S. C. IHEANACHO, N. IKWO, N. O. IGWEZE, C. CHUKWUIDHA, E. O. OGUEJI, R. ONYENEKE

Effect of Different Dietary Inclusion Levels of Melon Seed (Citrullus lanatus) Peel on Growth, Haematology and Histology of Oroechromis niloticus Juvenile

High demand and expensive nature of conventional basal feed stuffs used in fish diet has led to search for alternativeand cheap basal feed sources. This study was carried out to evaluate the effect of melon seed peel on growth, haematologyand histology of Oroechromis niloticus. 150 Oroechromis niloticus juveniles (25.24±1.72g) were assigned to varying dietarylevels of melon seed peel (MSP) namely 0, 25, 50, 75 and 100% as diet treatments. Each treatment had ten fish per tank andwas triplicated. Fish were fed twice daily at 5% body weight for 56 days. Fish were weighed at the end of the experiment.Blood samples from each treatment were collected for haematological examination. Histology of fish intestine was examined.Data obtained were subjected to analysis of variance (ANOVA). Significant differences (p˂0.05) were observed in all thegrowth and haematological parameters. Treatment 5 had the best growth performance compared to other treatments andcontrol. PCV and Hb values were found to be highest in treatment 5 followed by control. Histological examination revealedthat 100% (treatment 5) dietary inclusion level of MSP had no deleterious effect on the fish while 25% dietary inclusionshowed mild changes in the structure of the villi found in the epithelial mucosa of fish intestine. 100 % dietary inclusionlevel of MSP is recommended in the diet of Oroechromis niloticus.

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AOAC (2000). Official Methods of Analysis of Balarin, J.D. and Haller R. D (1982): The Intensive culture of tilapia in thanks, receiving cases in recent advances in aquaculture. (Eds. J.F. Mur, R.K Roberts), London Croon Heln Ltd, 260-360.

Abaelu, A.M, Makinde, M.A., & Akinrimisi, E.O. (1979). Melon (egusi) seed protein 1: Study of amino acid composition of defatted meal. Nutrition Rep. International. 20: 605-613.

Abowei, J.F.N, & Ekubo, A.T. (2011). A Review of Conventional and Unconventional Feeds in Fish Nutrition, British Journal of Pharmacology and Toxicology, 2 (4): 179-191.

Agbebi, A.I., Llesanmi, W.O., Adegbeleye, D.O., Odulate, S.O, Obasa, A.I., & Olaoye, O.J. (2003). Growth and haematological effects of replacing Maize with feed wastes on the juvenile C. gariepinus (Burchell, 1822), Journal of Fisheries and Aquatic Science, 8: 535-543. https://www.scialert.net/abstract/?doi=jfas.2013.535. 543

Bahmani, M., Kazemi, R., & Donskaya, P., (2001). A comparative study of some haematological features in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiology and Biochemistry, 24: 135- 140. http://dx.doi.org/10.1023/A:1011911019155

Belelander ,G., & Ramaley, J.A. (1979). Essentials of Histology. C.V. Moeby Company, St. Louis Toronto, London

Chomicki, G., & Renner, S.S. (2014). Watermelon origin solved with molecular hylogenetics including Linnaean material: Another example of museomics, New Phytologist.205 (2): 526-32. http://dx.doi.org/10.1111/nph.13163

Dacie, J.V., & Lewis, S.M. (2011). Practical hematology. 11th edition, New York: Churchill Livingstone, pp 41.

El–Sayed, A.F.M., 2006. Tilapia culture. CAB International, Wallingford.

Erhunmwunse, N.O., & Ainerua, M.O. (2013). Characterization of some blood parameters of African Catfish (Clarias gariepinus), American-Eurasian Journal of Toxicological Sciences, 5(3):72-76. http://dx.doi.org/10.5829/idosi.aejts.2013.5.3.82159

Ezenwa, B.I.O., & Kusemiju, K., (1985). Seasonal changes in the gonads of the catfish, Chrysichythys nigrodigitatus (Lacepede) in Badagry Lagoon Nigeria. Biologia Africana, 2: 15 – 23.

Fagbenro, O.A. (1987). A review of biological and economical principles underlying commercial fish culture production in Nigeria. Journal of West African Fisheries 3: 171-177.

Fagbenro, O.A. (2004). Predator control of overpopulation in cultured tilapias and the alternative uses for stunted tilapias in Nigeria. Proceedings of the Sixth International Symposium on Tilapia in Aquaculture, pp.634-647. (R.B. Bolivar, G.C. Mair and K. Fitzsimmons, eds.). Manila, Philippines. September 12-16, 2004.

Falaye, A.E. (1992). Utilization of Agro-industrial wastes as fish feedstuffs in Nigeria. Proceeding of the 10th Annual Conference of FISON pp 47 – 57.

Food and Agriculture Organisation, (2010). Fisheries and Aquaculture Department Food and Agriculture Organization ofthe United Nations. The State of World Fisheries and Aquaculture.

Gabriel, U.U., Akinrotimi, O.A., Bekibele, D.O, Onunkwo, D.N, & Anyanwu, P.E., (2007). Locally produced fish feed, potentials for aquaculture development in subSaharan. African Journal of Agricultural Research 297; 287-295. http://dx.doi.org/10.5897/AJAR.

Haque, S., Pal, S., Mukherjee, A.K., & Ghosh, A.R. (2012). Histopathological and ultramicroscopic changes induced by Fluoride in soft tissue organs of the airbreathing teleost, Channa punctatus (Bloch), Research Report Fluoride 45(3) 263-273.

Houlihan, D., Bouiard, T., & Jobling, M. (2001). Food Intake in Fish. 2001 eds. Iowa State University Press. Blackwell Science Ltd. 418 pp.

Iheanacho, S.C., Ogunji, J.O., Ogueji, E.O., Nwuba, L.A., Nnatuanya, I.O., Ochang, S.N., Mbah, C.E., Usman, I.B., & Haruna, M. (2017). Comparative assessment of ampicillin antibiotic and ginger (Zingiber officinale) effects on growth, haematology and biochemical enzymes of Clarias gariepinus Juvenile. Journal of Pharmacognosy and Phytochemistry. 6(3): 761-767.

Ikwor, T.N., & Nwakpa, J.N. (2008). Floating capacity, growth and haematology of C. Gariepinus fingerlings fed different levels of melon peel diets. Abstract of World Aquaculture Society, 2016.

Kamisaka, Y., Fuji, Y., Yamamoto, S., Kurokawa, T., Ronnestad, I., Totland, G.K., Tagawa, M., & Tanaka, M. (2003). Distribution of CholecystokininImmunoreactive cells in the digestive track of the Larval teleost, ayu, Plecioglossue altivelis. General Comparative Endocrinology, 134, 116-121. http://dx.doi.org/10.1016/j.cbpb.200611.009

Micale, V., Campo, S., D\'Ascola, A., Guerrera, M.C., Levanti, M.B., Germanà, A., & Muglia, U., (2014). How many functions? Observations in seabreams. General and Comparative Endocrinology, 205, 166- 167. http://dx.doi.org/10.1371/journal.pone.0052428

Murashita, K., Kurokawa, T., Nilsen, T.O., & Ronnestead, I., (2009). Ghrelin, Cholecystokinin and peptide YY in Atlantic Salmon (Salmo salar) : Molecular cloning and tissue expression. General Comparative Endocrinology. 160, 223-235. http://dx.doi.org/10.1016/j.cbpb.200611.009

Nguyen, T., Davis, D.A., & Saoud, I.P. (2009). Evaluation of alternative protein sources to replace fish meal in practical diets for juvenile tilapia, Oreochromis spp. Retrieved from https://www.was.org.html

Nwanna, L.C.1, Falaye, A.E., Olarewaju, O.J., & Oludapo1,B.V. (2009). Evaluation of Nile Tilapia (Oreochromis niloticus L) fed dietary potato peels as replacement for yellow maize. FISON Conference proceedings:156-159

Obi, M. N., Kolo, R.J., & Orire, A.M. (2011). The production of floating fish feed using melon shell as a floating agent. International Journal of Science and Nature, 2 (3) 477-482. ISSN 2229-6441.

Ogunlade, I. (2007) Backyard Fish Farmers Information needs in Osun State, Nigeria. AAAE Conference proceedings (2007) 165-169

Orire, A.M, & Ricketts, O.A. (2013). Utilisation of Melon shell as dietary energy source in the diet of Nile Tilapia (Oreochromis niloticus) International Journal of Engineering and Science 2 (4) 05-11.

Sogbesan, A.O., & Ugwumba, A.A.A. (2008). Nutritional evaluation of termite (Macrotermes subhyalinus) meal as animal protein supplements in the diets of Heterobranchus longifilis (Valenciennes, 1840) fingerlings. Turkish Journal of Fisheries and Aquatic Sciences, 8, 149- 157.http://dx.doi.org/10.4194/13032712 v16_4_31

Steven C., & Louis, A. H. ( 2002). Understanding Fish Nutrition, Feeds, and Feeding.

Tsevis, A.A., & Azzaydi, T.A. (2000). Effect of feeding regime on selected species of fish. Article publication of FISON, Feb., 2000. Agora Site.

Webb, J.K.A., Khan, A., Nunez, B.S., Ronnestad, I., & Holt, G.J. (2010). Cholecystokinin: Molecular cloning and Immunohistochemical localization in the gastrointestinal tract of Larval Red drum, Sciaenopa ocellatus (L). General Comparative Endocrinology,166, 152-159. http://dx.doi.org/10.1016/j.ygcen.2009.10.010