Tolga GÖKSAN, İlknur AK SİVRİKOZ, Cenker KILIÇ

Growth characteristics of the alga haematococcus pluvialis flotow as affected by nitrogen source, vitamin, light and aeration

Bu çalışmada, vejetatif Haematococcus pluvialis Flotow kültürlerinde çeşitli inorganik azot bileşikleri, ışık şiddetleri, havalandırma hızları ve özellikle vitaminlerin etkileri araştırıldı. Azot kaynakları arasında en iyi büyümeye NaNO3 (1,0 g/L) ve KNO3 (0,5 g/L)’da sırasıyla 25,3 ve 26,3x104 hücre/ml hücre yoğunlukları ile ulaşıldı. Vitamin denemelerinde, en yüksek hücre sayıları tiyamin, biyotin ve B12 için sırasıyla 0,1 (0,3 μM), 1,0 (4 μM) ve 0,1 mg/L (0,75 μM) konsantrasyonlarda bulundu. Ayrıca, vitamin karışımı yerine sadece tiyamin kullanımının yeterli olduğu görüldü. Hücre artışı, 20 ve 40 μmol foton m-2 sn-1’lik aydınlatma ile karşılaştırıldığında 75 ve 150 μmol foton m-2 sn-1’de önemli derecede daha yüksekti. Havalandırma için ise optimum üzeri şiddetler büyümeyi azaltıp, hücre çapı ve pigment içeriğini artırırken, optimum hava akış hızı 1 lpm olarak bulundu. Bu nedenle, optimum üzeri havalandırma yapılması, H. pluvialis hücrelerinin kırmızılaştırma safhasında ikincil bir stres faktörü olarak rol oynayabilir.

Anahtar Kelimeler:

azot, potasyum nitrat, sodyum nitrat, vitaminler, tiyamin, hava akımı, alg kültürü, biyotin, hava alma, pigmentler, kültür ortamı, ışık yoğunluğu, ışık, büyüme oranı, hücre büyümesi, retinol, stres faktörleri, B12 vitamini

Haematococcus pluvialis flotow alg kültürlerine ınorganik azot bileşikleri, ışık, havalandırma ve vitaminlerin etkisi

In the present study, the effects of various inorganic nitrogen sources, light intensities, aeration rates and especially vitamins were investigated in vegetative Haematococcus pluvialis Flotow cultures. The best growth among the nitrogen sources was achieved in NaNO3 (1.0 g/L) and KNO3 (0.5 g/L) with cell densities of 25.3 and 26.3x104 cells/ml respectively. In vitamin trials, the highest cell numbers were found in 0.1 (0.3 μM), 1.0 (4 μM) and 0.1 mg/L (0.75 μM) concentrations for thiamin, biotin and B12 respectively. It was also seen that the use of thiamin alone was sufficient instead of using the vitamin mix. Cell growth was significantly higher in 75 and 150 μmol photon m-2 s-1 illuminations compared to 20 and 40 μmol photon m-2 s-1. As for aeration, optimum airflow rate was 1.0 lpm, while supra-optimum levels ceased the growth, cell diameter and pigment content increased. So, it may play a role as a secondary stress factor on H. pluvialis cells in reddening stage.

Keywords:

nitrogen, potassium nitrate, sodium nitrate, vitamins, thiamin, air flow, algae culture, biotin, aeration, pigments, culture media, light intensity, light, growth rate, cell growth, retinol, stress factors, vitamin B12,

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