Özgür ÖZDEMİR, Yasemin PINARKARA, Zafer SAYIN, Osman ERGANİŞ, Aslı SAKMANOĞLU, Hasan Hüseyin HADİMLİ

6995

Effcacy of experimental inactivated and live Rhodococcus equi vaccines for thoroughbred Arabian mares in mice

Effcacy of experimental inactivated and live Rhodococcus equi vaccines for thoroughbred Arabian mares in mice

The aim of this study was to determine the effcacy of inactive Rhodococcus equi vaccine candidates included thatbacterin+aluminum hydroxide (Al(OH)3), bacterin+VapA+(Al(OH)3), bacterin+Montanide IMS 3012 (IMS), bacterin+VapA+IMS,and live vaccine using mice as a model. Te efcacy of vaccine was evaluated according to clinical fndings, humoral and cellularimmunity (levels of INF-g and IL-4), and results of microbiological culture from internal organs in dead or sacrifced mice. Inactive R.equi vaccines were subcutaneously administered to mice three times at 15-day intervals and live vaccine was intraperitoneally injectedonce. Fifeen days afer the last vaccination, aerosol challenges were carried out with the pathogenic R. equi VapA+K2002 strain in allgroups. Two mice were sacrifced from each challenge groups on days 1, 3, 5, and 7. The antibody titers of vaccinated mice were found tobe signifcantly higher than those of the controls. The largest number of INF-g positive samples were detected in the bacterin+VapA+IMSand bacterin+IMS groups. IL-4 positivity was determined only in live vaccine groups. The lowest reisolation rate of R. equi from internalorgans was observed in the bacterin+VapA+IMS group. It was concluded that R. equi vaccines, and especially bacterin+VapA+IMS, areuseful to protect mice against R. equi infection.
PDF

___

  • 1. Meijer WG, Prescott JF. Rhodococcus equi. Vet Res 2004; 35: 383–396.
  • 2. Woolcock JB, Mutimer MD, Farmer AMT. Epidemiology of Corynebacterium equi in horses. Res Vet Sci 1980; 28: 87–90.
  • 3. Muscatello G. Rhodococcus equi pneumonia in the foal. Part 1: Pathogenesis and epidemiology. Vet J 2012; 192: 20–26.
  • 4. Takai S, Sekizaki T, Ozawa T, Sugawara T, Watanabe Y, Tsubaki S. Association between a large plasmid and 15- to 17-kilodalton antigens in virulent R. equi . Infect Immun 1991; 59: 4056–4060.
  • 5. Pei Y, Nicholson V, Woods C, Prescott CF. Immunization by intrabronchial administration to 1-week-old foals of an unmarked double gene disruption strain of Rhodococcus equi strain 103+. Vet Microbiol 2007; 125: 100–110.
  • 6. Cohen ND, Smith KE, Ficht TA, Takai S, Libal MC, Becu T, Leadon DP, Chafn MK, Martens RJ. Genetic variability among isolates of Rhodococcus equi obtained from horses and horse farms: Clinical implications. In: 49th Annual Convention of American Association of Equine Practitioners; New Orleans, LA, USA; 2003. pp. 385–390.
  • 7. Ainsworth DM, Eicker SW, Yeagar AE, Sweeney CR, Viel L, Tesarowski D, Lavoie JP, Hofman A, Paradis MR, Reed SM et al. Associations between physical examination, laboratory, and radiographic fndings and outcome and subsequent racing performance of foals with Rhodococcus equi infection: 115 cases (1984-1992). J Am Med Assoc 1998; 213: 510–515.
  • 8. Hurley JR, Begg AP. Failure of hyperimmune plasma to prevent pneumonia caused by Rhodococcus equi in foals. Aust Vet J 1995; 72: 418–420.
  • 9. Becu T, Polledo G, Gaskin JM. Immunoprophylaxis of Rhodococcus equi pneumonia in foals. Vet Microbiol 1997; 56: 193–204.
  • 10. Butler JE. Immunoglobulin diversity, B-cell and antibody repertoire development in large farm animals. OIE Revue Scientifque et Technique 1998; 17: 43–70.
  • 11. Güler L, Gündüz K, Erganiş O, Durmaz M, Ok U, Gülcü Y. Rhodococcus equi pneumonia in foals: immunoprophylaxis, molecular and sero diagnostic studies. In: VI. National Congress of Veterinary Microbiology; Elazığ, Turkey; 2004 (in Turkish).
  • 12. Aguilar JC, Rodriguez EG. Vaccine adjuvants revisited. Vaccine 2007; 25: 3752–3762.
  • 13. Taouji S, Nomura I, Giguere S, Tomomitsu S, Kakuda T, Ganne V, Takai S. Immunogenicity of synthetic peptides representing linear B-cell epitopes of vapA of Rhodococcus equi. Vaccine 2004; 22: 1114–1123.
  • 14. Cauchard J, Sevine C, Ballet JJ, Taouji S. Foal IgG and opsonizing anti-Rhodococcus equi antibodies afer immunization of pregnant mares with a protective VapA candidate vaccine. Vet Microbiol 2004; 104: 73–81.
  • 15. Erganis O, Sayin Z, Hadimli HH, Sakmanoglu A, Pinarkara Y, Ozdemir O, Maden M. Te efectiveness of anti-R. equi hyperimmune plasma against R. equi challenge in thoroughbred Arabian foals of mares vaccinated with R. equi vaccine. Sci World J 2014; 2014: 480732.
  • 16. Phumoonna T, Barton MD, Vanniasinkam T, Heuzenroeder MW. Chimeric vap A/gro EL2DNA vaccines enhance clearance of Rhodococcus equi in aerosol challenged C3H/He mice. Vaccine 2008; 26: 2457–2465.
  • 17. Takai S, Kawazu S, Tsubaki S. Enzyme-linked immunosorbent assay for diagnosis of Corynebacterium (Rhodococcus) equi infection in foal. Am J Vet Res 1985; 46: 2166–2170.
  • 18. Prescott JF, Patterson MC, Nicholson VM, Morein B, Yager JA. Assessment of the immunogenic potential of Rhodococcus equi virulence associated protein (VapA) in mice. Vet Microbiol 1997; 56: 213–225.
  • 19. Whitehead AE, Parreira VR, Hewson J, Watson JL, Prescott JF. Development of a live, attenuated, potential vaccine strain of Rhodococcus equi expressing vapA and the virR operon and virulence assesment in the mouse. Vet Immun Immunpathol 2012; 145: 479–484.
  • 20. Vanniasinkam T, Barton MD, Heuzenroeder MW. Immune response to vaccines based upon the VapA protein of the horse pathogen, Rhodococcus equi, in a murine model. Int J Med Microbiol 2005; 294: 437–445.
  • 21. Hines SA, Kanaly ST, Byrne BA. Immunity to Rhodococcus equi. Vet Microbiol 1997; 56: 177–185.
  • 22. Oliveira AF, Soares SG, Roque-Barreira MC. Mice vaccination with VapA: challenge with Rhodococcus equi is followed by production of TH1 cytokines. Vet Immun Immunpathol 2009; 128: 241–242.
  • 23. Oliveira AF, Luciana PR, Cardoso SA, Soares SG, Roque- Barreira MC. Vaccination of mice with salmonella expressing VapA: mucosal and systemic T1 responses provide protection against Rhodococcus equi infection. PLoS One 2010; 5: e8644.
  • 24. Haghighi HR, Prescott JF. Assessment in mice of vapA–DNA vaccination against Rhodococcus equi infection. Vet Immunol Immunopathol 2005; 104: 215–225.
  • 25. Lopez AM, Townsand HG, Allen AL, Hondalus MK. Safety and immunogenicity of a live-attenuated auxotrophic candidate vaccine against the intracellular pathogen. Vaccine 2008; 26: 998–1009.
  • 26. Gonzalez-Iglesias P, Scortti M, MacArthur I, Hapeshi A, Rodriguez H, Prescott JF, Vazquez-Boland JA. Mouse lung infection model to assess Rhodococcus equi virulence and vaccine protection. Vet Microbiol 2014; 172: 256–264.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: 6
  • Yayıncı: TÜBİTAK
Arşiv
Sayıdaki Diğer Makaleler

Comparative effects of sirolimus-eluting versus bare metal stents on neointimal hyperplasia in balloon-injured external iliac artery of rabbits

Atul PATEL, Pinesh PARIKH, Deepak PATIL, Anila MATHEW, Suhas LELE, Mohan RAJAPURKAR, Pradip PATIL, Akhilesh KUMAR, Priti VANI

Effects of high-level chromium methionine supplementation in lambs fed a corn-based diet on the carcass characteristics and chemical composition of the longissimus muscle

Berenice SANCHEZ-MENDOZA, Antonio AGUILAR-HERNANDEZ, Maria A. LOPEZ-SOTO, Alberto BARRERAS, Alfredo ESTRADA-ANGULO, Francisco Javier Monge NAVARRO, Noemi G. TORRENTERA, Richard A. ZINN, Alejandro PLASCENCIA

Efficacy of experimental inactivated and live Rhodococcus equivaccines for thoroughbred Arabian mares in mice

OSMAN ERGANİŞ, HASAN HÜSEYİN HADİMLİ, ZAFER SAYIN, ASLI SAKMANOĞLU, YASEMİN PINARKARA, ÖZGÜR ÖZDEMİR

Y chromosome haplotype characterization of Tunisian sheep breeds

Samia KDIDI, MOHAMED HABIB YAHYAOUI, BELEN GARCÍA-MANRIQUE, Pilar SARTO, Mohsen Ben SASSI, Touhami KHORCHANI, JORGE HUGO CALVO

Novel coproducts from corn milling and their use in ruminants' nutrition

Catalin DRAGOMIR, Marketta RINNE, David YANEZ RUIZ

The assembly of virus-like particles of porcine transmissible gastroenteritisvirus in vitro by baculovirus expression system

SONG ZHENHUI, HAIBO FENG, ZHI ZHU, XIANJIN DAI, YUE ZHOU, YUNTIAN LI, XINZHI CAO

Effects of fermented cottonseed meal on the growth performance, apparent digestibility, carcass traits, and meat composition in yellow-feathered broilers

Cunxi NIE, Wenju ZHANG, Wenxia GE, Yongqiang WANG, Yangfeng LIU, Jiancheng LIU

Textural acceptability of prepared fish sausages by controlling textural indicators

Mehmet Tolga DİNÇER, Şükran ÇAKLI

The effect of macromolecule and growth factor combinations on in vitrodevelopment of bovine embryos

ALPER KOÇYİĞİT, MESUT ÇEVİK, UĞUR ŞEN, MEHMET KURAN

Novel coproducts from corn milling and their use in ruminants? nutrition

CATALIN DRAGOMIR, MARKETTA RINNE, DAVID YANEZ-RUIZ