Ana R. ALVAREZ SANCHEZ, Hector NOLASCO SORIA, Alberto PENA RODRIGUEZ, Humberto MEJIA RUIZ

In vitro Digestibility of Yarrowia lipolytica Yeast and Growth Performance in Whiteleg Shrimp Litopenaeus vannamei

Marine yeasts used in aquaculture disease control can also be an important protein source for improving feeding andnutrition of crustaceans. Yarrowia lipolyticca has been studied for its capacity to secrete heterologous proteins and highcontent of unsaturated fatty acids, beta-glucan, and mannane polymers in the cell wall. We measured in vitro digestibility of Y.lipolyticca by whiteleg shrimp Litopenaeus vannamei digestive enzymes, and an in vivo assay of Y. lipolytica in feed onwhiteleg shrimp growth. We found that digestive gland enzymes of shrimp digest Y. lipolytica, based on reduced opticaldensity of a yeast suspension. Digestion was –0.00236 ± 0.00010 OD U $min^{–1}$for intact cells and –0.00325 ± 0.00010 OD Umin–1for lysed cells. Release of reducing sugars in intact cells (5.3940 ± 0.1713 µmol $h^{–1}$), and lysed cells (0.8396 ± 0.2251µmol h–1) was measured. Digestive gland treatment significantly reduced cell viability (near 100%), relative to the control.Electron microscopy shows that the cell wall of Y. lipolytica exposed to the digestive gland enzymes was severely damaged.Shrimp diet containing Y. lipolytica resulted in significantly higher weight gain and specific growth rate of whiteleg shrimp.

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