Asian elephants

Abstract: This study was organised to evaluate the effect of supplementation of dietary probiotics on blood biochemistry in 18 captive Asian elephants of 30-62 years age and 3495 ± 133.34 kg body weight (BW) for two months. To observe the results in different groups, i.e. control with no probiotic (T1) and the other two with probiotics which contained 1 × 109CFU/g concentrate of Lactobacillus acidophilus (T2) and 1 × 109CFU/g concentrate of Saccharomyces cerevisiae (T3), they were given 50 kg BW/day (dose range 48-89 g per day/elephant). The elephants were randomly divided into three groups with six elephants each. They were fed on ad libitum green fodder. Blood biochemical constituents namely: (i) blood metabolites like glucose, total protein, albumin, cholesterol, creatinine, and blood urea nitrogen, (ii) serum enzymes like aspartate transaminase, alanine transaminase, and alkaline phosphatase, and (iii) serum minerals like calcium and phosphorus were analysed at the end of research to evaluate the physiological impacts of nutrients in the elephant’s body. It didn’t establish any health problem to the elephants since the parameters determined in the blood were observed well within the normal physiological range, and no significant differences were noticed due to the effect of treatment. It is concluded that blood biochemistry remained unaffected on the supplementation of probiotics in Asian elephants.Key words: Asian elephant, blood biochemistry, physiological impact, probiotics

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1. Clauss M, Loehlein W, Kienzle E, Wiesner H. Studies on feed digestibilities in captive Asian elephants (Elephas maximus). Journal of Animal Physiology and Animal Nutrition 2003; 87: 160-173.
2. Clements ET, Maloiy GMO. The digestive physiology of three East African herbivores: the elephant, rhinoceros and hippopotamus. Journal of Zoology (London) 1982; 198: 141-156.
3. Alayande KA, Aiyegoro OA, Ateba CN. Probiotics in animal husbandry: applicability and associated risk factors. Sustainability 2020; 12: 1087. doi:10.3390/su12031087
4. Zhao P, Kim I. Effect of direct-fed microbial on growth performance, nutrient digestibility, fecal noxious gas emission, fecal microbial flora and diarrhea score in weanling pigs. Animal Feed Science and Technology 2015; 200: 86-92.
5. Lan R, Tran H, Kim I. Effects of probiotic supplementation in different nutrient density diets on growth performance, nutrient digestibility, blood profiles, fecal microflora and noxious gas emission in weaning pig. Journal of the Science of Food and Agriculture 2017; 97: 1335-1341.
6. Pambu-Gollah R, Cronje PB, Casey NH. An evaluation of the use of blood metabolite concentrations as indicators of nutritional status in free-ranging indigenous goats. South African Journal of Animal Science 2000; 30: 115-120.
7. Agenas S, Heath MF, Nixon RM, Wilkinson JM, Phillips CC. Indicators of under-nutrition in animal. Animal Welfare 2006; 15: 149-160.
8. Lindsay DB, Hunter RA, Gazzola C, Spiers WG, Sillence MN. Energy and growth. Australian Journal of Agricultural Research 1993; 44: 875-889.
9. Wettemann RP, Lents CA, Ciccioli NH, White FJ, Rubio I. Nutritional and suckling mediated an ovulation in beef cows. Journal of Animal Science 2003; 81 (2): 48-59.
10. Ndlovu T, Chimonyo M, Okoh AI, Muchenje V, Dzama K et al. Assessing the nutritional status of beef cattle: current practices and future prospects. African Journal of Biotechnology 2007; 6 (24): 2727-2734.
11. Olorode BR, Longe OG. Growth, nutrient retention, haematology and serum chemistry of pullet chicks fed shea butter cake in the humid tropics. Archivos De Zootecnia 1999; 49 (187): 444.
12. Steel RGD, Torrie JH, Dickey DA. Principles and Procedures of Statistics. A Biometrical Approach. 3rd ed. New York, USA: McGraw Hill Book Company; 1997.
13. Niemuller C, Gentry PA, Liptrap RM. Longitudinal study of haematological and biochemical constituents in blood of the Asian elephant (Elephas maximus). Comparative Biochemistry and Physiology 1990; 96A (1): 131-134.
14. Das A, Saini M, Katole S, Kullu SS, Swarup D et al. Effect of feeding different levels of wheat roti on nutrient utilization and blood metabolite profile in semi-captive Asian elephants (Elephas maximus). Journal of Animal Physiology and Animal Nutrition 2015; 99: 367-378.
15. Sahoo M. Management of foot lesions in Asian elephants (Elephas maximus) of Odisha. M.V.Sc. Orissa University of Agriculture and Technology, Odisha, 2015.
16. Simonová M, Marcinakova M, Strompfova V, Cobanova K, Gancarcikova S et al. Effect of probiotics Lactobacillus rhamnosus gg and new isolate Enterococcus faecium ef2019 (ccm 7420) on growth, blood parameters, microbiota and coccidia oocysts excretion in rabbits. International Journal of Probiotics and Prebiotics 2008; 3 (1): 7-14.
17. Seyidoğlu N, Galip N. Effects of Saccharomyces cerevisiae and Spirulina platensis on growth performances and biochemical parameters in rabbits. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2014; 20: 331-336. doi:10.9775/kvfd.2013.9988
18. Sarat Chandra A, Mahender M, Harikrishna C. Effect of dietary supplementation of probiotics and enzymes on the blood biochemistry and immune competence of rabbits reared under two housing systems. Livestock Research International 2015; 3 (1): 20-24.
19. Belhassen T, Bonai A, Gerencsér Zs, Matics Zs, Tuboly T et al. Effect of diet supplementation with live yeast Saccharomyces cerevisiae on growth performance, caecal ecosystem and health of growing rabbits. World Rabbit Science 2016; 24: 191- 200. doi:10.4995/wrs.2016.3991
20. Kalma RP, Chauhan HD, Srivastava AK, Pawar MM. Growth and blood profile of broiler rabbits on probiotic supplementation. Indian Journal of Small Ruminants 2018; 24 (1): 66-69. doi: 10.5958/0973-9718.2018.00031.4
21. Kumar S, Verma AK, Mondal SK, Gupta M, Patil A et al. Effect of live Saccharomyces cerevisiae feeding on serum biochemistry in early weaned cross bred piglets. Veterinary World 2012; 5 (11): 663-666.
22. Katole S, Das A, Agarwal N, Prakash B, Saha SK et al. Influence of work on nutrient utilisation in semicaptive Asian elephants (Elephas maximus). Journal of Applied Animal Research 2014; 42 (4): 380-388. doi: 10.1080/09712119.2013.875904
23. Janyamethakul T, Sripiboon S, Somgird C, Pongsopawijit P, Panyapornwithaya V et al. Hematologic and biochemical reference intervals for captive Asian elephants (Elephas maximus) in Thailand. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2017; 23 (4): 665-669. doi: 10.9775/kvfd.2017.17380
24. Antunovic Z, Speranda M, Liker B, Seric V, Sencic D et al. Influence of feeding the probiotic Pioneer PDFM to growing lambs on performances and blood composition. Acta Veterinaria-Beograd 2005; 55: 287-300.
25. Onifade AA, Obiyan RI, Onipede E, Adejumo DO, Abu OA et al. Assessment of the effects of supplementing rabbit diet with a culture of “Saccharomyces cerevisiae” using growth performance, blood composition and clinical enzyme activities. Animal Feed Science and Technology 1999; 77: 25- 32. doi:10.1016/S0377-8401(98)00244-2
26. Dong X, Zhang N, Zhou M, Tu Y, Deng K et al. Effects of dietary probiotics on growth performance, faecal microbiota and serum profiles in weaned piglets. Journal of Basic Microbiology 2013; 49 (2): 220-226.
27. Dowarah R, Verma AK, Agarwal N, Singh P, Singh BR. Selection and characterization of probiotic lactic acid bacteria and its impact on growth, nutrient digestibility, health and antioxidant status in weaned piglets. PLoS ONE 2018; 13: e0192978.
28. El-Sayed AA, Mousa SA. Effects of administration of probiotic on body growth and hematobiochemical profile in growing Barki lambs. Comparative Clinical Pathology 2019; 29: 297- 303.
29. Allwin B, Senthil KG, Kalaignan Pa, Senthil NR, Vairamuthu S et al. Serum biochemistry of the Asian elephant. International Journal of Advanced Multidisciplinary Research 2015; 2 (4): 62-66.
30. Kwan OS, Kim IH, Hong W, Han YK, Lee SH et al. Effects of probiotics supplementation on growth performance, blood composition, and fecal noxious gas of broiler chickens. Korean Journal of Poultry Science 2002; 1: 1-6.
31. Mustari A, Ahmad N. Effects of probiotics on serum biochemical parameters in rats. The Bangladesh Veterinarian 2011; 28 (2): 70-74.
32. Salahuddin M, Akhter H, Akter S, Miah MA, Ahmad N. Effects of probiotics on haematology and biochemical parameters in mice. The Bangladesh Veterinarian 2013; 30 (1): 20-24.
33. El-deek AA, Albanoby MA, El-Naga MKA. Evaluation of super action probiotic as a natural growth promoter for growing rabbits. Egyptian Poultry Science Journal 2013; 33: 407-419.
34. McCain S, Allender MC, Schumacher J, Ramsay E. The effects of a probiotic on blood urea nitrogen and creatinine concentrations in large felids. Journal of Zoo and Wildlife Medicine 2011; 42 (3): 426-429. doi: http://dx.doi. org/10.1638/2010-0235.1
35. Wang F, Jiang YS, Liu F. The influence of mutant lactobacilli on serum creatinine and urea nitrogen concentrations and renal pathology in 5/6 nephrectomized rats. Renal Failure 2016; 38 (9): 1441-1447. doi: 10.1080/0886022X.2016.1227617
36. Mašek T, Mikulec Z, Valpotic H, Antunac N, Mikulec N et al. Influence of live yeast culture (Saccharomyces cerevisiae) on milk production and composition, and blood biochemical of grazing dairy ewes during the milking period. Acta Veterinaria Brno 2008; 77: 547-554.
37. Quezada T, Cuellar H, Valdiviaand AG, Reys JI. Effects of aflatoxin B1 on the liver and kidneys of broiler chickens during development. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2002; 125: 265-272.
38. Fatemi F, Allameh A, Dadkhah A, Forouzadeh M, Kazemnejad S et al. Changes in hepatic cytosolic glutathione S-transferase activity and expression of its class-P during prenatal and postnatal periods in rats treated with aflatoxin B1. Archives of Toxicology 2006; 80: 572-579.
39. Ozyurt B, Iraz M, Koca K, Ozyurt H, Sahin S. Protective effects of caffeic acid phenethyl ester on skeletal muscle ischemiareperfusion injury in rats. Molecular Cell Biochemistry 2006; 292: 197-203.
40. Scholl PF, McCoy L, Kensler TW, Groopman GD. Quantitative analysis and chronic dosimetry of the aflatoxin B1 plasma albumin adduct Lys-AFB1 in rats by isotope dilution mass spectrometry. Chemical Research in Toxicology 2006; 19: 44- 49.
41. Cenesiz S, Yaman H, Ozcan A, Kart A, Karademir G. Effects of Kefir as a probiotic on serum cholesterol, total lipid, aspartate amino transferase and alanine aminotransferase activities in broiler chicks. Medycyna Weterynaryjna 2008; 64: 168-170.
42. Vahdatpour T, Babazadeh D. The effects of kefir rich in probiotic administration on serum enzymes and performance in male Japanese quails. Journal of Animal and Plant Sciences 2016; 26: 34-39.
43. Shrivastava AK, Jha RR. Effects of different chemical composition and probiotic on the haematobiochemical profile of rabbit. Electronic Journal of Environmental, Agricultural and Food Chemistry 2010; 9: 1507-13.
44. Brown IRF, White PT. Elephant blood haematology and chemistry. Comparative Biochemistry and Physiology 1980; 65B: 1-12.
45. Gromadzka-Ostrowska J, Jakubów K, Zalewska B, Krzywicki Z. Haematological and blood biochemical studies in female domesticated Indian elephants (Elaphas maximus L.). Comparative Biochemistry and Physiology Part A: Physiology 1988; 89 (3): 313-315. doi:10.1016/0300-9629(88)91031-6
46. Kunavue N, Lien T. Effects of fulvic acid and probiotic on growth performance, nutrient digestibility, blood parameters and immunity of pigs. Journal of Animal Science Advances 2012; 2 (8): 711-721.
47. Hashemzadeh F, Rahimi S, Torshizi MA, Masoudi AA. Effects of probiotics and antibiotic supplementation on serum biochemistry and intestinal microflora in broiler chicks. International Journal of Agriculture and Crop Sciences 2013; 5 (20): 2394.
48. Soleymanzadeh MS, FathiZadeh S, Majidpour A, Mohammad N, Fagheei AZ et al. Have probiotic therapy effect on calcium and cholesterol serum levels in demyelinated hippocampus? Infection, Epidemiology and Microbiology 2017; 3 (3): 82-85.
49. Simonová MP, Lauková A, Chrastinova L, Stpová V, Faix S et al. Enterococcus faecium CCM7420, bacteriocin PPB CCM7420 and their effect in the digestive tract of rabbits. Czech Journal of Animal Science 2009; 54: 376-386.
50. Shah AA, Yuan X, Khan RU, Shao T. Effect of lactic acid bacteria-treated King grass silage on the performance traits and serum metabolites in New Zealand white rabbits (Oryctolagus cuniculus). Journal of Animal Physiology and Animal Nutrition 2017; 00: 1-7. https://doi.org/10.1111/jpn.12854
51. Jatkauskas J, Vrotniakienė V. Effect of L-plantarum, Pediococcus acidilactici, Enterococcus faecium and L-lactis microbial supplementation of grass silage on the fermentation characteristics in rumen of dairy cows. Veterinarija Ir Zootechnika 2007; 1: 40-62.