A phylogenetic analysis of Mycoplasma strains circulating in sheep pneumonia in the Kars region of Turkey
A phylogenetic analysis of Mycoplasma strains circulating in sheep pneumonia in the Kars region of Turkey
This study aimed to investigate Mycoplasma species by cultural and molecular methods in 250 pneumonics and 30 healthy appearance sheep lungs brought from slaughterhouse and butcheries in the Kars region of Turkey. The phylogenetic positioning of the bacterial strains to be obtained in the present study was another remarkable outcome of the study. Mycoplasma isolation was achieved solely in 26 (10.4%) of 250 sheep lungs with pneumonia. Mycoplasma ovipneumoniae was found predominant species in 12 (46.15%) of samples and that was followed by Mycoplasma arginini in 4 (15.38%) of strains. Moreover, two samples were found to be co-infected with the aforementioned Mycoplasma species and 8 were found negative. The partial sequence analysis of the 16S rRNA gene of 22 (84.61%) isolates emerged that 11 of the isolates were M. ovipneumoniae and 4 were M. arginini. Two mixed samples were further identified as M. arginini by the sequence analysis. Five isolates that were not identified by cultural and PCR procedures were identified as M. bovigenitalium by sequence analysis. The evolutionary analysis of the 16S rRNA sequence yielded three main clusters as M. ovipneumoniae, M. arginini, and M. bovigenitalium which took part in the Hominis group that was previously described in Mycoplasma phylum. The inter se proximity of the main clusters was thus described polyphyletic for M. ovipneumoniae and M. arginini and monophyletic for M. bovigenitalium. However, in the general view, M. arginini and M. bovigenitalium isolates had two distinct phylogenetic positions, but their proximity to each other was higher than those obtained for M. ovipneumoniae isolates.
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- 1. USDA. Sheep and Lamb Nonpredator Death Loss in the United States, 2009. Fort Collins, CO. 591.0511, 2010.
- 2. Tabatabayi AH, Gharagozlou MJ, Ghader- Sohi A. A survey of Mycoplasma arginini and other agents from subacute and chronic ovine pneumonia in Iran. Preventive Veterinary Medicine 1992; 12: 153-158.
- 3. Otlu S. Kars yöresinde koyun pnömonilerinden mikoplazmaların izolasyonu, identifikasyonu ve antibiyotiklere olan duyarlılıklarının belirlenmesi. PhD Dissertation, Kafkas University, Kars, Turkey, 1996 (in Turkish).
- 4. Lefevre PC, Jones GE, Ojo MO. Pulmonary mycoplasmosis of small ruminants. Revue Scientifique Et Technique (International Office of Epizootics) 2004; 6: 759-799.
- 5. Ayling RD, Bashiruddin SE, Nicholas RAJ. Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Veterinary Record 2004; 155, 413-416.
- 6. Marmur J. A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 1961; 3: 208-218.
- 7. Botes A, Peyrot BM, Olivier AJ, Burger WP, Bellstedt DU. Identification of three novel mycoplasma species from ostriches in South Africa. Veterinary Microbiology 2005; 111: 159-169.
- 8. Kılıc A, Kalender H, Eroksuz H, Muz A, Tasdemir B. Identification by culture, PCR, and immunohistochemistry of mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey. Tropical Animal Health and Production 2013; 45: 1525-1531.
- 9. Clinical and Laboratory Standards Institute (CLSI). Interpretive Criteria for Identification of Bacteria and Fungi by DNA Target Sequencing; Approved Guideline. In: Institute CaLS (Ed). CLSI Document MM18- A. Wayne, PA, USA: CLSI; 2008.
- 10. Dubey JP, Lindsay DS. Neospora caninum induced abortion in sheep. The Journal of Veterinary Diagnostic Investigation 1990; 2: 230-233.
- 11. Büyük F, Çelebi Ö, Şahin M, Ünver A, Tazegül E. Brucella and Campylobacter mixed infection in two different sheep and goat herds. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2011; 17: 177-180.
- 12. Arslan S, Öncel T, Malal ME, Satır E, Sait A et al. Bacteriological, virological and parasitological etiology in diarrhea cases in determined in post-mortem lambs and kids in Marmara Region. Van Veterinary Journal 2016; 27: 147-152.
- 13. Ikede BO. The pattern of respiratory lesion in goats and sheep in Nigeria, Part II--lesions in sheep. The Bulletin of Animal Health and Production in Africa 1978; 26: 172-185.
- 14. Martin WB. Respiratory infections of sheep. Comparative Immunology, Microbiology & Infectious Diseases 1996; 19: 171-179.
- 15. Di Provvido A, Averaimo D, Zilli K, Marruchella G, Scacchia M. Mycoplasma pneumonia in small ruminants: a ten- year long retrospective survey. Small Ruminant Research 2017; 153: 103-106.
- 16. Al- Aubaidi JM, Taylor WD, Bubash GR, Dardiri AH. Identification and characterization of Mycoplasma arginini from Bighorn sheep (Ovis canadensis) and goats. American Journal of Veterinary Research 1972; 33: 87-90.
- 17. Thurley DC, Boyes BW, Davies DH, Wilkins MF, O’ Connell E et al. Subclinical pneumonia in lambs. The New Zealand Veterinary Journal 1977; 25: 173-176.
- 18. Bakke T. The occurrence of Mycoplasmas and bacteria in lungs from sheep in Southern Norway. Acta Veterinaria Scandinavica 1982; 23: 235-247.
- 19. St George TD. Investigations of respiratory disease of sheep in Australia. Australian Veterinary Journal 1972; 48: 318-322.
- 20. Pasic S, Popovic M. The study of ovine Mycoplasmas in Bosnia and Herzegovina (Yugoslavia): 2. enriched media for isolation of ovine Mycoplasmas. Veterinaria 1989; 38: 307-311.
- 21. Aksoy E. Etlik Hayvan Hastalıkları Araştırma Enstitüsü’ne gelen koyun materyallerinde Mikoplazmal enzootik pnöymoni olayları ve patolojik bulgular. Etlik Veteriner Mikrobiyoloji Dergisi 1993; 7: 172-197 (in Turkish).
- 22. Erken N. The isolation and identification of Mycoplasma species in sheep and lambs that indicates pneumonia in Samsun region. MSc Thesis, Samsun Veterinary Control and Research Institute, Samsun, Turkey, 2004.
- 23. Sheehan M, Casidy JP, Brady J, Ball H, Doherty ML et al. An aetiopathological study of chronic bronchopneumonia in lambs in Ireland. The Veterinary Journal 2007; 173: 630-637.
- 24. Lin YC, Miles RJ, Nicholas RAJ, Kelly DP, Wood AP. Isolation and immunological detection of Mycoplasma ovipneumoniae in sheep with atypical pneumonia, and lack of a role for Mycoplasma arginini. Research in Veterinary Science 2008; 84: 367-373.
- 25. Nicholas R, Ayling R, McAuliffe L. Mycoplasma Diseases of Ruminants: Disease, Diagnosis and Control. Cambridge, MA, USA: CABI; 2008.
- 26. Güler L. Pnömonili koyun ve keçilerden mikoplazmaların izolasyonu, identifikasyonu ve antibiyotiklere olan duyarlılıklarının belirlenmesi.PhD Dissertation, Selçuk University, Konya, Turkey, 1993 (in Turkish).
- 27. Abdel Halium MM, Salib FA, Marouf SA, Abdel Massieh ES. Isolation and molecular characterization of Mycoplasma spp. in sheep and goats in Egypt. Veterinary World 2019; 12: 664- 670.
- 28. Adehan RK, Ajuwape ATP, Adetosoye AI, Alaka OO. Characterization of Mycoplasmas isolated from pneumonic lungs of sheep and goats. Small Ruminant Research 2006; 63: 44-49.
- 29. Ball HJ. Experimental mastitis caused by Mycoplasma bovigenitalium and M. canadense in the ewe. Veterinary Microbiology 1990; 22: 383-388.
- 30. Kalshingi HA, Bosman AM, Gouws J, Van Vuuren M. Molecular characterisation of Mycoplasma species isolated from the genital tract of Dorper sheep in South Africa. The Journal of the South African Veterinary Association 2015; 86: 1-11.
- 31. Turan MK, Günay ÖC, Kayış SA, Çörtük M. 16S rRNA in microbiota and basic bioinformatics analysis. Journal of Biotechnology and Strategic Health Research 2018; 2: 23-34.
- 32. Azizi S, Tajbakhsh E, Rezaii A, Nekouei SH, Namjoo AR. The role of Mycoplasma ovipneumoniae and Mycoplasma arginini in pneumonic lungs of slaughtered sheep. Revue de Médecine Vétérinaire 2011; 162: 310-315.
- 33. Weisburg WG, Tully JG, Rose DL, Petzel JP, Oyaizu H et al. A phylogenetic analysis of the Mycoplasmas: basis for their classification. Journal of Bacteriology 1989; 171: 6455-6467.
- 34. Maniloff J. Phylogeny and Evolution. In: Razin S, Herrmann R (editors). Molecular Biology and Pathogenicity of Mycoplasmas. New York, NY, USA: Kluwer Academic/Plenum Publishers, 2002.