Md. Shahidul HAQUE, Swapan Kumar ROY, M. SHAHJAHAN

Arsenic impairs the effect of low temperature on the regulation of $Na^+-K^+$ atpase activity in skeletal muscle of fish ( channa punctata)

Channa punctata'nm. metabolizmasının düşük çevre sıcaklığına ihtiyaç duyup duymadığını belirlemek için, balıklar 4~8°C'de 30 dakika, 1 ve 2 saat tutulmuştur. Bir saatin sonunda, normal şartlarda tutulan kontrole kıyasla, soğukta tutulan balıklarda iskelet kasındaki inorganik fosfat (Pi) birikiminin önemli derecede arttığı buna rağmen, iki saatin sonunda soğuğa karşı verilen tepkinin azaldığı gözlemlenmiştir. Ayrı ayrı yapılan denemelerde iskelet kası ekstrakt mdaki $Na^+-K^+$ ATPase aktivitelerinin 1. ve 2. saatin sonunda önemli derecede arttığı belirlenmiştir. Sonuçlar, soğuğa karşı artan Pi'nin $Na^+-K^+$ ATPase'm yüksek aktivitesine bağlı olduğunu göstermektedir. Balık grupları, enzimin düzenleyici mekanizmasını netleştirmek için 1 saat ve 2 saat boyunca soğuk ile birlikte 100 mM Na2HAs04'e maruz bırakılmıştır. Artan $Na^+-K^+$ ATPase aktivitesi $Na{_2HAsO_4}$ ile muamele edilince kontrole kıyasla önemli derecede azalmıştır. Artan $Na^+-K^+$ ATPaz aktivitesi, $Na{_2HAsO_4}$ ile muamele edildiğinde sırasıyla kontrol grubuna ve soğuğa maruz kalan balıklara kıyasla önemli derecede azalmıştır. Deneysel bulgular, soğuk indüklenmiş $Na^+-K^+$ ATPaz aktivitesinin düzeninde arsenikin gerekli olduğunu göstermiştir. Bu türün iskelet kasındaki soğukta artan $Na^+-K^+$ ATPaz aktivitesi ve bunun arsenik ile inhibisyonu çevre için kritik bir rol oynayabilir.

Anahtar Kelimeler:

toksisite, maruz kalma, inorganik fosfor, metabolizma, potasyum, iskelet kası, su sıcaklığı, toksikoloji, Channa punctatus, adenozin trifosfataz, arsenik, enzim aktivitesi, sodyum

Arseniğin channa punctata'nın iskelet kasında $Na^+-K^+$ atpaz aktivitesi düzenine düşük sıcaklığın etkilerini zayıflatması

To characterize whether environmental low temperature is involved in metabolic regulation, Channa punctata species of fish were exposed to cold (4~8°C) for 30 min, 1 hr and 2 hrs since they are much energetic and survive in the critical environment. The inorganic phosphate (Pi) in skeletal muscle was significantly increased after 1 hr in cold compared to control fish kept in ambient temperature, however, although lower, stimulatory response to cold for 2 hrs was also observed. In separate examinations, $Na^+-K^+$ ATPase activity in the extract of skeletal muscle was determined and enhanced significantly after 1 hr and 2 hrs. The results appear to indicate that the increased Pi in response to cold might be due to the higher activity of $Na^+-K^+$ ATPase. To clarify the regulatory mechanism of the enzyme, groups of fish were exposed to cold with 100 mM $Na{_2HAsO_4}$ for 1 hr and 2 hrs. The enhanced $Na^+-K^+$ ATPase activity was reduced significantly when treated with $Na{_2HAsO_4}$ compared to the control and also to the respective cold exposed fish. The experimental findings indicate that arsenic is involved in the regulation of cold induced $Na^+-K^+$ ATPase activity. The enhanced $Na^+-K^+$ ATPase activity in skeletal muscle of this species in cold and its inhibition by arsenic may play a critical role in environment.

Keywords:

toxicity, exposure, inorganic phosphorus, metabolism, potassium, skeletal muscle, water temperature, toxicology, Channa punctatus, adenosinetriphosphatase, arsenic, enzyme activity, sodium,

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