Taghi SEIFI, Mohammad Reza IMANPOOR, Amin GOLPOUR

The effect of different hormonal treatments on semen quality parameters in cultured and wild carp introduction

Bu çalışmanın amacı, ovaprim, insan koryonik gonadotropini (hCG) ve sazan hipofiz bezi (cPG) gibi farklı hormon uygulamalarının kültüre ortamında ve doğal sazan balıklarının spermatolojik parametrelerine ve seminal plazma kompozisyonuna etkilerini araştırmaktır. Dozaj testinde, motolite süresi, hareket edebilen spermatozoa yüzdesi, spermotokrit ve sperm hacmi hem uyarılmış hem de muamele görmüş gruplarda önemli (P

Anahtar Kelimeler:

spermatozoa, sazan balığı, kalsiyum, HCG, seminal plazma, sodyum, kolesterol, kültür balığı, glukoz, hormonal kontrol, magnezyum, hipofiz bezi, potasyum, semen, semen özellikleri, spermatosit

Doğal ve kültürü yapılan sazan balığının sperm kalitesi üzerine farklı hormonal uygulamaların etkileri

The objective of this study was to assay the effectiveness injection of different hormones such as ovaprim, human chorionic gonadotropin (hCG) and carp pituitary gland (cPG) on spermatological parameters and seminal plasma composition of cultured and wild carp. At the dosage tested, motility duration, percentage of motile spermatozoa, spermatocrit and sperm volume were significant changes (P<0.01) in both stimulated groups and treatments. Composition of seminal plasma $Na^+,k^+,C1^-$ glucose, cholesterol and protein) were significant changes in cultured and wild carp, but values of $Ca^{+2}$ and $Mg^{+2}$did not show significant difference (P<0.01) in treatments. In general, significant changes in all treatments for spermatological properties and composition of seminal plasma were observed. The results showed that ovaprim and carp pituitary gland hormones had more effective than human chorionic gonadotropin (hCG) in cultured and wild carp.

Keywords:

spermatozoa, carp, calcium, HCG, seminal plasma, sodium, cholesterol, fish culture, glucose, hormonal control, magnesium, pituitary, potassium, semen, semen characters, spermatocytes,

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Austriano, J.F., Mrco-Jimenez, F., Perez, L., Balasch, S., Garzon Penaranda, D.L., Vicente, D.S., Viudes-de- Castro, J.S. and Jover, M. 2006. Effect of HCG as spermiation inducer on European eel semen quality. Theriogenology, 66: 1012-1020. doi:10.1016/j.theriogenology.2006.02.041
Basavaraj, N. and Hedge, S.N. 2005. Some characteristics and short-term preservation of spermatozoa of Deccan mahseer (Tor kludree). Aquaculture Research, 36: 422-430. doi:10.1111/j.1365-2109.2004.01194.x
Brzuska, E. 2003. Artificial spawning of female Polish line 3 carp (Cyprinus carpio L.) after treatment with Pituitary homogenate and/or Ovopel. Aquaculture Research, 34: 1321-1327. doi:10.1046/j.1365-2109.2003.00944.x
Brzuska, E. and Adamek, J. 2008. Artificial spawning of African catfish Heterobranchus longifilis: differences between the effects on reproduction in females treated with carp pituitary homogenate or Ovopel. Aquaculture Research, 39: 96–102. doi:10.1111/j.1365-2109.2007.01880.x
Cacot, P., Eeckhoutte, P., Muon, D.T., Trieu, N.V., Legendre, M., Mariojouls, C. and Lazard, J. 2003. Induced spermiation and milt management in Pangasius bocourti (Sauvage, 1880). Aquaculture, 215: 67-77. doi:10.1016/S0044-8486(02)00032-7
Caille, N., Rodina, M., Kocour, M., Flajshanc, M. and Linhart, O. 2006. Quantity, motility and fertility of tench (Tinca tinca) sperm in relation to LHRH analogue and carp pituitary treatment. Aquaculture International, 14: 75–87. doi:10.1007/s10499-005-9015-0
Ciereszko, A. 2008. Fish Spermatology: Chemical composition of seminal plasma and its physiological relationship with sperm motility, fertilizing capacity and cryopreservation success in fish. Alpha Science Press, 546 pp.
Clearwater, S.J. and Crim, L.W. 1998. Gonadotropin releasing hormone-analogue treatment increases sperm motility, seminal plasma pH and sperm production in yellowtail flounder Pleuronectes ferrugineus. Journal of Fish Physiology and Biochemistry, 19: 349-357. doi:10.1023/A:1007759620936
Harmin, S.A. and Crim, L.W. 1993. Influence gonadotropic hormone releasing hormone analog (GnRHa) on sex steroid profile and milt production in male winter flounder Psedoupleronectes americanus. Journal of Fish Physiology and Biochemistry, 10: 399-407. doi:10.1007/BF00004506
King, H.R. and Young, G. 2001. Milt production by non spermiation male Atlantic salmon (Salmo salar) after injection of a commercial gonadotropin releasing hormone analoge preparation 17 a hydroxyprogesteron or 17a 20a dihydroxy-4- pregnan- 3-one alone or in combination. Aquaculture, 139: 179- 175. doi: 10.1016/S0044-8486(00)00472-5
Krol, J., Kowalski, R.K., Hliwa, A., Dietrich, G.J., Stabinski, R. and Ciereszko, A. 2009. The effects of commercial preparations containing two different GnRH analogues and dopamine antagonists on spermiation and sperm characteristics in the European smelt Osmerus eperlanus. Aquaculture, 286: 328-331. doi:10.1016/j.aquaculture.2008.09.034
Kucharczyk, D., Kuzawa, R., Luczynski, M., Glogawski, J., Babiak, I. and Wyszomirska, E. 1997. Induced spawning in bream, Abramis brama (L), using carp and bream pituitary extract and hCG. Aquaculture Research, 28: 139-144. doi:10.1046/j.1365-2109.1997.t01-1-00840.x
Lahnsteiner, F., Patzner, R.A. and Weismann, T. 1994. The testicular main duct and the spermatic duct in some cyprinid fishes. II. Composition of seminal fluid. Journal of Fish Biology, 44: 459-467. doi: 10.1111/j.1095-8649.1994.tb01226.x
Lahnsteiner, F., Berger, B., Patzner, R. and Weismann, T. 1998. Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters and spermatozoa metabolism. Aquaculture, 163: 163-181. doi:10.1016/S0044-8486(98)00243-9
Lim, H.K., Pankhurst, N.W. and Fitzgibbon, Q.P. 2004. Effects of slow release gonadotropin releasing hormone analog on milt characteristics and plasma levels of gonadal steroids in greenback flounder (Rhombosolea tapirina). Aquaculture, 240: 505-516. doi: 10.1016/j.aquaculture.2004.06.017
Linhart, O. and Billard, R. 1994. Spermiation and sperm quality of European catfish (Silurus glanis L.) after implantation of GnRH analogues and injection of carp pituitary extract. Journal of Applied Ichthyology, 10: 182-188. doi:10.1111/j.1439-0426.1994.tb00157.x
Linhart, O., Mims, S.D., Gomelsky, B. and Hiott, A.E. 2000. Spermiation of paddle fish (Polyodon spathula) stimulation with injection of LHRH analogue and carp pituitary extract. Aquatic Living Resource, 13: 1-6.
Linhart, O., Mims, S.D., Boris Gomelsky, B., Hiott, A.E., Shelton, W.L. and Cosson, J. 2003. Ionic composition and osmolality of paddlefish (Polyodon spathula, Acipenseriformes) seminal fluid. Aquaculture International, 11: 357-68. doi: 10.1023/A:1025773707439
Linhart, O., Rodina, M., Gela, D. and Kocour, M. 2004. Optimization of artificial propagation in European cat fish (Silurus glanis). Aquaculture, 235: 619–632. doi:10.1016/j.aquaculture.2003.11.031
Melamed, P., Gur, G., Elizur, A., Rosenfeld, H., Sivan, B., Rentier-Delrue, F. and Yaron, Z. 1996. Differential effects of gonadotropin-releasing hormone, dopamine and somatostatin and their second messengers on the mRNA levels of gonadotropin IIb subunit and growth hormone in teleost fish, tilapia. Neuroendocrinology, 64: 320-328. doi:10.1159/000127135
Miura, T., Yamauchi, K., Nagahama, Y. and Takahashi, H. 1991. Induction of spermatogenesis in male Japanese eel (Anguilla japonica) by a single injection of human chorionic gonadotropin. Zoological Science, 8: 63-73.
Morisawa, M. 1985. Initiation mechanism of sperm motility at spawning in teleost. Zoological Science, 2: 605- 615.
Mylonas, C.C., Gissis, A., Magnus, Y. and Zohar, Y. 1997a. Hormonal changes in male white bass (Morone chrysops) and evaluation of milt quality after treatment with a sustained-release GnRH delivery system. Aquaculture, 153: 301-313. doi: 10.1016/S0044-8486(97)00021-5
Mylonas, C.C, Scott, A.P., Vermeirssen, E.L.M. and Zohar, Y. 1997b. Changes in plasma gonadotropin II and sex steroid hormones, and sperm production of striped bass after treatment with controlled-release gonadotropin-releasing hormone agonist-delivery systems. Journal of Biology Reproduction, 57: 669- 675. doi:10.1095/biolreprod57.3.669
Ohta, H. and Tanaka, H. 1996. Relationship between serums levels of human chorionic gonadotropin (hCG) and 11-ketotestosterone after a single injection of hCG and induced maturity in the male Japanese eel, Anguilla japonica. Aquaculture, 153: 123-134. doi:10.1016/S0044-8486(97)00020-3
Pankhurst, N.W. 1994. Effects of gonadotropin releasing hormone analogue, human chorionic gonadotropin and gonadal steroids on milt volume in the New Zealand snapper Pagrus auratus (Sparidae). Aquaculture, 125:185-197. doi:10.1016/0044-8486(94)90295-X
Perez, L., Austriano, J.F., Tomas, A., Zegrari, S., Barrera, R., Espinos, F.J., Navarros, J.C. and Jover, M. 2000. Inducation of maturation and spermation in the male European eel: assessment of sperm quality throughout treatment. Journal of Fish Biology, 57: 1488-1504. doi:10.1111/j.1095-8649.2000.tb02227.x
Rottmann, R.W., Shireman, J.V. and Chapman, F.A. 1991. Determining sexual maturity of brood stock for induced spawning of fish. SRAC Publication, 423: 1-4.
Rurangwa, E., Kime, D.E., Olevier, F. and Nash, J.P. 2004. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234: 1-28. doi: 10.1016/j.aquaculture.2003.12.006
Shangguan, B. and Crim, L.W. 1999. Seasonal variation in sperm production and sperm quality in male winter flounder, Pleuronectes americanus: the effect of hypophysectomy, pituitary replacement therapy and GnRH-a treatment. Journal of Marine Biology, 137: 19-27. doi:10.1007/s002270050521
Shehadeh, Z.H., Madden, W.D. and Dohl, T.P. 1973. The effects of exogenous hormone treatment on spermiation and vitellogenesis in the grey mullet (Mulgil cephalus). Journal of Fish, 5: 479-487. doi:10.1111/j.1095-8649.1973.tb04477.x
Szabo, T., Medgyasszay, C. and Horvath, L. 2002. Ovulation induction in nase (Chondrosmoma nasus, Cyprinidae) using pituitary extract or GnRH analogue combined with domperidone. Aquaculture, 203: 389- 395. doi:10.1016/S0044-8486(01)00635-4
Vander Kraak, G., Suzuki, K., Itoh, H. and Kawauchi, H. 1992. Properties of common carp gonadotropin I and gonadotropin II. Gen Comp Endocrinol, 85: 217-229. doi:10.1016/0016-6480(92)90005-5
Verma, D.K., Routray, P., Nanda, P.K. and Sarangi, N. 2009. Seasonal variation in semen: characteristics and biochemical composition of seminal plasma of mrigal, Cirrhinus mrigala. Asian Fisheries Science, 22: 429- 443.
Vermeirssen, E.L.M., Mazorra de Quero, C., Shields, R.J., Norberg, B., Kime, D.E. and Scott, A.P. 2004. Fertility and motility of sperm from Atlantic halibut (Hippoglossus hippoglossus) in relation to dose and timing of gonadotropin-releasing hormone agonist implant. Aquaculture, 230: 547-567. doi: 10.1016/S0044-8486(03)00414-9
Yaron, Z. 1995. Endocrine control of gametogenesis and spawning induction in carp. Aquaculture, 129: 49-73. doi:10.1016/0044-8486(94)00229-H
Zheng, W. and Stacy, N.E. 1996. Two mechanisms for increasing milt volume in male gold fish (Carssius auratus). Journal of Experimental Zoology, 267: 287- 295. doi: 10.1002/(SICI)1097-010X(19961101)276:4 _____287::AID-JEZ6>3.0.CO;2-K
Zohar, Y. 1989. Fish reproduction, its physiology and artificial manipulation. In: M.C. Shilo and S.H Sargi (Eds.), Fish Culture in Warm Water Systems, Problems and Trends. CRC Press, 65-119.
Zohar, Y. and Mylonas, C.C. 2001. Endocrine manipulation of spawning induction in cultured fish from hormone to gene. Aquaculture, 197: 99-139. doi:10.1016/S0044-8486(01)00584-1