An experiment was conducted to monitor temporal changes in the gene expression of a specific molecular marker, HIF1α in response to a 15-day exposure to chronic hypoxia in a bottom dwelling carp, Cirrhinus mrigala. The expression level ofHIF-1α mRNA was increased in gills of C. mrigala in response to 0.5 mg/L, dissolved oxygen (DO) for 15 days with the timeof exposure, whereas it remained unchanged in fishes exposed to normoxia (DO, 6.5 mg/L). Based on the first experiment it isfound that HIF is expressed at moderate level 72 hours, and it peaks at 7 and 15 days, indicating that 72 h is a tolerable timelimit for survival under hypoxia for the particular species. Thus, a second experiment was conducted to evaluate the effect ofhypoxia (0.5 mg/L) for 72 h and assessed different parameters such as metabolic enzymes, antioxidant enzymes andhaematological parameters. The haematological parameters, such as RBC count, Hb and Hct values increased significantly(P
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