Mojgan KHODADADİ, Aida ARAB, Amir JAFERİAN

A Preliminary Study on Sperm Morphology, Motility and Composition of Seminal Plasma of Shirbot, Barbus grypus

In the present study, we investigated some qualitative parameters of milt in an endemic cyprinid fish, Shirbot, Barbus grypus. The parameters including: sperm motility, sperm density and the composition of seminal plasma (calcium, sodium and potassium, phosphorous, cholesterol, glucose. In this regard, 13 males ready to spawning (TW: 2846 ± 324 g, TL: 65.8 ± 4.8 cm) were provided from the South Aquaculture Research Center. Then, fish were injected during 12 days intraperitoneally in one stage with 2 mg/kg.bw pituitary extract and after 10-12 h, the spermiation occurred and sperm collection was carried out. According to results, the mean values of assayed parameters were: sperm motility duration: 108±13.6 s; sperm density: 18.8±2.44; seminal plasma pH: 7.87±0.2; % spermatocrit: 76.07±9.57; glucose: 9.41±1.29 mg/dl; cholesterol: 25.71±2.99 mg/dl; total protein: 1.27±0.29 mg/dl; Na+: 93.73±7.78 mmol/l; K+: 44.3±4.5 mmol/l; Ca++: 0.86±0.06 ; Mg++: 1.43±0.18 mmol/l; total sperm length: 4.17±0.34 µm; sperm head length: 1.2±0.2 µm; sperm flagellum length: 3.01±0.21 µm. Also, significant correlation was found between sperm motility duration and pH of seminal plasma and also spermatocrit and sperm density

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Aas, G.H., Refstie, T. and Gjerde B. 1991. Evaluation of milt quality of Atlantic salmon. Aquaculture, 95: 125– 132.

Abdoli, A. 2000. The inlandwater fishes of Iran. Iranian Museum of Nature and Wildlife, Tehran. 378P (in Persian).

Alavi, S.M.H. and Cosson J. 2006. Sperm motility in fihes: (II) Effcts of ions and osmotic pressure. Cell Biology International, 30: 1–14. DOI: 10.1016/j.cellbi.2005.06.004

Alavi, S.M.H., Cosson, J. and Kazemi R. 2006. Semen characteristicsin Acipenser persicus in relation to sequential stripping. Journal of Applied Ichthyology 22 (Suppl. 1): 400–405. DOI: 10.1111/j.1439- 0426.2007.00994.x

Banaee, M. and Naderi, M. 2014.The reproductive biology of Shirbot (Barbus grypus Hackel, 1843) in the Maroon River, Iran. International Journal of Aquatic Biology, 2:43-52

Billard, R., Cosson, J. and Crime L. 1993. Motility of fresh and aged halibut sperm. Aquatic Living Resources, 6: 67–75. DOI: http://dx.doi.org/10.1051/alr:1993008

Billard, R., Cosson, J., Crim, L.W. and Suquet, M. 1995. Sperm physiology and quality. In: Bromage N.R., Roberts R.J. (eds.): Brood Stock Management and Egg and Larval Quality. Blackwell Science, Oxford, UK, 25–52.

Boitano, S. and Omoto, C.K. 1991. Membrane hyperpolarization activates trout sperm without an increase in intracellular pH. Journal of Cell Science, 98: 343–349.

Bozkurt, Y. and Öğretmen, F.2012. Sperm quality, egg size, fecundity and their relationships with fertilization rate of grass carp (Ctenopharyngodon idella). Iranian Journal of Fisheries Sciences, 11: 755-764

Bozkurt, Y., Secer, S., Bukan, N., Akcay, E. and Tekin N.2006 Relationship between body condition, physiological and biochemical parameters in brown trout (Salmo trutta fario) sperm. Pakistan Journal of Biological Science, 9:940-944. DOI: 10.3923/pjbs.2006.940.944

Ciereszko, A., Dabrowski, K.1993. Estimation of sperm density of rainbow trout, whitefih and yellow perch using a sperctrophotometric technique. Aquaculture, 109: 367–373. DOI: 10.1016/0044-8486(93)90175-X

Ciereszko, A., Glogowski, J. and Dabrowski K. 2000. Biochemical characteristics of seminal plasma and spermatozoa of freshwater fihes. In: Tiersch T.R., Mazik P.M. (eds.): World Aquaculture Society. Cryopreservation of Aquatic Species. Baton Rouge, LA, USA, 20–48.

Ingermann, R.L., Bencic, D.C. and Gloud J.G. 2002. Low seminal plasma buffering capacity corresponds to high pH sensitivity of sperm motility in salmonids. Fish Physiology and Biochemistry. 24: 299–307. DOI10.1023/A:1015037422720

Jamieson, B.G.M.1991. Fish evolution and systematics: Evidence from spermatozoa. Cambridge University Press. Cambridge. pp 319. DOI: 10.1046/j.1420- 9101.1992.5040721.x

Krasznai, Z., Marian, T., Izumi, H., Damjanovich, S., Balkay, L., Tron, L. and Morisawa M. (2000): Membrane hyperpolarization removes inactivation of Ca2+ channels leading to Ca2+ in Łux and initiation of sperm motility in the common carp. Biophysics, 97: 2052–2067. DOI: 10.1073/pnas.040558097

Krol, J., Glogowski, J., Demska-Zakes, K. and Hliwa P.2006. Quality of semen and histological analysis of testes in Eurasian perch Perca luviatilis L. during a spawning period. Czech Journal of Animal Science, 51,: 220–226.

Lahnsteiner, F., Patzner R.A. and Weismann T. 1993a. The testicular main duct and the spermatic duct in some cyprinid fihes. II. Composition of seminal flid. Journal of Fish Biology, 44: 459– 467.DOI: 10.1111/j.1095-8649.1994.tb01226.x

Lahnsteiner, F., Patzner R.A. and Weismann T.1993b. Energy resources of spermatozoa of the rainbow trout (Oncorhynchus mykiss) (Pices, teleostei). Reproduction Nutrition Development, 33: 349–360. DOI: 10.1051/rnd:19930404

Linhart, O., Billard, R. and Proteau, J.P.1994. Cryopreservation of European catfih (Silurus glanis L.) spermatozoa. Aquaculture, 115: 340–359. doi:10.1016/0044-8486(93)90148-R

Lahnsteiner, F., Berger, B., Weismann, T. and Patzner, R.A. 1996. Motility of spermatozoa of Alburnus alburnus (Cyprinidae) and its relationship to seminal plasma composition and sperm metabolism. Fish Physiologyand Biochemistry, 15: 167–179. DOI:10.1007/BF01875596

Lahnsteiner, F., Berger B., Weismann T. and Patzner R.A. 1998. Determination of semen quality of the rainbow trout by sperm motility, seminal plasma parameters and spermatozoal metabolism. Aquaculture, 163: 163–181. doi:10.1016/S0044-8486(98)00243-9

Lahnsteiner, F., Mansour, N. and Berger B. 2004. Seminal plasma proteins prolong the viability of rainbow trout (Oncorynchus mykiss) spermatozoa. Thriogenology, 62: 801–808. DOI: 10.1016/j.theriogenology.2003.12.001

Lahnsteiner, F. and Patzner, R.A. 2008. Fish spermatology: Sperm morphology and ultrastructure in fish. Alavi S.M.H., Cosson J., Coward K. and Rafiee G. (eds). Alpha Science International Ltd. Oxford. UK. pp 1- 61.

Morisawa, M., Hirano, T. and Suzuki K. 1979. Changes in blood and seminal plasma composition of the mature salmon (Oncorhynchus keta) during adaptation to freshwater. Comparative Biochemistry and Physiology, 64: 325–329. DOI: 10.1016/0300- 9629(79)90451-1

Morisawa, S. and Morisawa, M. 1986. Acquisition of potential for motility in rainbow trout and chum salmon. Journal of Experimental Biology, 126: 89– 96.

Morisawa, S. and Morisawa, M. 1988. Induction of potential for sperm motility by bicarbonate and pH in rainbow trout and chum salmon. Journal of Experimental Biology, 136: 13–22

Nikpei, M.1996. Research project report: biological study of Barbus grypus and Barbus sharpie. Iranian Fisheries Research Institute. 1: 52-64.

Öğretmen, F., Gölbaşi, S. and İnanan, B.E. 2014. Inhibitory effect of K+ and Ca2+ concentrations, pH, and osmolality of activation solution on motility of shabut (Barbus grypus Heckel 1843) spermatozoa. Turk Journal of Veterinary and Animal sciences. 38:245– 252.

Perez, L., Asturiano, J.F., Martinez, S., Tomas, A., Olivares, L., Moce, E., Lavara, R., Vicente, J.S. and Jover, M. 2003. Ionic com position and physiochemical parameters of the European eel (Anguilla anguilla) seminal plasma. Fish Physiology and Biochemistry, 28: 221–222. DOI: 10.1007/s10695-005-1553-x

Piironen, J., Hyvarinen, H. 1983. Composition of the milt of some teleost fishes. Journal of Fish Biology, 22: 351– 361. DOI: 10.1111/j.1095-8649.1983.tb04757.x

Rurangwa, E., Kime, D.E., Ollevier, F. and Nash J.P. (2004). The measurement of sperm motility and factors affecting sperm quality in cultured fih. Aquaculture, 234: 1–28. DOI: 10.1016/j.aquaculture.2003.12.006

Secer, S., Tekin, N., Bozkurt, Y., Bukan, N. and Akcay. 2004. Corrlation between biochemical and spermatological parameters in rainbow trout semen. I.J.A. 56(4); 274-28.

Stoss, J. 1983. Fish gamete preservation and spermatozoon physiology. In: Hoar W.S., Randall D.J., Donaldson E. (eds.): Fish Physiology. Academic Press, NY, USA, 9, 305–350

Suquet, M., Omnes, M.H. and Fauvel C.1982. Assessment of sperm quality in Turbut. Scophtalmus maximus. Aqualulture, 101: 177–185. doi:10.1016/0044- 8486(92)90241-C

Verma, D.K., Routray, P., Dash, C., Dasgupta, S. and Jena, G.K. 2009. Physical and biochemical characteristics of semen and ultrastructure of spermatozoa in six carp species. Turkish Journal of Fisheries and Aquatic Sciences, 9: 67–76.