Effects of deltamethrin on lipase activity in guppies (Poecilia reticulata)

Yaygın kullanıma sahip bir sentetik pyrethroid olan deltamehtrinin Poecilia reticulata’daki lipaz enzim aktivitesi üzerine etkisi bu çalışma kapsamında incelenmiştir. Kontrol grubu ve 5 farklı deney grubu, deltamethrinin farklı konsantrasyonlarına maruz bırakılmışlardır. Deneyler 3’er defa tekrar edilmiş ve her bir deneyde 60 balık kullanılmıştır. Lipaz aktivitesi titrasyon yöntemi kullanılarak saptanmıştır. Kontrol grubunda lipaz aktivitesi 5 u/dak bulunurken, en yüksek konsantrasyonda deltamethrin verilen grupta 2,5 u/dak olarak bulunmuştur. Deney sonuçları deltamethrinin lipaz aktivitesini inhibe ettiğini göstermektedir. Lipaz aktivitesinde meydana gelen bu değişimin, balığın besin alımını ve fizyolojik şartlarını negatif olarak etkilemesi muhtemeldir.

Anahtar Kelimeler:

Poecilia reticulata, hayvan besleme, hayvan fizyolojisi, enzim inhibitörleri, piretroitler, toksisite, triasilgliserol lipaz, enzim aktivitesi, toksikoloji, deltametrin

Deltamethrinin lepistes (Poecilia reticulata)’deki lipaz enzimi üzerine etkisi

Effect of the deltamethrin, which is extensively used synthetic pyrethroid, was investigated on the lipase activity of Poecilia reticulata in the present study. One control and five experimental groups were exposed to different concentrations of deltamethrin. Three experiments, including 60 guppies each, were conducted. Titration method was used in order to determine the lipase activity. Lipase activity level in control group was 5 U/min, while it was 2.5 U/min in the highest concentration of deltamethrin exposed fish. These results revealed that deltamethrin inhibited the lipase activity which may have a negative effect on nutrition and physiological condition of fish.

Keywords:

Poecilia reticulata, animal nutrition, animal physiology, enzyme inhibitors, pyrethroids, toxicity, triacylglycerol lipase, enzyme activity, toxicology, deltamethrin,

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