Immunohistochemical Diagnosis of Aspergillosis in Adult Turkeys

The aim of the present study was to apply immunohistochemistry, using a commercially available monoclonal antibody, for the diagnosis of aspergillosis in adult turkeys. Eight adult turkeys were studied. The lungs revealed grossly a few yellowish-white granulomas. Histopathologically, the granulomas were distributed in the pulmonary parenchyma or occasionally located around the airways. A few fungal hyphae were detected within the necrotic debris of the granulomas in the lungs using routine fungal stains. Immunohistochemical staining performed on lung sections from all of the turkeys disclosed fungal hyphae and their fragments clearly within the necrotic debris and within the cytoplasm of macrophages and multinucleated foreign-body giant cells in the granulomas. In conclusion, even though fungal elements within the granulomas with histologic characteristics are indicative of the disease, immunohistochemistry should be applied to ensure a reliable diagnosis of aspergillosis in adult turkeys, and to differentiate it from other filamentous mycelia.

Anahtar Kelimeler:

Turkey, aspergillosis, immunohistochemistry

Immunohistochemical Diagnosis of Aspergillosis in Adult Turkeys

Keywords:

Turkey, aspergillosis, immunohistochemistry,

PDF

___

Piérard, G.E., Arrese Estrada, J., Piérard-Franchimont, C., Thiry, A., Stynen, D.: Immunohistochemical expression of galactomannan in the cytoplasm of phagocytic cells during invasive aspergillosis. Am. J. Clin. Pathol., 1991; 96: 373-376. 2.
Buckley, H.R., Richardson, M.D., Evans, E.G., Wheat, L.J.:
Immunodiagnosis of invasive fungal infection. J. Med. Vet. Mycol.,
Jensen, H.E., Aalbæk, B., Lind, P., Frandsen, P.L., Krogh, H.V., Stynen, D.: Enzyme immunohistochemistry with mono- and polyclonal antibodies in the pathological diagnosis of systemic bovine mycoses. Acta Pathol. Microbiol. Immunol. Scand., 1993; 101: 505-516.
Jensen, H.E., Christensen, J.P., Bisgaard, M., Nielsen, O.L.: Immunohistochemistry for the diagnosis of aspergillosis in turkey poults. Avian Pathol., 1997; 26: 5-18.
Hsu, S.M., Raine, L., Fanger, H.: Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem., 1981; 29: 577-580.
Richard, J.L.: Aspergillosis. In: Calnek, B.W., Barnes, H., Beard, C.W., Reid, W.M., Yoder, H.W., Eds. Diseases of Poultry. Wolfe Publishing Ltd, London, 1991; 326-334.
Singh, N., Grewal, G.S., Brar, R.S., Singh, B., Dhaliwal, A.S., Jand, S.K.: Outbreak of mycotic tracheitis in domestic fowl. Mycoses, 1993; 36: 65-67.
Machin, K., Langelier, K.M., Lewis, R.J.: Aspergillosis outbreak in Stellar’s jays (Cyanocitta stelleri) from central Vancouver Island. Can. Vet. J., 1993; 34: 247-248.
Hawkey, C.M., Pugsley, S.L., Knight, J.A.: Abnormal heterophils in a king shag with aspergillosis. Vet. Rec., 1984; 114: 322-324.
Reece, R.L., Taylor, T.K., Dickson, D.B., Kerr, P.J.: Mycosis of commercial Japanese quail, ducks and turkeys. Aust. Vet. J., 1986; 63: 196-197.
Beytut, E., Ozcan, K., Erginsoy, S.: Immunohistochemical detection of fungal elements in the tissues of goslings with pulmonary and systemic aspergillosis. Acta. Vet. Hung., 2004; 52: 71-84.
Pal, M., Gangopadhyay, R.M., Prajapati, K.S.: Systemic aspergillosis in chicks due to Aspergillus flavus. Indian J. Anim. Sci., 1989; 59: 1074-1075.
Pandita, A., Sadana, J.R., Asrani, R.K.: Studies on clinical signs and haematological alterations in pneumonic aspergillosis due to Aspergillus flavus in Japanese quail. Mycopathologia, 1991; 116: 119-123.
Chute, H.L.: Diseases caused by fungi. In: Biester, H.E., Schwarte, L.H., Eds. Diseases of Poultry. The Iowa State University Press, Iowa, 1965; 494-502.
Okoye, J.O.A., Okeke, C.N.: Pathogenicity of an isolate of Aspergillus flavus in chickens. Avian Pathol., 1986; 15: 259-270.
Sancak, A.A., Paracıkoğlu, J.: Aspergillosis and gastric impaction in an ostrich. Turk. J. Vet. Anim. Sci., 2005; 29: 933-935.
Jensen, H.E., Schonheyder, H.: Immunofluorescence staining of hyphae in the histopathological diagnosis of mycoses in cattle. J. Med. Vet. Mycol., 1989; 27: 33-44.