Maria MESSİNA, Joan Sanchez GURMACHES, Isabel NAVARRO, Joaquim GUTIERREZ, Daniela BERTOTTO, Emilio TIBALDI, Francesca TULL

Feeding frequency differently affects post prandial patterns of plasma glucose, insulin and insulin- like growth factor I in European sea bass (Dicentrarchus labrax)

The aim of this study was to investigate the effects of feeding frequency on post-prandial pattern of circulating glucose, insulin and IGF-I (insulin -like growth factor-I) in European sea bass (D. labrax). Fish (average body weight 72.1 ± 2 g) were randomly allocated in 12 tanks, reared in a closed thermo regulated recirculating system and fed a commercial diet at a fixed ration (1.5% body weight) for 7 weeks. Six fish groups were fed the ration in one meal a day (0 8 : 30 am), while six fish groups were offered the same ration in two daily meals (0 8 :30 am and 0 4 : 30 pm) according to a complete random design. Plasma insulin mean level was affected by the splitting up of the ration (14.4 vs 15.2 ng/ml, P<0.05, one meal and two meals/day, respectively) and showed a bimodal profile depending on the meal administration. The level of circulating glucose was slightly affected by feeding pattern with an increase of glycaemia after each me al. The daily pattern of plasma IGF-I did not appear related to meal administration. Results show that post prandial plasma glucose, insulin and IGF-I are differently affected by the meal administration pattern in sea bass due to their physiological role in maintaining the internal homeostasis.

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Andoh, T. 2007. Amino acids are more important insulin otropins than glucose in a teleost fish, barfin flounder (Veraspermoseri). General and Comparative Endocrinology, 151: 3 08 -317. doi: 10.1016/j.ygcen. 2007.01.015

Beckman, M., Shimizu, M., Gadberry, B. and Cooper, K.A. 20 04. Response of the somatotropic axis of juvenile coho salmon to alteration in plane of nutrition with an analysis of the relationship among growth rate an d circulating IGF-I and 41 kDa IGFBP. General and Comparative Endocrinology, 135: 334 -344. doi:10.1016/j.ygcen.2003.10.013

Duan, C. and Hirano, T. 1992. Effects of insulin -like growth factor-I and insulin on the in -vitro uptake of sulphate by eel branchia l cartilage:evidence for the presence of independent hepatic and pancreatic sulphation factors. Journal of Endocrinology, 133: 211 -219. doi:10.1677/joe.0.1330211

Duan, C. 1998. Nutritional and developmental regulation of insulin -like growth factors in fish. Journal of Nutrition, 128: 306 -314 .

Dyer, A.R., Upton, Z., Stone, D., Thomas, P.M., Soole, K.L., Higgs, N., Queen, K. and Carragher, J.F. 2004. Development and validation of a radioimmunoassay for fish insulin -like growth factor-I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels. General and Comparative Endocrinology , 135: 268 -275. doi:10.1016/j.ygcen. 2003.10.002

Ebbesson, L., Bjoernsson, B.T., Ekstroem, P. and Stefansson, S.O. 2008. Daily endocrine profiles in parr and smolt Atlantic salmon. Comparative Biochemistry and Physiology, 151: 698 -704. d oi: 10.1016 /j.cbpa.2008.08.017

Enes, P., Sanchez-Gurmaches, J., Navarro, I., Gutierrez, J. and Oliva-Teles, A. 2010. Role of insulin and IGF-I on the regulation of glucose m etabolism in European sea bass (Dicentrarchus labrax) fed with different dietary carbohydrate levels. Comparative Biochemistry and Physiology , 157: 346 -353. d oi: 10.1016/J.cbpa.2010.08.006

Enes, P., Panserat, S., Kaushik S. and Oliva-Teles A. 2011. Dietary carbohydrate utilization b y European Seabass (Dicentrarchus labrax L.) and gilthead sea bream (Sparus aurata L.) juveniles . Review in Fishery Science, 19 (3): 201 -215. d oi: 10.1080/10641262. 2011.579363

Fox, B.K., Breves, J.P., Hirano, T. and Grau, E.G. 2009. Effects of short- and long -term fasting on plasma and stomach ghrelin, and the growth hormone/insulin -like growth factor I axis in the tilapia, Oreochromis mossambicus . Domestic Animal Endocrinology, 37: 1 -11. doi: 10.1016/j.domaniend.2009.01.001

Froy, O. 2007. The relatioship between nutrition and circadian rhythms in mammals. Frontiers in Neuroendocrinology, 28: 61 -71. doi: 10.1016/j.yfrne. 2007.03.001

Gomez-Requeni, P., Mingarro, M., Calduch -Giner, J.A., Médale, F., Martin, S.A.M., Houlian, D.F. , Kaushik, S. and Pérez-Sánchez, J. 2004. Protein growth performance, aminoacid utilisation and somatotropic axis responsiveness to fish meal replacement by plant protein sources in gilthead sea bream (Sparus aurata ). Aquaculture, 232: 493 -510. doi:10.101 6/S00 44 -8486(03)00532 -5

Gouveia, A., Davies, S. J., 2004. Modulation of the Post‐Prandial Plasma Glucose Profile in Juvenile European Sea Bass Dicentrarchus labrax Fed Diets Varying in Starch Complexity. Journal of the World Aquaculture Society, 35 (3), 392 -400.

Gutiérrez, J., Carrillo, M., Zanuy, S., Planas, J., 1984. Daily rhythms o f insulin and glucose levels in the plasma of sea bass Dicentrarchus labrax after experimental feeding. General and Comparative Endocrinology, 55(3), 393 -397.

La Fleur, S.E. 1999. A Suprachiasmatic Nucleus Generated Rhythm in Basal Glucose Concentrations. Journal of Neuroendocrinology, 11: 643 -652.

Mahon, R., Khokiattiwong, S., Oxenford, H. A. 2000. Selectivity of experimental gillnets for fourwing flyingfish, Hirundichthys affinis, off Barbados. Environmental Biology of Fishes, 59(4), 459 -463.

Mineo, H., O yamada, T. and Kato, S. 1990 . Effect of feeding on plasma secretin concentrations in sheep. Research in Veterinary Scienc e, 49(2): 157 -159.

Mitchell, G.A. 1990. Method of s tarch a nalysis . Starch, 42(4): 132 -134.

Mommsen, T.P. and Plisetskaya, E.M., 1991. Insulin in fish and agnathans: history, structure and metabolic regulation. Review in Aquatic Science, 4: 225 -259.

Mommsen, T.P. 1998. Growth and metabolism. In: D.H . Evans (Ed.), The Physiology of Fishes. Second Ed it., CRC Press, Boca Raton: 65 -97.

Mommsen, T.P., Moon, T.W. and Plisetskaya, E.M. 2001. Effect of arginine on pancreatic hormone and hepatic metabolism in rainbo w trout. Physiological and Biochemical Zoology, 74: 668 -678. doi: 10.1086/32 2924

Moriyama, S., Ayson, F.G. and Kawauchi, H. 2000. Growth Regulation by Insulin -like Growth Factor-I in Fish. Bioscience Biotechnology and Biochemistry, 64(8): 1553 -1562. doi: 10.1271/bbb.64.1553

Navarro, I., Carneiro, M., Párrizas, M., Maestro, J.L., Planas, J. and Gutierrez, J. 1993. Post-feeding levels of insulin and glucagon in trout (Salmo trutta fario ). Comparative Biochemistry and Physiology, 104: 389 - 393. doi: 10.1016 /0300 -9629(93)90335 -2

Navarro, I. and Gutierrez, J. 1995. Fasting and starvation. In: P.W. Hochacka, T.P. Mommsen (Eds.), Metabolic Biochemistry . Elsevier Science BV, Amsterdam: 393 - 434.

Niu, P., Pérez-Sanchez, J. and Le Bail, P.1993. Development of a protein binding assay for teleost insulin -like growth factor (IGF)-like: relationship between growth hormone (GH) and IGF-like in the blood of rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 11: 381 -391.

Papatryphon, E., Capilla, E., Nav arro, I. and Soares, H.J. Jr. 2001. Early insulin and glucagon response associated with food intake in a teleost, the striped bass (Morones axatilis). Fish Physiol. and Biochemistry, 24: 31 -39. doi: 10.1023/A:1011123908446

Perez, J., Zanuy, S. and Carrillo, M. 1988. Effects of diet and feeding times on daily variations in plasma insulin, hepatic c -AMP and other metabolites in a teleost fish Dicentrarchus labrax L. Fish Physiology and Biochemistry, 5: 191 -197. doi: 10.1007/BF01874796

Pérez -Sanchez, J., Martí-Palanca, H. and Le Bayl, P.J. 1994. Seasonal changes in circulating growth hormone (GH), hepatic GH -binding and plasma insulin -like growth Factor-I immunoreactivity in a marine fish, gilthead sea bream, Sparus aurata . Fish Physiology and Biochemistry, 1 3: 199 -208 . doi: 10.1007/BF0000 4358

Pérez -Sanchez, J., Martí-Palanca, H. and Kaushik, S. 1995. Ration size and protein intake affect circulating growth hormone concentration, hepatic growth hormone binding and plasma insulin -like growth factor I immunoreactivity in a marine fish, gilthead sea bream, Sparus aurata . Journal of Nutrition, 125: 546 -552.

Pierce, A.L., Shimizu, M., Beckman, B.R., Backer, D.M. and Dickhoff, W.W. 2005. Time course of the GH/IGF-I axis response to fasting and increased ration in chinook salmon (Oncorhynchus tshawytscha ). General and Comp . Endocrinology, 140: 192 -202. doi: 10.1016/j.ygcen.2004.10.017

Plisetskaya, E.M., Buchelli-Narvaez, L.I., Hardy, R.W., Dickhoff, W.W. 1991. Effects of injected and dietary arginine on plasma insu lin levels and growth of Pacific salmon and rainbow trout. Comparative Biochemistry and Physiology, 98: 165 -170. doi:10.1016/0300 -9629(91)90595 -4

Salacinski, P.R.P., McLean, C., Sykes, J.E.,Clement-Jones, V.V .,Lowry, P.J . 1981. Iodination of proteins, glycop roteins, and peptides using a solid -phase oxidizing agent, 1,3,4,6 -tetrachloro -3a,6a-diphenyl- glycoluril (IODO -GEN). Analytical. Biochemistry, 117: 136 -146. doi: 10.1016/0003 -2697(81)90703 -X

Shimizu, M., Swanson, P., Fukada, H., Hara, A. and Dickhoff, W.W. 2000. Comparison of extraction methods and assay validation for salmon insulin -like growth Factor-I using commercially available components . General and Comp . Endocrinology, 119: 26 -36. doi: 10.1006/gcen.2000.7498

Shimizu, M., Cooper, K.A., Dickhoff, W. W. and Beckman, B.R. 2009. Postprandial changes in plasma growth hormone, insulin, insulin -like growth factor one (IGF- I), and IGF-binding proteins in coho salmon fasted for varying periods. American Journal of Physiology Regulatory Integrative and Compara tive Physiology, 297: 352 -361. doi: 10.1152/ajpregu.90939.2008

Silverstein, J.T., Wolters, W.R., Shimizu, M. and Dickhoff, W.W. 2000. Bovine growth hormone treatment of channel catfish: strain and temperature effects on growth, plasma IGF-I levels, feed intake and efficiency and body composition. Aquaculture, 190: 77 -88. doi: 10.1016/S0044 -8486(00)00387 -2

Sing, A.K., Lal, B. and Pati, A.K. 2009. Variability in the characteristic of ultradian and circadian rhythms in plasma levels of growth hormone in the Indian walking catfish, Clarias batrachus. Biological Rhythm Research,40: 211 -22. doi: 10.1080/09291010701875054

Small, B.C. 2005. Effect of fasting on nychthemeral concentrations of plasma growth hormone (GH), insulin -like growth factor I (IGF-I), and c ortisol in channel catfish (Ictalurus punctatus ). Comp. Biochem. and Physiol., 142 (B): 217 -223. doi: 10.1016/j.cbpb.2005.07.008

Towhidi, A., Zali, A., Khoshsokhan, A., Khazali, H., Zhandi, M., Rezayazdi, K., Masoumi, R. and Gholami, H. 2010. The effect of feeding frequency on the hormonal profile, carcass characteristics, and feedlot performance in Iranian Holstein calves . Tu rkish Journal of Veterinary and Animal Sciences, 34(2): 137 -142. doi: 10.3906/vet-0712 -5

Vega-Rubin de Celis, S., Gòmez -Requeni, P. and Pérez- Sànchez, J. 2004. Production and characterization of recombinantly derived peptides and antibodies for accurate d eterminations of somatolactin, growth hormone and insulin -like growth factor-I in European sea bass (Dicentrarchus labrax). General and Comparative Endocrinology, 139: 266 -277. doi:10.1016/j.ygcen.2004.09.017

Vicari, T., van den Borne, J.G.C., Gerrits, W.J .J., Zbinden, Y . and Blum, J.W. 2008. Postprandial blood hormone and metabolite concen trations influenced by feeding frequency and feeding level in veal calves . Domestic Animal Endocrinology, 34(1):74 -88. doi: 10.1016/j.domaniend.2006.11.002