Bilal AKBULUT, Eyüp ÇAKMAK, Nilgün AKSUNGUR, Yahya ÇAVDAR

Effect of exposure duration on time to recovery from anaesthesia of clove oil in juvenile of russian sturgeon

Karaca mersinlerinde (Acipenser gueldenstaedtii) karanfil yağının anestetik etkisi iki çalışma ile ölçülmüştür. Doz tepkisi 0,22’den 0,90 g/L’ye kadar değişen dört farklı konsantrasyonda ölçülmüş ve anesteziye 5 ve 10 dakika maruz kalmanın anesteziden kurtulmaya etkisi incelenmiştir. Ortalama boyları 24,9±1,37 cm ve ağırlıkları 63,2±10,89 g olan karaca mersinleri karanfil yağı ile hazırlanan bayıltıcı solüsyona tek tek yerleştirilmiştir. Her balığın bayılma ( I1: hafif bayılma ve I2: tam bayılma) ve ayılma (R1: hafif ayılma ve R2: tam ayılma) süreleri saniye düzeyinde kayıt edilmiştir. Balıklar tam bayıldıktan sonra anestetik solüsyon içinde bekletilmiş ve ayılma süreleri kayıt edilmiştir. Karanfil yağına maruz kalan balıklar, 0,22 g/L hariç diğer dozlarda 3 dakikadan az sürede bayılarak dış uyarıya tepki vermemiş ve beş dakikadan az sürede ayılmışlardır. Anesteziye 5 ve 10 dakika maruz kalan balıklar 0,22, 0,45 ve 0,90 g/L dozlarda, 0,90 g/L doz hariç, 10 dakika içinde ayılmışlardır. Karanfil yağı dozu arttıkça bayılma süresi kısalmış ve ayılma süresi uzamıştır. Bayılma süresi ile doz arasında negatif r= -0,594 ve doz ile ayılma süresi arasında pozitif r=0,422 (P

Anahtar Kelimeler:

Acipenser gueldenstaedti, Syzygium aromaticum, anestezi, karanfil, maruz kalma, bitkisel yağlar, dozaj etkileri, Acipenser, anestetikler, vücut uzunluğu, vücut ağırlığı

Karaca mersinlerinin karanfil yağı ile bayıltılmasında anesteziye maruz kalma süresinin ayılmaya etkisi

The efficacy of clove oil as an anaesthetic was evaluated in Juvenile of Russian sturgeon (Acipenser gueldenstaedtii) under two experiments. Dose response examined using four anaesthetic concentrations varying from 0.22 to 0.90 g/L of clove oil and effect of exposure duration on the recovery time analysed for 5 and 10 min. Mean body length (cm), 24.9±1.37 and weight (g) 63.2±10.89, n=120, juvenile Russian sturgeon placed individually to the anaesthetic bath prepared with clove oil, and induction (I1: Sedation and I2: Loss of equilibrium) and recovery times (R1: Regain of equilibrium and R2: Sedation) recorded in seconds. Allowing fish remain in the anaesthesia after fish reaching anaesthesia stage, time to recovery from anaesthesia recorded. Fish exposed to clove oil failured to respond to external stimuli and reached to anaesthesia, sympocal stage in <3 min and recovered in <5 min at all doses except 0.22 g/L dose of clove oil. Exposure five and ten minute to anaesthesia at the 0.22, 0.45 and 0.90 g/L concentrations, fish recovered from anaesthesia in <10 min, except 0.90 g/L. The higher dose of clove oil resulted in significantly shorter induction time and prolonged recovery time. While correlation between induction time and dose determined as negative, r= -0.594 and a positive correlation has been found between dose and recovery time, r=0.422 (P<0.05). The study demonstrated that clove oil can be used as an effective anaesthetic measuring length and weight of juvenile Russian sturgeon and dose and exposure duration effect on time to recovery from anaesthesia of clove oil.

Keywords:

Acipenser gueldenstaedti, Syzygium aromaticum, anaesthesia, cloves, exposure, plant oils, dosage effects, Acipenser, anaesthetics, body length, body weight,

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