Aleksandra DASKALOVA, Alexander PAVLOV, Ralica KYUCHUKOVA, Hristo DASKALOV

Humane Slaughter of Carp – A Comparison between Three Stunning Procedures

Fish welfare at slaughter is an area of considerable scientific and commercial importance during the last decade. The aim of this study was to evaluate the effect of three stunning procedures on stress and meat quality of common carp. The experimental fish were separated in three groups and stunned by percussion (Group PS), electricity (Group ES) or asphyxia (Group AS), followed by decapitation. Blood cortisol and glucose concentrations were measured as stress indicators, whereas drip loss was used as meat quality parameter. The lowest glucose concentration (3.86±1.26 mmol/L) and drip loss (2.02±0.42%) were observed in Group PS. On the contrary, Group AS showed the highest results (11.34±3.07 mmol/L and 3.22±0.75% for blood glucose and drip loss, respectively), whereas the electrically stunned carp took an intermediate position (6.05±1.29 mmol/L and 2.29±0.43%). We conclude that percussive stunning leads to considerable reduction of stress at slaughter and better meat quality (lower drip loss), whereas asphyxia is unacceptable method due to the strong stress reaction and increased drip loss. Electrical stunning can be an effective method for stunning carp, but requires improvements of the electrical device to eliminate the repeated current application

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Bahuaud, D., Mørkøre, T., Østbye, T.-K., Veiseth-Kent, E., Thomassen, M.S. and Ofstad, R. 2010. Muscle structure responses and lysosomal cathepsins B and L in farmed Atlantic salmon (Salmo salar L.) pre- and post-rigor fillets exposed to short and long-term crowding stress. Food Chemistry, 118: 602-615. Doi:10.1016/j.foodchem.2009.05.028

Barton, B.A. 2002. Stress in Fishes: A Diversity of Responses with Particular Reference to Changes in Circulating Corticosteroids. Integrative and Comparative Biology, 42: 517-525. Doi:10.1093/icb/42.3.517

Bertotto, D., Poltronieri, C., Radaelli, G., Negrato, E., Simontacchi, C., Poli, B.M. and Tibaldi, E. 2007. Welfare and quality of farmed trout fed high plant protein diets. 3. Alternative indicators to evaluate stress in fish. Italian Journal of Animal Science, 6: 789. Doi:10.4081/ijas.2007.1s.789

Bjørnevik, M. and Solbakken, V. 2010. Preslaughter stress and subsequent effect on flesh quality in farmed cod. Aquaculture Research, 41: 1-8. Doi:10.1111/j.1365-2109.2010.02498.x

Bonga, S.W. 1997. The stress response in fish. Physiological reviews, 77: 591-625. Braithwaite, V.A. and Ebbesson, L.O.E. 2014. Pain and stress responses in farmed fish. Rev. sci. tech. Off. int. Epiz., 33: 245-253.

Çağiltay, F., Atanasoff, A., Roydev, R., Zapryanova, D., Bozakova, N. and Ivanov, V. 2015. Comparison of effects of clove oil and electro aneshtesia on serum biochemical parameters in carp (Cyprinus carpio). SYLWAN.English Edition, 159: 449-460.

Council Regulation (EC) No 1099/2009 of 24 September 2009 on the protection of animals at the time of killing. Official Journal of the European Union, L303: 1-30.

Daskalova, A., Pavlov, A. and Daskalov, H. 2014. Contamination and persistence of Salmonella Enteritidis in stressed and unstressed common carp (Cyprinus carpio L.). Journal of Biology, 02: 32-38.

Daskalova, A.H. and Pavlov, A. 2015. Effect of two stunning methods on postmortem muscle pН and meat quality of common carp (Cyprinus carpio L.). Bulgarian Journal of Veterinary Medicine, 18: 83-90. doi:10.15547/bjvm.822

Daskalova, A.H., Bracke, M.B.M., Van de Vis, J.W., Roth, B., Reimert, H.G.M., Burggraaf, D. and Lambooij, E. 2016. Effectiveness of tail-first dry electrical stunning, followed by immersion in ice water as a slaughter (killing) procedure for turbot (Scophthalmus maximus) and common sole (Solea solea). Aquaculture, 455: 22-31. Doi:10.1016/j.aquaculture.2015.12.023

EFSA, 2009. Scientific Opinion of the Panel on Animal Health and Welfare on a request from the European Commission on Species-specific welfare aspects of the main systems of stunning and killing of farmed carp. The EFSA Journal, 1013: 1-37. http://www.efsa.europa.eu/sites/default/files/scientifi c_output/files/main_documents/1013.pdf (Accessed April 5, 2016).

Erikson, U., Lambooij, B., Digre, H., Reimert, H.G.M., Bondø, M. and Van der Vis, H. 2012. Conditions for instant electrical stunning of farmed Atlantic cod after de-watering, maintenance of unconsciousness, effects of stress, and fillet quality – A comparison with AQUI-S TM. Aquaculture, 324-325: 135-144. Doi:10.1016/j.aquaculture.2011.10.011

Fatira, E., Papandroulakis, N. and Pavlidis, M. 2014. Diel changes in plasma cortisol and effects of size and stress duration on the cortisol response in European sea bass (Dicentrarchus labrax). Fish physiology and biochemistry, 40: 911-919. Doi:10.1007/s10695-013- 9896-1

FAWC, 2014. Opinion on the welfare of farmed fish at the time of killing. https://www.gov.uk/government/uploads/system/uplo ads/attachment_data/file/319331/Opinion_on_the_we lfare_of_farmed_fish_at_the_time_of_killing.pdf. (Accessed April 5, 2016).

Huff-Lonergan, E. and Lonergan, S.M. 2005. Mechanisms of water-holding capacity of meat: The role of postmortem biochemical and structural changes. Meat Science, 71: 194-204. Doi:10.1016/j.meatsci.2005.04.022

Lambooij, E., Gerritzen, M.A., Reimert, H., Burggraaf, D. and Van de Vis, J.W. 2008. A humane protocol for electro-stunning and killing of Nile tilapia in fresh water. Aquaculture, 275: 88-95. Doi:10.1016/j.aquaculture.2008.01.026

Lambooij, E., Digre, H., Reimert, H.G.M., Aursand, I.G., Grimsmo, L. and Van de Vis, J.W. 2012. Effects of on-board storage and electrical stunning of wild cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) on brain and heart activity. Fisheries Reseach, 127– 128: 1-8. http://dx.doi.org/10.1016/j.fishres.2012.04.004

Lambooij, B., Digre, H., Erikson, U., Reimert, H., Burggraaf, D. and Van de Vis, H. 2013. Evaluation of Electrical Stunning of Atlantic Cod (Gadus morhua) and Turbot (Psetta maxima) in Seawater. Journal of Aquatic Food Product Technology, 22: 371-379. Doi:10.1080/10498850.2011.654047

Llonch, P., Lambooij, E., Reimert, H.G.M. and Van de Vis, J.W. 2012. Assessing effectiveness of electrical stunning and chilling in ice water of farmed yellowtail kingfish, common sole and pike-perch. Aquaculture, 364–365: 143–149. http://dx.doi.org/10.1016/j.aquaculture.2012.08.015

Martínez-Porchas, M., Martínez-Córdova, L.R. and Ramos-Enriquez, R. 2009. Cortisol and glucose: reliable indicators of fish stress. Pan-American Journal of Aquatic Sciences, 4: 158-178.

Moini, S., Mouloodi, Z., Shabanpour, B. and Rahmanifarah, K. 2011. Effects of stunning by ice and clove oil, and asphyxia on stress responses and quality indicators in common carp (Cyprinus carpio). Journal of Fisheries (Iranian Journal of Natural Resources), 64: 153-162.

Oliveira Filho, P.R.C., Oliveira, C.A.F., Sobral, P.J.A., Balieiro, J.C.C., Natori, M.M. and Viegas, E.M.M. 2015. How stunning methods affect the quality of Nile tilapia meat. CyTA-Journal of Food, 13: 56-62. Doi:10.1080/19476337.2014.911211

Olsson, G.B., Olsen, R.L. and Ofstad, R. 2003. Postmortem structural characteristics and water-holding capacity in Atlantic halibut muscle. LWT – Food Science and Technology, 36: 125-133. Doi:10.1016/S0023-6438(02)00205-0

Özogul, Y. and Özogul, F. 2004. Effects of slaughtering methods on sensory, chemical and microbiological quality of rainbow trout (Onchorynchus mykiss) stored in ice and MAP. European Food Research and Technology, 219: 211-216. Doi:10.1007/s00217-004- 0951-0

Pearce, K.L., Rosenvold, K., Andersen, H.J. and Hopkins, D.L. 2011. Water distribution and mobility in meat during the conversion of muscle to meat and ageing and the impacts on fresh meat quality attributes—A review. Meat Science, 89: 111-124. Doi:10.1016/j.meatsci.2011.04.007

Poli, B.M., Zampacavallo, G., Iurzan, F., de Francesco, M., Parisi, G. and Mosconi, G. 2002. Biochemical stress indicators changes in sea bass as influenced by the slaughter method. Proceeding of the ‘‘Aquaculture Europe 2002: Sea Farming Today and Tomorrow’’, 32, 429–430.

Poli, B.M. 2009. Farmed fish welfare-suffering assessment and impact on product quality. Italian Journal of Animal Science, 8: 139-160.

Robb, D.H.F, Callaghan, M.O., Lines, J.A. and Kestin, S.C. 2002. Electrical stunning of rainbow trout (Oncorhynchus mykiss): factors that affect stun duration. Aquaculture, 205: 359–371. Doi:10.1016/S0044-8486(01)00677-9

Rose, J.D., Arlinghaus, R., Cooke, S.J., Diggles, B.K., Sawynok, W., Stevens, E.D. and Wynne, C.D.L. 2014. Can fish really feel pain? Fish and Fisheries, 15: 97-133. Doi:10.1111/faf.12010

Roth, B., Moeller, D. and Slinde, E. 2004. Ability of Electric Field Strength, Frequency, and Current Duration to Stun Farmed Atlantic Salmon and Pollock and Relations to Observed Injuries Using Sinusoidal and Square Wave Alternating Current. North American Journal of Aquaculture, 66: 208- 216. Doi:10.1577/A03-022.1

Roth, B., Slinde, E. and Arildsen, J. 2006. Pre or post mortem muscle activity in Atlantic salmon (Salmo salar). The effect on rigor mortis and the physical properties of flesh. Aquaculture, 257: 504–510. Doi:10.1016/j.aquaculture.2005.10.021

Sattari, A., Lambooij, E., Sharifi, H., Abbink, W., Reimert, H. and Van de Vis, J.W. 2010. Industrial dry electrostunning followed by chilling and decapitation as a slaughter method in Claresse® (Heteroclarias sp.) and African catfish (Clarias gariepinus). Aquaculture, 302: 100-105. Doi:10.1016/j.aquaculture.2010.01.011

Sgoifo, A., De Boer, S.F., Haller, J. and Koolhaas, J.M. 1996. Individual differences in plasma catecholamine and corticosterone stress responses of wild-type rats: relationship with aggression. Physiology & Behavior, 60: 1403-1407. Doi:10.1016/S0031- 9384(96)00229-6

Sigholt, T., Erikson, U., Rustad, T., Johansen, S., Nordtvedt, T.S. and Seland, A. 1997. Handling Stress and Storage Temperature Affect Meat Quality of Farmed‐raised Atlantic Salmon (Salmo Salar). Journal of Food Science, 62: 898-905. Doi:10.1111/j.1365-2621.1997.tb15482.x

Toldrá, F. 2003. Muscle Foods: Water, Structure and Functionality. Food Science and Technology International, 9: 173-177. Doi:10.1177/1082013203035048

Van de Vis, H., Kestin, S., Robb, D., Oehlenschläger, J., Lambooij, B., Münkner, W., Kuhlmann, H., Kloosterboer, K., Tejada, M., Huidobro, A., Otterå, H., Roth, B., Sørensen, N.K., Akse, L., Byrne, H. and Nesvadba, P. 2003. Is humane slaughter of fish possible for industry? Aquaculture Research, 34: 211-220. Doi:10.1046/j.1365-2109.2003.00804.x

Varga, D., Szabó, A., Hancz, C., Jeney, Z., Ardó, L., Molnár, M. and Molnár, T. 2014. Impact of handling and pre-mortal stress on the quality of common carp (Cyprinus carpio L.). Israeli Journal of AquacultureBamidgeh, 66: 1-6.

Wilkinson, R.J., Paton, N. and Porter, M.J.R. 2008. The effects of pre-harvest stress and harvest method on the stress response, rigor onset, muscle pH and drip loss in barramundi (Lates calcarifer). Aquaculture, 282: 26 - 32. Doi:10.1016/j.aquaculture.2008.05.032

Woodward, C.C. and Strange, R.J. 1987. Physiological stress responses in wild and hatchery-reared rainbow trout. Transactions of the American Fisheries Society, 116: 574-579. Doi:10.1577/1548- 8659(1987)116<574:PSRIWA>2.0.CO;2