The efficacy, physiological responses and hematology of persian sturgeon, acipenser persicus, to clove oil as an anesthetic agent

İran kökenli mersin balığında Acipenser persicus anestezik olarak karanfil yağının kullanılabilirliğini değerlendirmek amacıyla deneme yapılmıştır. Aynı balık türünde karanfil yağının normal plazma kortizonunu ve glikoz seviyesini değiştirmesi ve karanfil yağının hematolojik faktörlere (Hb, Hct, and WBC) olan etkisi de araştırılmıştır. İki su sıcaklığına (20 ve 24°C) karşın üç farklı karanfil yağı dozu (200, 300, 400 mg/L) kullanılmıştır. 400 mg/L ve 24°C kombinasyonu hem anestezi hem de uyanma için en iyi muamele olarak değerlendirilmiştir. 300 mg/L ve 24°C kombinasyonundaki balıklardan ayılmalarından sonraki 1, 6, 24 ve 72. saatlerde, fizyolojik tepkilerini değerlendirmek amacıyla kan örnekleri alınmıştır. 72. saatin sonunda hem kan glikoz seviyesi hem de kortizon seviyeleri kontrol grubuyla aynı seviyelere ulaşmıştır. Hematolojik parametreler hafif dalgalanmaların ardından kontrol grubuyla aynı seviyelere ulaşmıştır.

Anahtar Kelimeler:

kan kimyası, hemoglobin, Acipenser, anestetikler, anestezi, hayvan fizyolojisi, kan şekeri, karanfil, hematokrit, hidrokortizon, akyuvar sayısı, bitkisel yağlar, mersin balığı

İran mersin balığının, acipenser persicus, anestezik ajan olarak karanfil yağına, efikasitesi, fizyolojik tepkileri ve hematolojisi

In order to examine the efficacy of clove oil as an anesthetic on Persian sturgeon, Acipenser persicus, we conducted an experiment. We also investigate if clove oil anesthesia suppressed the normal plasma cortisol and glucose level increase in this species or not. The Effects of clove oil on Hematological factors (Hb, Hct, and WBC) was examined as well. Three different concentrations of clove oil (200, 300, 400 mg/L) combined the two water temperature (20 and 24°C) were assessed. The combination of 400 mg/L and 24°C was the best treatment for anesthetizing as well as recovery. From one trial (concentration: 300 mg/L and temperature: 24°C), blood samples were taken on 0 (immediately upon placement in recovery tank), 1, 6, 24 and 72 hours post recovery in order to evaluate physiological responses. After 72 h both blood glucose and cortisol reach the level similar to the control group which was before applying clove oil. Hematological parameters after some fluctuation reached its level the same as control group of the experiments.

Keywords:

blood chemistry, haemoglobin, Acipenser, anaesthetics, anaesthesia, animal physiology, blood sugar, cloves, haematocrit, hydrocortisone, leukocyte count, plant oils, sturgeons,

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