Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid

Background/aim: To evaluate acetylcholinesterase (AChE) inhibitory activity and antioxidant capacity of the major molecule from Salvia sp., rosmarinic acid, as a drug candidate molecule for treatment of Alzheimer disease (AD). Materials and methods: The AChE inhibitory activity of different extracts from Salvia trichoclada, Salvia verticillata, and Salvia fruticosa was determined by the Ellman and isolated guinea pig ileum methods, and the antioxidant capacity was determined with DPPH. The AChE inhibitory activity of the major molecule rosmarinic acid was determined by in silico docking and isolated guinea pig ileum methods. Results: The methanol extract of Salvia trichoclada showed the highest inhibition on AChE. The same extract and rosmarinic acid showed significant contraction responses on isolated guinea pig ileum. All the extracts and rosmarinic acid showed high radical scavenging capacities. Docking results of rosmarinic acid showed high affinity to the selected target, AChE. Conclusion: In this study in vitro and ex vivo studies and in silico docking research of rosmarinic acid were used simultaneously for the first time. Rosmarinic acid showed promising results in all the methods tested.

Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid

Background/aim: To evaluate acetylcholinesterase (AChE) inhibitory activity and antioxidant capacity of the major molecule from Salvia sp., rosmarinic acid, as a drug candidate molecule for treatment of Alzheimer disease (AD). Materials and methods: The AChE inhibitory activity of different extracts from Salvia trichoclada, Salvia verticillata, and Salvia fruticosa was determined by the Ellman and isolated guinea pig ileum methods, and the antioxidant capacity was determined with DPPH. The AChE inhibitory activity of the major molecule rosmarinic acid was determined by in silico docking and isolated guinea pig ileum methods. Results: The methanol extract of Salvia trichoclada showed the highest inhibition on AChE. The same extract and rosmarinic acid showed significant contraction responses on isolated guinea pig ileum. All the extracts and rosmarinic acid showed high radical scavenging capacities. Docking results of rosmarinic acid showed high affinity to the selected target, AChE. Conclusion: In this study in vitro and ex vivo studies and in silico docking research of rosmarinic acid were used simultaneously for the first time. Rosmarinic acid showed promising results in all the methods tested.

Kaynakça

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Kaynak Göster

Bibtex @ { tbtkmedical149057, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2015}, volume = {45}, pages = {1141 - 1148}, doi = {}, title = {Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid}, key = {cite}, author = {Demirezer, Lütfiye Ömür and Gürbüz, Perihan and Uğur, Emine Pelin Kelicen and Bodur, Mine and Özenver, Nadire and Uz, Ayşe and Güvenalp, Zühal} }
APA Demirezer, L , Gürbüz, P , Uğur, E , Bodur, M , Özenver, N , Uz, A , Güvenalp, Z . (2015). Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid . Turkish Journal of Medical Sciences , 45 (5) , 1141-1148 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/12392/149057
MLA Demirezer, L , Gürbüz, P , Uğur, E , Bodur, M , Özenver, N , Uz, A , Güvenalp, Z . "Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid" . Turkish Journal of Medical Sciences 45 (2015 ): 1141-1148 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/12392/149057>
Chicago Demirezer, L , Gürbüz, P , Uğur, E , Bodur, M , Özenver, N , Uz, A , Güvenalp, Z . "Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid". Turkish Journal of Medical Sciences 45 (2015 ): 1141-1148
RIS TY - JOUR T1 - Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid AU - Lütfiye Ömür Demirezer , Perihan Gürbüz , Emine Pelin Kelicen Uğur , Mine Bodur , Nadire Özenver , Ayşe Uz , Zühal Güvenalp Y1 - 2015 PY - 2015 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 1141 EP - 1148 VL - 45 IS - 5 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Medical Sciences Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid %A Lütfiye Ömür Demirezer , Perihan Gürbüz , Emine Pelin Kelicen Uğur , Mine Bodur , Nadire Özenver , Ayşe Uz , Zühal Güvenalp %T Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid %D 2015 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 45 %N 5 %R %U
ISNAD Demirezer, Lütfiye Ömür , Gürbüz, Perihan , Uğur, Emine Pelin Kelicen , Bodur, Mine , Özenver, Nadire , Uz, Ayşe , Güvenalp, Zühal . "Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid". Turkish Journal of Medical Sciences 45 / 5 (Aralık 2015): 1141-1148 .
AMA Demirezer L , Gürbüz P , Uğur E , Bodur M , Özenver N , Uz A , Güvenalp Z . Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid. Turkish Journal of Medical Sciences. 2015; 45(5): 1141-1148.
Vancouver Demirezer L , Gürbüz P , Uğur E , Bodur M , Özenver N , Uz A , Güvenalp Z . Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid. Turkish Journal of Medical Sciences. 2015; 45(5): 1141-1148.
IEEE L. Demirezer , P. Gürbüz , E. Uğur , M. Bodur , N. Özenver , A. Uz ve Z. Güvenalp , "Molecular docking and ex vivo and in vitro anticholinesterase activity studies ofSalvia sp. and highlighted rosmarinic acid", Turkish Journal of Medical Sciences, c. 45, sayı. 5, ss. 1141-1148, Ara. 2015

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