Investigation of Some Biochemical Parameters and the Antioxidant System in Calves with Dermatophytosis

The study included 10 clinically healthy calves (group 1) and 10 clinical cases of dermatophytosis (group 2). The influence of dermatophytosis on some serum biochemical profiles and the antioxidant system was investigated. Serum malondialdehyde (MDA), reduced glutathione (GSH), uric acid, nitric oxide (NO), total sialic acid (TSA), lipid-bound sialic acid (LBSA), total protein (TP), and albumin levels were measured in groups 1 and 2. Calves in group 2 had significantly higher (P < 0.001) MDA, NO, TSA, and LBSA levels, and a higher P < 0.01 TSA:TP ratio; however, GSH and uric acid levels were significantly lower (P < 0.001). There was no significant difference between the groups in total protein and albumin. These findings suggest a relationship between dermatophytosis, the antioxidant system, and lipid oxidation.

Investigation of Some Biochemical Parameters and the Antioxidant System in Calves with Dermatophytosis

The study included 10 clinically healthy calves (group 1) and 10 clinical cases of dermatophytosis (group 2). The influence of dermatophytosis on some serum biochemical profiles and the antioxidant system was investigated. Serum malondialdehyde (MDA), reduced glutathione (GSH), uric acid, nitric oxide (NO), total sialic acid (TSA), lipid-bound sialic acid (LBSA), total protein (TP), and albumin levels were measured in groups 1 and 2. Calves in group 2 had significantly higher (P < 0.001) MDA, NO, TSA, and LBSA levels, and a higher P < 0.01 TSA:TP ratio; however, GSH and uric acid levels were significantly lower (P < 0.001). There was no significant difference between the groups in total protein and albumin. These findings suggest a relationship between dermatophytosis, the antioxidant system, and lipid oxidation.

___

  • Erdoğan, H.M., Çitil, M., Günefl, V., Saatci, M.: Dairy cattle farming in Kars district, Turkey: I. characteristics and production. Turk. J. Vet. Anim. Sci., 2004; 28: 735-743.
  • Halley, L.D., Standard, P.G.: Laboratory Methods in Medical Mycology, 3rdEd. pp 41-57. US Department of Health, Education and Welfare, Center of Disease Control, Atlanta, USA, 1973.
  • Sydow, G.: A simplified quick method for determination of sialic acid in serum. Biomed. Biochim. Acta, 1985; 44: 1721-1723.
  • Katopodis, N., Stock, C.C.: Improved method to determine lipid bound sialic acid in plasma or serum. Res. Commun. Chem. Pathol. Pharmacol., 1980; 30: 171-180.
  • Yoshioka, T., Kawada, K., Shimada, T., Mori, M.: Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am. J. Obstet. Gynecol., 1979; 135: 372-376.
  • Cortas, N.K., Wakid, N.W.: Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin. Chem., 1990; 36: 1440-1443.
  • Beutler, E., Duron, O., Kelly, B.M.: Improved method for the determination of blood glutathione. J. Lab. Clin. Med., 1963; 61: 882-888.
  • Radostits, O.M., Blood, D.C., Gay, C.C.: Veterinary Medicine, 8th Ed. Bailliere Tindall, London, UK, 1994, 381-390.
  • Metodiewa, D., Koska, C.: Reactive oxygen species and reactive nitrogen species: relevance to cyto(neuro)toxic events and neurologic disorders. An overview. Neurotoxicol. Res., 2000; 1: 197-233.
  • Roth, E.: Oxygen free radicals and their clinical implications. Acta Chir. Hung., 1997; 36: 302-305.
  • Castillo, C., Hernandez, J., Bravo, A., Lopez-Alonso, M., Pereira, V., Benedito, J.L.: Oxidative status during late pregnancy and early lactation in dairy cows. Vet. J., 2005; 169: 286-292.
  • Thomas, C.E., Kalyanaraman, B.: Oxygen Radicals and the Disease Process. Harwood Academic Publishers, Amsterdam, the Netherlands, 1997.
  • Erel, O., Kocyigit, A., Avci, S., Aktepe, N., Bulut, V.: Oxidative stress and antioxidative status of plasma and erythrocytes in patients with vivax malaria. Clin. Biochem., 1997; 30: 631-639.
  • Vazquez-Torres, A., Balish, E.: Macrophages in resistance to candidiasis. Microbiol. Mol. Biol. Rev., 1997; 61: 170-192.
  • Koca, R., Armutcu, F., Altinyazar, H.C., Gurel, A.: Oxidant- antioxidant enzymes and lipid peroxidation in generalized vitiligo. Clin. Exp. Dermatol., 2004; 29: 406-409.
  • Ghiselli, A., Serafini, M., Natella, F., Scaccini, C.: Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radic. Biol. Med., 2000; 29: 1106-1114.
  • Castillo, C., Hernandez, J., Lopez-Alonso, M., Miranda, M., Benedito, J.L.: Values of plasma lipid hydroperoxides and total antioxidant status in healthy dairy cows: preliminary observations. Arch. Tierz., 2003; 46: 227-233.
  • Wayner, D.D., Burton, G.W., Ingold, K.U., Barclay, L.R., Locke, S.J.: The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma. Biochim. Biophys. Acta, 1987; 924: 408-419.
  • Kolo, L.L., Westfall, T.C., Macarthur, H.: Nitric oxide decreases the biological activity of norepinephrine resulting in altered vascular tone in the rat mesenteric arterial bed. Am. J. Physiol. Heart Circ. Physiol., 2004; 286: 296-303.
  • Keilhoff, G.: Foreword basic research on nitric oxide (NO). Cell. Mol. Biol. (Noisy-le-grand), 2005; 51: 245.
  • Carrillo-Vico, A., Lardone, P.J., Naji, L., Fernandez-Santos, J.M., Martin-Lacave, I., Guerrero, J.M., Calvo, J.R.: Beneficial pleiotropic actions of melatonin in an experimental model of septic shock in mice: regulation of pro-/anti-inflammatory cytokine network, protection against oxidative damage and anti-apoptotic effects. J. Pineal Res., 2005; 39: 400-408.
  • Dogan, H., Pasaoglu, H., Ekinciler, O.F., Tatlisen, N.: A comparative study of total protein, total and lipid associated serum sialic acid levels in patients with Behcet's disease and control groups. Acta Ophthalmol. (Copenhagen), 1992; 70: 790–794.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Haemato-Biochemical Aspects of Parturient Haemoglobinuria in Buffalo

Muhammad Zubair AKHTAR, Ahrar KHAN, Muhammad Zargham KHAN, Ghulam MUHAMMAD

Investigation of Some Biochemical Parameters and the Antioxidant System in Calves with Dermatophytosis

Mahmut KARAPEHLİVAN, Erdoğan UZLU, Necati KAYA, Orhan KANKAVİ

Presence of a Very Virulent Genotype of Infectious Bursal Disease Virus in Vaccinated Layer Hens in Turkey

Ali Osman ÇERİBAŞI, Hakan BULUT, İrem GÜLAÇTI, Yesari ERÖKSÜZ, Yusuf BOLAT

Immunohistochemical Diagnosis of Aspergillosis in Adult Turkeys

Enver BEYTUT

Morphological Studies on Ovarian Mast Cells in the Cow

Asuman ÖZEN, Levent ERGÜN, Emel ERGÜN, Nejdet ŞİMŞEK

Evaluation of the Treatment of Repeat Breeder Dairy Cows with Uterine Lavage plus PGF2a , with and without Cephapirin

Mohammad Rahim AHMADI, Seyed Asghar DEHGHAN

Protein and Lipid Content and Fatty Acid Composition of Anchovy Meal Produced in Turkey

Hülya TURAN, Yalçın KAYA, İbrahim ERKOYUNCU

Biotyping and Serotyping of Mannheimia (Pasteurella) haemolytica Isolated from Lung Samples of Slaughtered Sheep in the Van Region

Ziya İLHAN, İhsan KELEŞ

Unusual Case of Senecio sceleratus (Ragwort) Poisoning in an Ostrich (Struthio camelus) in Zimbabwe

Ross Gordon COOPER

Ulva rigida ve Cystoseira barbata Unlarının Yem Katkı Maddesi Olarak Nil tilapya Balıklarında Büyüme Performansı, Yem Kullanımı ve Vücut Kompozisyonu Üzerine Etkileri

Derya GÜROY, Feyza SANVER, Kut Betül GÜROY, Şükran CİRİK, Ahmet Adem TEKİNAY