Biochemical alterations in hoplobatrachus occipitalis exposed to sub lethal concentrations of cadmium

Yetişkin kurbağalara 28 gün boyunca 0.25, 0.50, 1.00 ve 2.00 mg/L kadmiyum muamelesi yapılmıştır. Karaciğerde kadmiyumun etkileri seçilen parametreler doğrultusunda (biyokimyasal parametreler, süperoksit dismutaz (SOD), katalaz (CAT), glutat (GSH) ve tiyobarbitürük asit reaktif maddeleri (TBARS) değerlendirilmiştir. Süperoksit dismutaz (SOD) ve katalaz (CAT)’ın spesifik aktivitesinde kontrole kıyasla doza bağlı önemli derecede (P

Anahtar Kelimeler:

antioksidanlar, kadmiyum, katalaz, lipit peroksidasyonu, karaciğer, süperoksit dismutaz, toksisite, toksikoloji, kan kimyası, enzim aktivitesi, glutatyon, ağır metaller

Yarı öldürücü konsantrasyonlarda kadminyum muamelesi yapılan hoplobatrachus occipitalis’da biyokimyasal değişimler

The adult crowned bullfrog, Hoplobatrachus occipitalis was exposed to 0.25, 0.50, 1.00 and 2.00 mg/L cadmium for 28 days. The effect of cadmium on selected biochemical parameters- superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) in the liver were tested. Biochemical observations revealed significant (P<0.05) dose-dependent increase in the specific activity of superoxide dismutase (SOD) and catalase (CAT) relative to controls. This could be due to increased production of these antioxidants to counteract oxidative stress and lipid peroxidation induced by cadmium. Glutathione (GSH) level decreased with increase in the concentration of heavy metal. Thiobarbituric acid reactive substances (TBARS) which is an index of lipid peroxidation increased as concentration of cadmium increased. The increased level of TBARS in the liver of cadmium exposed frogs is an indication of increased membrane lipid peroxidation which could lead to cell damage.

Keywords:

antioxidants, cadmium, catalase, lipid peroxidation, liver, superoxide dismutase, toxicity, toxicology, blood chemistry, enzyme activity, glutathione, heavy metals,

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