Biotyping and Serotyping of Mannheimia (Pasteurella) haemolytica Isolated from Lung Samples of Slaughtered Sheep in the Van Region
A total of 584 lung samples of slaughtered sheep having clinical symptoms of pneumonia were investigated microbiologically. Out of the 584 samples, 66 (11.3%) Mannheimia (Pasteurella) haemolytica strains were isolated on the basis of cultural, morphological and biochemical characteristics. Sixty-six isolates of M. haemolytica were biotyped by carbohydrate fermentation tests and serotyped by coagglutination and indirect haemagglutination (IHA) tests. Out of the 66 M. haemolytica strains, 57 (86.3%) were detected as biotype A, and 9 (13.6%) were biotype T. The 66 M. haemolytica isolates were serotyped as follows by coagglutination test: 13 (19.6%) A2, 11 (16.6%) A1, 9 (13.6%) A6, 7 (10.6%) A9, 4 (6.0%) A5, 4 (6.0%) A7, 4 (6.0%) A12, 3 (4.5%) A8, 1 (1.5%) T4, 1 (1.5%) A13, and 1 (1.5%) T15. Of the M. haemolytica strains, 14 (21.2%) belonged to serotype A2, 11 (16.6%) to serotype A1, 10 (15.1%) to serotype A6, 7 (10.6%) to serotype A9, 4 (6.0%) to serotype A5, 4 (6.0%) to serotype A7, 4 (6.0%) to serotype A12, 4 (6.0%) to serotype A8, 1 (1.5%) to serotype T4, 1 (1.5%) to serotype A13 and 1 (1.5%) to serotype T15 by IHA test. Eight (12.1%) and 5 (7.5%) isolates were unserotyped by coagglutination and IHA tests, respectively. T3, T10, A11, A14 and A16 serotypes were not determined.
Biotyping and Serotyping of Mannheimia (Pasteurella) haemolytica Isolated from Lung Samples of Slaughtered Sheep in the Van Region
A total of 584 lung samples of slaughtered sheep having clinical symptoms of pneumonia were investigated microbiologically. Out of the 584 samples, 66 (11.3%) Mannheimia (Pasteurella) haemolytica strains were isolated on the basis of cultural, morphological and biochemical characteristics. Sixty-six isolates of M. haemolytica were biotyped by carbohydrate fermentation tests and serotyped by coagglutination and indirect haemagglutination (IHA) tests. Out of the 66 M. haemolytica strains, 57 (86.3%) were detected as biotype A, and 9 (13.6%) were biotype T. The 66 M. haemolytica isolates were serotyped as follows by coagglutination test: 13 (19.6%) A2, 11 (16.6%) A1, 9 (13.6%) A6, 7 (10.6%) A9, 4 (6.0%) A5, 4 (6.0%) A7, 4 (6.0%) A12, 3 (4.5%) A8, 1 (1.5%) T4, 1 (1.5%) A13, and 1 (1.5%) T15. Of the M. haemolytica strains, 14 (21.2%) belonged to serotype A2, 11 (16.6%) to serotype A1, 10 (15.1%) to serotype A6, 7 (10.6%) to serotype A9, 4 (6.0%) to serotype A5, 4 (6.0%) to serotype A7, 4 (6.0%) to serotype A12, 4 (6.0%) to serotype A8, 1 (1.5%) to serotype T4, 1 (1.5%) to serotype A13 and 1 (1.5%) to serotype T15 by IHA test. Eight (12.1%) and 5 (7.5%) isolates were unserotyped by coagglutination and IHA tests, respectively. T3, T10, A11, A14 and A16 serotypes were not determined.
