Morphological Studies on Ovarian Mast Cells in the Cow
This study was conducted on cows in the estrual and luteal phases of the cycle to determine the staining properties, light and electron microscopic appearance and numerical distribution of mast cells from various areas of the ovary. The material for the study comprised ovarian specimen taken from 14 cows, 7 in the luteal phase and 7 in the estrual phase of the sexual cycle. Specimens were taken from 3 different areas of the ovaries, including those surrounding the corpus luteum, the graafian follicle and the medulla. From all 3 areas, mast cells were demonstrated as metachromatic staining after treatment of paraffin-embedded sections with toluidine blue and as Ab(+)/SO(-) by the combined alcian blue/safranine O (Ab/SO) stain. In the electron microscopic studies, the mast cells were observed to have 2 types of granules, namely homogeneously dense granules and tiny particulate granules. All 3 regions of the ovary were observed to have higher average mast cell counts per mm2 during the estrual phase than during the luteal phase (P < 0.001). In the estrual phase, while mast cell counts demonstrated a marked increase in the periphery of the graafian follicle, a low number of mast cells was determined in the periphery of the corpus luteum. In comparison, the medulla was demonstrated to have a much higher concentration of mast cells in the medulla than the other 2 regions of the ovarium.
Morphological Studies on Ovarian Mast Cells in the Cow
This study was conducted on cows in the estrual and luteal phases of the cycle to determine the staining properties, light and electron microscopic appearance and numerical distribution of mast cells from various areas of the ovary. The material for the study comprised ovarian specimen taken from 14 cows, 7 in the luteal phase and 7 in the estrual phase of the sexual cycle. Specimens were taken from 3 different areas of the ovaries, including those surrounding the corpus luteum, the graafian follicle and the medulla. From all 3 areas, mast cells were demonstrated as metachromatic staining after treatment of paraffin-embedded sections with toluidine blue and as Ab(+)/SO(-) by the combined alcian blue/safranine O (Ab/SO) stain. In the electron microscopic studies, the mast cells were observed to have 2 types of granules, namely homogeneously dense granules and tiny particulate granules. All 3 regions of the ovary were observed to have higher average mast cell counts per mm2 during the estrual phase than during the luteal phase (P < 0.001). In the estrual phase, while mast cell counts demonstrated a marked increase in the periphery of the graafian follicle, a low number of mast cells was determined in the periphery of the corpus luteum. In comparison, the medulla was demonstrated to have a much higher concentration of mast cells in the medulla than the other 2 regions of the ovarium.
___
- Dubois, J.A., Wordinger, R.J., Dickey, J.F.: Tissue concentrations of mast cells and lymphocytes of the bovine uterine tube (oviduct) during the estrous cycle. Am. J. Vet. Res., 1980; 41: 806-808.
- Özen, A., Afltı, R.N., Kurtdede, N.: Light and electron microscopic studies on mast cells on the bovine oviduct. Dtsch. Tierärztl. Wochenschr., 2002; 109: 412-415.
- Likar, I.N., Likar, L.J.: Acid mucopolysaccharides and mast cells in the bovine uterus at different stages of the sexual cycle. Acta Endocrinol. (Copenh.), 1964; 46: 493-506.
- Reimers, T.J.: Laboratory training manual on radioimmunoassay in animal reproduction. FAO/IAEA division, technical report series. 1984; 233: 85-111.
- Enerbäck, L.: Mast cells in rat gastrointestinal mucosa. 1. Effects of fixation. Acta Pathol. Microbiol. Scand., 1966; 66: 289-302.
- Enerbäck, L.: Mast cells in rat gastrointestinal mucosa. 2. Dye- binding and metachromatic properties. Acta Pathol. Microbiol. Scand., 1966; 66: 303-312.
- Culling, C.F.A., Allison, R.T., Barr, W.T.: Cellular Pathology Technique. Fourth edition, Butterworth, Wellington, 1985.
- Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol., 1965; 27: 137A-138A.
- Veneable, J.H. Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol., 1965; 25: 407-408.
- Böck, P.: Romeis Microskopische Tecknik. 17. Aufl. Urban und Scwarzenberg. München. 1989.
- Sümbüloğlu, K. Sümbüloğlu, V.: Biyoistatistik, Hatipoğlu Yayınları, Ankara. 2000.
- Chen, W., Alley, M.R., Manktelow, B.W., Davey, P.: Mast cells in the ovine lower respiratory tract: heterogeneity, morphology and density. Int. Arch. Allergy Appl. Immunol., 1990; 93: 99-106.
- Chen, W., Alley, M.R., Manktelow, B.W., Slack, P.: Mast cells in the bovine lower respiratory tract: morphology, density and distribution. Br. Vet. J., 1990; 146: 425-436.
- Eren, Ü., Afltı, R.N., Kurtdede, N., Sandıkçı, M., Sur, E.: İnek uterusunda mast hücrelerinin histolojik ve histokimyasal özellikleri ve mast hücre heterojenitesi. Turk. J. Vet. Anim. Sci., 1999; 23: 193-201.