Analysis of Genetic Diversity of White Shrimp (Metapenaeus affinis) from the Northwest of the Persian Gulf Using Microsatellite Markers

We investigated the genetic structure of Metapenaeus affinis population from the northern coasts (Khuzestan Province:Bahrakan and Lifeh-Boosiaf region) of the Persian Gulf, using five microsatellite markers. The samples of pleopods tissue ofshrimp were taken, DNA extracted and PCR was performed on microsatellite primers. The results revealed that all fivemicrosatellite loci were polymorphic, the number of alleles (Na) ranged from 5 to 12 per locus, and the observed and effectivenumber was 7 and 3.67, respectively, also the observed and expected heterozygosis was ranged from 0.1 to 0.77 and 0.1 and0.83, respectively, which indicated that the two populations of M. affinis possessed a rich genetic diversity. The deviationfrom Hardy–Weinberg equilibrium (P

___

Alarcon, J.A., Magoulas, A. & Alvarez, M.C. (2004). Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata). Aquaculture, 230, 65-80. http://dx.doi.org/10.1016/S0044-8486(03)00434-4

Antoro, S., Na-Nakorn, U. & Koedprang, W. (2005). Study of genetic diversity of orange-spotted grouper, Epinephelus coiodes, from Thailand and Indonesia using microsatellite markers. Marine Biotechnology, 8, 1-10. http://dx.doi.org/10.1007/s10126-005-5026-0

Baldwin, J.D. & Bass, A.L. (1998). Molecular phylogeny and biogeography of the marine shrimp penaeus. Molecular Phylogenetics and Evolution, 10, 399-407

Ball, A.O. & Chapman, R.W. (2003). Population genetic analysis of white shrimp, Litopenaeus setiferus, using microsatellite genetic markers. Molecular Ecology, 12, 2319-2330. http://dx.doi.org/ 10.1006/mpev.1998.0537

Bassam, B.J., Caetano-Anolles, G, & Gresshoff, P.M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, 196, 80-83. http://dx.doi.org/10.1016/0003-2697(91)90120-I

Beacham, T.D. & Macconachi, C. (2004). Microsatellite identification of individual sockeye salmon in Barkley Sound, British Colombia. Journal of Fish Biology, 61, 1021-1032. http://dx.doi.org/10.1111/j.1095-8649.2002.tb01860.x

Beadmore, J.A., Mair, G.C. & Lewis, R.I. (1997). Biodiversity in aquatic system in realation to aquaculture. Aquaculture Research, 28, 829-839. http://dx.doi.org/10.1046/j.1365-2109.1997.00947.x

Benzie, J.A.H. (2000). Population genetic structure in penaeid prawns. Aquaculture Research, 30, 95-119. http://dx.doi.org/10.1046/j.1365-2109.2000.00412.x

Brightte, J., Hansen, M. & Loeschcker, V. (2005). Microsatellite DNA analysis of northern pick (Esox locius L.) population: insights into the genetic structure and demographic history of a genetically depauperatee specious. Biological Journal of the Linnean Society, 814, 1-11. http://dx.doi.org/10.1371/journal.pone.0095436

Carvalho, G.R. & Hauser, L. (1994). Molecular genetics and the stock concept in fisheries. Reviews in Fish Biology and Fisheries, 4, 326-350. http://dx.doi.org/10.1007/978-94-011-1218-5_3

Cesari, M., Mularoni, L., Scanabissi, F., & Mantovani B. (2004). Characterization of dinucleotide microsatellite loci in the living fossil tadpole shrimp Triop cancriformis (Crustacea Branchiopoda Notostrace). Molecular Ecology Notes, 4, 733-735. http://dx.doi.org/10.1111/j.1471-8286.2004.00807.x

Espinosa, G., Jager, M., Garcia-Machado, E., Pichs, Y., Rodriguez, N.C., Barcia, A.R. & Deutsch, J. (2001). Microsatellites from the White Shrimp Litopenaeus schmitti (Crustacea, Decapoda). Biotecnología Aplicada, 18, 232- 234.

FAO, (2001). Tropical shrimp fisheries and their impact on living resources, Fisheries Circular, No. 974.

FAO, (1985). Fisheries and Aquacultue Information and Statiistics Service.10/06/1985.

Fe´ral, J.P. (2002). How useful are the genetic markers in attempts to understand and manage marine biodiversity? Journal of Experimental Marine Biology and Ecology, 268, 121-145 http://dx.doi.org/10.1016/S0022-0981(01)00382-3.

Ferraris, J.D. & Palumbi, S.R. (1996). Molecular Zoology. New York, Weily-Liss Inc Press., 580 pp.

Fevolden, S.E. & Pogson, G.H. (1997). Genetic divergence at the synaptophysin (Syp I) locus among Norwegian coastal and northeast Arctic populations of Atlantic cod. Journal of Fish Biology, 51, 895-908. http://dx.doi.org/10.1111/j.1095-8649.1997.tb01529.x

Fitzsimmons, N.N., Moritz, C. & Moore, S.S. (1995). Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution. Molecular Biology and Evolution, 12, 432-440. http://dx.doi.org/10.1093/oxfordjournals.molbev.a040 218

Francisco, A.K. & Galetti, P.M. Jr. (2005). Genetic distance between broodstock of the marine shrimp Litopenaeus vannamei (Decapoda, Penaeidae) by mtDNA analyses. Genetics and Molecular Biology, 28, 258- 261. http://dx.doi.org/10.1590/S1415- 47572005000200014

Gao, H., Kong, J., Yan, B., Yu, F., Luand, S. & Shengli, C. (2008). Permanent genetic resources: twelve new microsatellite markers for the Chinese shrimp Fenneropenaeus chinensis. Molecular Ecology Resources, 8, 325-327. http://dx.doi.org/10.1111/j.1471-8286.2007.01943.x

Garcia-Machado, E., Robainas, A., Espinosa, G., Oliva, M., Pa´ez, J., Verdecia, N. & Monnerot, M. (2001). Allozyme and mitochondrial DNA variation in Cuban populations of the shrimp Farfantepenaeus notialis (Crustacea: Decapoda). Marine Biology, 138, 701- 707. http://dx.doi.org/10.1007/s002270000475

Goyard, E., Arnaud, S., Vonau, V., Bishoff, V., Mouchel, O., Pham, D., Wyban, J. & Boudry, P. (2003). Residual genetic variability in domesticated populations of the Pacific blue shrimp (Litopenaeus stylirostris) of New Caledonia, French Polynesia and Hawaii and some management recommendations. Aquatic Living Resources, 16, 501-508. http://dx.doi.org/10.1016/j.aquliv.2003.07.001

Hartl, D.L., & Clark A.G. (1989). Principles of population genetics, Sinauer Associates in publisher, Sunderland, Massachusetts. USA, 565 pp.

Holthuis, L.B. (1980). FAO species catalogue Vol. 1: Shrimps and prawns of the world. An annotated catalogue of species of interest to fisheries, FAO Fish, Synop, 125, 271.

Klinbunga, S., Siludjai, D., Wudthijinda, W., Tassanakajon, A., Jarayabhand, P. & Menasveta, P. (2001). Genetic heterogeneity of giant tiger shrimp (Penaeus monodon) in Thailand revealed by RAPD and mitochondrial DNA RFLP analyses. Marine Biotechnology, 3, 428-438. http://dx.doi.org/10.1007/s10126-001-0055-9

Li, Z.X., Li, J., Wang, Q.Y., He, Y.Y. & Liu, P. (2006). The comparison of morphological characteristics in selected new variety “Huanghai No. 1” and the wild population of shrimp Fenneropenaeus chinensis. China Fishery Science, 13(3), 384-388.

Lima, A.P.S., Silva, S.M.B.C., Oliveira, K.K.C., Maggioni, R. & Coimbra, M.R.M. (2010). Genetics of two marine shrimp hatcheries of the Pacific white shrimp Litopenaeus vannamei (Boone, 1931), in Pernambuco, Brazil. Ciência Rural, 40, 325-331. http://dx.doi.org/10.1590/S0103-84782010005000008

Machado-Tamayo, R.J. (2006). Assessment of genetic variability in two lots of White shrimp, Litopenaeus vannamei (Boone, 1931) introduced to Cuba. Master in Science Thesis. Norwegian College of Fishery Science, University of Tromsø, Norway.

Mehanna, S.F., Al-Mamary, J., Al-Kharusi, L. (2012). Fishery characteristics and population dynamics of Indian white shrimp, Fenneropenaeus indicus from Arabian sea, Sultanate of Oman. Turkish Journal of Fisheries and Aquatic Sciences, 12, 239-246. doi: 10.4194/1303-2712-v12_2_07

Matheus, L.M. (2006). Variable microsatellite markers for a snapping shrimp (Alpheu armillatus) species complex. Molecular Ecology Notes, 7, 471- 473. http://dx.doi.org/10.1111/j.1471-8286.2006.01623.x

Meng, X.H., Wang, Q.Y., Jang, I.K., Liu, P. & Kong. J. (2009). Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA, Aquaculture, 287, 46-51. http://dx.doi.org/10.1016/j.aquaculture.2008.10.030

Moritz, C. (1994). Defining "evolutionary significant units" for conservation. Trends in Ecology and Evolution, 9, 373-375. http://dx.doi.org/10.1016/0169-5347(94)90057-4

Nash, J.H.E. (1991). DNA frag, program version 3.03. National Research Council of Canada, Ottawa, Ontario, Canada.

Nei, M. (1978). Estimation of average heterozygosity and genetic distance frome a small number of individuals. Genetics, 89, 583-590.

Norris, A.T., Breadley, D.G. & Cuningham, E.D. (1999). Microsatellite genetic variation between and with in farmed and wild Atlantic salmon population. Department of Genetics, Trinity College Dublin, Ireland, 247-264 pp.

Okumus, I., Ciftci, Y. (2003). Fish population genetics and molecular markers: II- molecular markers and their applications in fisheries and aquaculture. Turkish Journal of Fisheries and Aquatic Sciences, 3, 51-79.

Oliveira, E.J., Padua, J.G., Zucchi, M.I., Vencovsky, R. & Carneiro Vieira, M.L. (2006). Origin, evolution and genome distribution of microsatellites. Genetics and Molecular Biology, 29, 294-307. http://dx.doi.org/10.1590/S1415-47572006000200018

Peakall, R. & Smouse, P.E. (2006). GENALEX 6: genetic analysis in Excel, Population genetic software for teaching and research. Molecular Ecology Notes, 6, 288-295. http://dx.doi.org/10.1111/j.1471-8286.2005.01155.x

Raymond, M. & Rousset, F. (1995). GENEPOP: Population genetics software for exact test and ecumenicism. Journal of Heredity, 86, 248-249. http://dx.doi.org/10.1093/oxfordjournals.jhered.a1115 73

Rezaee, S., Farahmand, H. & Nematollahi, M.A. (2016). Genetic diversity status of Pacific white shrimp (Litopenaeus vannamei) using SSR markers in Iran. Aquaculture International, 24, 479-489. http://dx.doi.org/10.1007/s10499-015-9939-y

Rico, C., Ibrahim, K.M., Rico, I. & Hewitt, G.M. (1997). Stock composition in North Atlantic populations of whiting using microsatellite markers. Journal of Fish Biology, 51, 462-475. http://dx.doi.org/10.1111/j.1095- 8649.1997.tb01504.x.

Robainas, A., Monnerot, M., Solignac, M., Dennebouy, N. & Espinosa, G. (2002). Microsatellite loci from the pink shrimp Farfantenaeus notialis (Crustacea, Decapoda). Molecular Ecology Notes, 2, 344-345. http://dx.doi.org/10.1046/j.1471-8286.2002.00241.x

Robinson, J.P. & Hariss, S.A. (1999). Amplified fragment length Polymorphism and microsatellite: A Phylogenetic perspective. Amplified fragment length polymorphisms and microsatellites: A phylogenetic perspective. In: Gillet EM, ed. Which DNA marker for which purpose? Final compendium of the research project ‘Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees’ in the European Union DGXII Biotechnology FW IV research programme ‘Molecular tools for biodiversity’. http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarke r/index.htm.

Rosa-Velez, J de La., Escobar-Ferna´ndez, R., Correa, F., Maqueda-Cornejo, M. & Torre-Cueto, J de la. (2000). Genetic structure of two commercial penaeids (Penaeus californiensis and P. stylirostris) from the Gulf of California, as revealed by allozyme variation. Fishery Bulletin, 98, 674-683.

Slatkin, M. (1995). A measure of population subdivision based on microsatellite allele frequencies. Genetics, 139, 457-462.

Song, C., Li, J., Liu, P., Chen, P., & Gao, B. (2011). Microsatellite analysis of genetic diversity in 4 wild populations of Charybdis japonica. Journal of Fisheries of China, 35 (7), 985-991.

Sun, G. L., Diaz, O., Salomon, B., & Von Bothmer, R., (2001). Genetic diversity and structure in a natural Elymus caninus population from Denmark based on microsatellite and isozyme analyses. Plant Systematics and Evolution, 227, 235-244. http://dx.doi.org/10.1007/s006060170051

Supungul, P., Klinbunga, S., Pichyangkura, R., Jitrapakdee, S., Hirono, I., Aoki, T. & Tassanakajon, A. (2002). Identification of immune-related genes in hemocytes of black tiger shrimp (Penaeus monodon). Marine Biotechnology, 4, 487-494. http://dx.doi.org/10.1007/s10126-002-0043-8

Supungul, P., Sootanan, P., Klinbunga, S., Kamonrat, W., Jarayabhand, P. & Tassanakajon, A. (2000). Microsatellite polymorphism and the population structure of the Black Tiger Shrimp (Penaeus monodon) in Thailand. Marine Biotechnology, 2, 339- 347. http://dx.doi.org/10.1007/s101260000012

Thai, B.T., Pham, T.A. and Austin, G.M. (2006). Genetic diversity of common carp in Vietnam using direct sequencing and SSCP analysis of the mitochondrial DNA control region. Aquaculture, 258, 228-240. http://dx.doi.org/10.1016/j.aquaculture.2006.03.025

Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution, 24: 1596-1599. http://dx.doi.org/ 10.1093/molbev/msm092

Van Oosterhout, C., Hutchinson, W.F., Wills, D.P.M. & Shipley, P. (2004). Micro-Checker: software for identifying and correcting geno-typing errors in microsatellite data. Molecular Ecology Notes, 4, 535- 538. http://dx.doi.org/10.1111/j.1471-8286.2004.00684.x

Valles-Jimenez, R., Cruz, P. & Perez-Enriquez, R. (2005). Population genetic structure of Pacific white shrimp (Litopenaeus vannamei) from Mexico to Panama: microsatellite DNA variation. Marine Biotechnology, 6, 475-484. http://dx.doi.org/10.1007/s10126-004- 3138-6

Wright, S. (1978). Evolution and the genetics of populations variability within and among natural populations. University of Chicago Press, Chicago, IL, 80-89 pp.

Xu, Z., Primavera, J.H., Pena, L.D., Pettit, P., Belak, J. & Warren, A.A. (2001). Genetic diversity of wild and cultured Black Tiger Shrimp Penaeus monodon in the Philippines using microsatellites. Aquaculture, 199, 13-40. http://dx.doi.org/10.1016/S0044-8486(00)00535-4

Yeh, F.C., Yang, R.C. & Boyle, T. (1999). POPGENE version 1.3.1. Microsoft Window-bases Freeware for Population Genetic Analysis, Available: www.ualberta.ca/~fyeh/. University of Alberta and the Centre for International Forestry Research.

Zhang, K., Wang, W., Li, W., Zhang, Q., & Kong, J. (2014). Analysis of genetic diversity and differentiation of seven stocks of Litopenaeus vannamei using microsatellite markers. Journal of Ocean University of China, 13, 647-656. http://dx.doi.org/10.1007/s11802-014-2208-2

Zhang, T., Kong, J., Wang, W., & Wang, Q. (2010). Genetic variability assessed by microsatellites in the breeding populations of the shrimp Penaeus (Fenneropenaeus) chinensis in China. Aquaculture, 310, 229-233. http://dx.doi.org/10.1016/j.aquaculture.2010.07.025

Zhi-min, L., Li, X., Fu-liang, Y., & Guo-liang, C. (2010). SSR analysis of three species from primary parent and their first generation of Litopenaeus vannamei. Agricultural Biotechnology, 11, 57-61.
Turkish Journal of Fisheries and Aquatic Sciences-Cover
  • ISSN: 1303-2712
  • Başlangıç: 2015
  • Yayıncı: Su Ürünleri Merkez Araştırma Enstitüsü - Trabzon
Sayıdaki Diğer Makaleler

Effect of Different Dietary Inclusion Levels of Melon Seed (Citrullus lanatus) Peel on Growth, Haematology and Histology of Oroechromis niloticus Juvenile

S. C. IHEANACHO, N. IKWO, N. O. IGWEZE, C. CHUKWUIDHA, E. O. OGUEJI, R. ONYENEKE

Impact of Garlic and Curcumin on the Hepatic Histology and Cytochrome P450 Gene Expression of Aflatoxicosis Oreochromis niloticus Using RT-PCR

Manal I. EL BARBARY

Effect of Dietary Incorporation of Chemo-Attractants on Growth and Survival during Seed Rearing of Ompok bimaculatus (Bloch)

Priya Rawat Pradyut BISWAS, Arun Bhai PATEL, Himadri SAHA

Caligus cybii (Caligidae, Copepoda) Parasitising the Commercially Exploited Seer Fish, Scomberomorus commerson, from the Malabar Coast (India)- Occurrence and Adaptations

Ameri Kottarathil HELNA, Kappalli SUDHA, Panakkool Thamban ANEESH, Gopinathan ANILKUMAR

Species Identification Using DNA Barcoding on Processed Panga Catfish Products in Viet Nam Revealed Important Mislabeling

Tran Thi Thuy HA, Nguyen Thi HUONG, Nguyen Phuc HUNG, Yann GUIGUEN

Physiological and Molecular Responses of a Bottom Dwelling Carp, Cirrhinus mrigala to Short-Term Environmental Hypoxia

Tincy VARGHESE, Asim Kumar PAL, Puthiyottil MISHAL, Narottam Prasad SAHU, Subrata DASGUPTA

Analysis of Genetic Diversity of White Shrimp (Metapenaeus affinis) from the Northwest of the Persian Gulf Using Microsatellite Markers

Maryam SHOKOOHMAND, Hosseın ZOLGHARNEIN, Sakineh MASHJOOR, Faramarz LALOI, Ali Mohammad FOROUGHMAND, Ahmad SAVARI

Comparison of Catch and Survey Data for Assessing Northern Shrimp (Pandalus borealis) from Arnarfjordur (NW-Iceland) Using a Stock Production Model

Suman BARUA, Gudmundur THORDARSON, Ingibjorg G. JONSDOTTIR

Role of Parasitic Helminths in Bioremediating Some Heavy Metal Accumulation in the Tissues of Lethrinus mahsena

Amaal HASSAN, Samia MOHARRAM, Hanan EL HELALY

New Diatom Records for Turkish Freshwater Algal Flora from Lakes Ladik (Samsun, Turkey) and Hazar (Elazığ, Turkey)

Faruk MARAŞLIOĞLU, Elif Neyran SOYLU