ALPER KOÇYİĞİT, MESUT ÇEVİK, UĞUR ŞEN, MEHMET KURAN

The effect of macromolecule and growth factor combinations on in vitrodevelopment of bovine embryos

This study was conducted to determine the effects of different macromolecule sources added to synthetic oviduct fluid (SOF) culture medium supplemented with growth factors on the development of bovine embryos and blastocyst morphology. Zygotes were distributed into 5 treatment groups. Cleavage, morula, and blastocyst rates were evaluated under a stereomicroscope. Trophectoderm (TE) and inner cell mass (ICM) cells were determined by differential staining method. It was found that bovine serum albumin (BSA), either alone or in combination with growth factors, as compared to the control or polyvinyl-alcohol (PVA) resulted in higher embryo yield and faster development during early bovine embryo culture. The quality of bovine embryos, based on the number of blastocyst cells and the ratio of ICM to total blastomeres, was affected by the sources of macromolecules and their combinations with growth factors. Growth factors supplemented to SOFaa media with BSA and PVA significantly increased the number of ICM cells and the ratio of ICM cells to total number of cells. In conclusion, replacing BSA with PVA depressed the blastocyst rate and cell numbers, and the number of blastomeres and ICM and TE cell numbers were affected by both the type of macromolecule and the growth factor supplements.

Anahtar Kelimeler:

Bovine, embryo culture, blastocyst quality, macromolecules, growth factors

The effect of macromolecule and growth factor combinations on in vitrodevelopment of bovine embryos

Keywords:

Bovine, embryo culture, blastocyst quality, macromolecules, growth factors,

PDF

___

Ali A, Sirard MA. Effect of the absence or presence of various protein supplements on further development of bovine oocytes during in vitro maturation. Biol Reprod 2002; 66: 901–905.
Srisathien S, Hernandez-Fonseca HJ, Brackett BG. Influences of epidermal growth and insulin-like growth factor-I on bovine blastocyst development in vitro. Anim Reprod Sci 2003; 77: 21–32.
Rizos D, Gutierrez-Adan A, Perez-Garnelo S, de la Fuente J, Boland MP, Lonergan P. Bovine embryo culture in the presence or absence of serum: Implications for blastocyst development, cryotolerance, and messenger RNA expression. Biol Reprod 2003; 68: 236–243.
Duque P, Hidalgo CO, Gómez E, Pintado B, Facal N, Díez C. Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and quality. Reprod Nutr Dev 2003; 43: 487–496.
Kim JY, Kim SB, Park MC, Park H, Park YS, Park HD, Lee JH, Kim JM. Addition of macromolecules to PZM-3 culture medium on the development and hatching of in vitro porcine embryos. Asian-Aust J Anim Sci 2007; 20: 1820–1826.
Krisher RL, Lane M, Bavister BD. Developmental competence and metabolism of bovine embryos cultured in semi-defined and defined culture media. Biol Reprod 1999; 60: 1345–1352.
Palasz AT, Thundathil J, Verrall RE, Mapletoft RJ. The effect of macromolecular supplementation on the surface tension of TCM-199 and the utilization of growth factors by bovine oocytes and embryos in culture. Anim Reprod Sci 2000; 58: 229–240.
Mingoti GZ, Castro VS, Méo SC, Sá Barretto LS, Garcia JM. The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development. In Vitro Cell Dev Biol Anim 2001; 47: 361–367.
Shirazi A, Ardali MA, Ahmadi E, Nazari H, Mamuee M, Heidari B. The effect of macromolecule source and type of media during in vitro maturation of sheep oocytes on subsequent embryo development. J Reprod Infertil 2012; 13: 13–19.
Quetglas MD, Coelho LA, Garcia JM, Oliveira FEB, Esper CR. Effect of insulin-like growth factor-I during in vitro oocyte maturation and in vitro culture of bovine embryos. Arq Bras Med Vet Zootec 2001; 53: 1–5.
Van Soom A, Boerjan M, Ysebaert MT, Kruif A. Cell allocation to the inner cell mass and the trophectoderm in bovine embryos cultured in two different media. Mol Reprod Dev 1996; 45: 171–182.
Koo DB, Kang YK, Choi YH, Park JS, Kim HN, Oh KB, Son DS, Park H, Lee KK, Han YM. Aberrant allocations of inner cell mass and trophectoderm cells in bovine nuclear transfer blastocysts. Biol Reprod 2002; 67: 487–492.
Parrish JJ, Krogenaes A, Susko-Parrish JL. Effects of bovine sperm by either swim-up or Percoll method on success of in vitro fertilization and early embryonic development. Theriogenology 1995; 44: 859–869.
SAS. SAS User Guide Ver. 9.01. Cary, NC, USA: SAS; 2007.
Kuran M, Robinson JJ, Staines ME, McEvoy TG. Development and de novo protein synthetic activity of bovine embryos produced in vitro in different culture systems. Theriogenology 2001; 55: 593–606.
Rieger D, Luciano AM, Modina S, Pocar P, Lauria A, Gandolfi F. The effect of epidermal growth factor and insulin-like growth factor I on the metabolic activity, nuclear maturation and subsequent development of cattle oocytes in vitro. J Reprod Fertil 1998; 112: 123–130.
Diaz-Cueto L, Gerton GL. The influence of growth factors on the development of preimplantation mammalian embryos. Arch Med Res 2001; 32: 619–626.
Makarevich AV, Markkula M. Apoptosis and cell proliferation potential of bovine embryos stimulated with insulin-like growth factor I during in vitro maturation and culture. Biol Reprod 2002; 66: 386–392.
Herrler A, Lucas-Hahn A, Niemann H. Effects of insulin-like growth factor-I on in vitro production of bovine embryos. Theriogenology 1992; 37: 1213–1224.
Kurzawa R, Glabowski W, Baczkowski T, Wiszniewska B, Marchlewicz M. Growth factors protect in vitro cultured embryos from the consequences of oxidative stress. Zygote 2004; 12: 231–240.
Neira JA, Tainturier D, Pena MA, Martal J. Effect of the association of IGF-I, IGF-II, bFGF, TGF-β1, GM-CSF, and LIF on the development of bovine embryos produced in vitro. Theriogenology 2010; 73: 594–604.
Stojkovic M, Büttner M, Zakhartchenko V, Brem G, Wolf E. A reliable procedure for differential staining of in vitro produced bovine blastocysts: comparison of tissue culture medium 199 and Menezo’s B2 medium. Anim Reprod Sci 1998; 50: 1–9.
Van Soom A, Ysebaert MT, Kruif AD. Relationship between timing of development, morula morphology, and cell allocation to inner cell mass and trophectoderm in in vitro produced bovine embryos. Mol Reprod Dev 1997; 47: 47–56.
Kuran M, Robinson JJ, Brown DS, McEvoy TG. Development, amino acid utilization and cell allocation in bovine embryos after in vitro production in contrasting culture systems. Reproduction 2002; 124: 155–165.
Orsi NM, Leese HJ. Amino acid metabolism of preimplantation bovine embryos cultured with bovine serum albumin or polyvinyl alcohol. Theriogenology 2004; 61: 561–572.