Cell-based therapy and rehabilitation with prosthetic limbs in a dog

A 5-year-old male dog was presented with the complaint of a chronic nonhealing wound on both hind legs. The wound occurred during a serious train accident that caused loss of both hind limbs below the hock joint. Since then, the wound did not respond to any standard treatment for up to 4 months. Again it was dressed for 15 days, but it did not show any improvement. With the consent of the owner, cell-based therapy was conducted by collecting bone marrow from the proximal anteromedial aspect of the tibia. After the application of bone marrow derived mesenchymal stem cells (BM-MSCs) the time to complete healing was 24 days. Due to pressure at the bony cut stump, the dog was noted to have pain while walking, and walked on his forelegs. An artificial limb was subsequently applied. The dog managed to walk normally after several days of training.

Cell-based therapy and rehabilitation with prosthetic limbs in a dog

A 5-year-old male dog was presented with the complaint of a chronic nonhealing wound on both hind legs. The wound occurred during a serious train accident that caused loss of both hind limbs below the hock joint. Since then, the wound did not respond to any standard treatment for up to 4 months. Again it was dressed for 15 days, but it did not show any improvement. With the consent of the owner, cell-based therapy was conducted by collecting bone marrow from the proximal anteromedial aspect of the tibia. After the application of bone marrow derived mesenchymal stem cells (BM-MSCs) the time to complete healing was 24 days. Due to pressure at the bony cut stump, the dog was noted to have pain while walking, and walked on his forelegs. An artificial limb was subsequently applied. The dog managed to walk normally after several days of training.

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  • Shrivastava PP, Chandrapuria VP, Bhargava MK, Malik MR. Efficacy of indigenous plants on healing of experimental wounds in cow calves: clinico-histomorphological and histochemical observations. Indian J Vet Surg 2002; 23: 45–46.
  • Bose VSC, Pattnaik TK, Bisoi PC, Mohapatra HK. Evaluation of Skin Heal spray for wound healing in domestic animals. Livestock International 2006; 11–16. 3. Harding KG, Morris HL, Patel GK. Healing of chronic wounds. Brit Med J 2002; 324: 160–163.
  • Falanga V. Wound healing and its impairment in the diabetic foot. Lancet 2005; 366: 1736–1743.
  • Singer AJ, Clark RAF. Cutaneous wound healing. New Engl J Med 1999; 341: 738–746.
  • Wu Y, Chen L, Scott G, Tredget EE. Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis. Stem Cells 2007; 25: 2648–2659.
  • Badiavas EV, Falanga V. Treatment of the chronic wounds with bone marrow-derived cells. Arch Dermatol 2003; 139: 510–516.
  • Bartsch T, Brehm M, Falke T, Kögler G, Wernet P, Strauer BE. Rapid healing of a therapy-refractory diabetic foot after transplantation of autologous bone marrow stem cells. Med Klin 2005; 100: 676–680 (article in German with an English abstract).
  • Dash NR, Dash SN, Routray P, Mohapatra S, Mohapatra PC. Targeting nonhealing ulcers of lower extremity in human through autologous bone marrow-derived mesenchymal stem cells. Rejuv Res 2009; 12: 359–365.
  • Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284: 143–147.
  • Crow SE, Walshaw SO. Manual of Clinical Procedures in the Dog, Cat, and Rabbit. 2nd ed. Hoboken, NJ, USA: Wiley; 1997.
  • Kasten P, Beyen I, Egermann M, Suda AJ, Moghaddam AA, Zimmermann G, Lubinbühl R. Instant stem cell therapy: characterization and concentration of human mesenchymal stem cells in vitro. Eur Cells Mater 2008; 16: 47–55.
  • Fossum TW, Hedlund CS, Hulse DA, Johnson AL, Seim HB, Willard MD, Carroll GL. Surgery of integumentary system. In: Welch Fossum T, Hedlund CS, editors. Manual of Small Animal Surgery. 2nd ed. Maryland Heights, MO, USA: Mosby; 1997. pp. 132–228.
  • Ghani A, Pandey SK, Kolte GN. Histological and histochemical evaluation of certain local medicaments as accelerator of wound healing. Indian J Indig Med. Suppl 1981; 2: 37–43.
  • Pascoe JR. Wound healing. In: Gourley IM, Vasseur PB, editors. General Small Animal Surgery. Philadelphia, PA, USA: Lippincott; 1985. pp. 105–119.
  • Curtis W. Wound healing. In: Slatter D, editor. Textbook of Small Animal Surgery. 2nd ed. Philadelphia, PA, USA: Saunders; 1993. pp. 53.
  • Singh H, Singh K. Wound healing and tissue repair. In: Tyagi RPS, Singh J, editors. Ruminant Surgery. 1st ed. New Delhi, India: CBS Publishers; 2010. pp. 58–71.
  • Borena BM, Pawde AM, Amarpal, Aithal HP, Kinjavdekar P, Singh R, Kumar D. Evaluation of healing potential of autologous bone marrow derived nucleated cells on incisional wounds in dogs. Indian J Vet Surg 2009; 30: 85–89.
  • Wu CH, Chang GY, Chang WC, Hsu CT, Che RS. Wound healing effects of porcine placental extracts on rats with thermal injury. Brit J Dermatol 2003; 148: 236–45.
  • Al-Khalidi A, Al-Sabiti H, Galipeau J, Lachapella K. Therapeutic angiogenesis using autologous bone marrow stromal cells improved blood flow in chronic limb ischemia model. Ann Thorac Surg 2003; 75: 204–209.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: 6
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Incidence and occurrence time of clinical mastitis in Holstein cows

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Alev Gürol BAYRAKTAROĞLU, Emel ERGÜN, Aytül KÜRÜM, Şevket ARIKAN, Özkan ŞİMŞEK

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Effect of dietary seaweed (Ulva lactuca) supplementation on growth performance of sheep and on in vitro gas production kinetics

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Effects of dystocia on milk production and reproduction in subsequent lactation in a Turkish Holstein herd

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Reference values for selected hematological and biochemical blood parameters from prepregnancy to advanced gestation in Angora cats

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Implementing innovative farm management practices on dairy farms: a review of feeding systems

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Histological and histochemical studies on the structure of pancreatic ductsof the goose (Anser anser)

Turgay DEPREM, Ebru SARI KARADAĞ, Seyit Ali BİNGÖL, Serap TAŞÇI KORAL, Şahin ASLAN, Serap İLHAN

Effects of alpha lipoic acid and vitamin C supplementation on transforming growth factor alpha (TGF a) and transforming growth factor beta 2 (TGF b2)secretion in testicular tissue of diabetic rats

Sevda ELİŞ YILDIZ, Buket BAKIR, Hamit USLU, Ebru KARADAĞ SARI, Musa KARAMAN