E. LUSZCZEK TROJNAR, Sroka K., Klaczak A, Nowak M, W. POPEK

Bioaccumulation and purification of cadmium in Tubifex tubifex

This study compared accumulation of cadmium (Cd) in Tubifex worms (Tubifex tubifex) during exposure to different doses of the metal (0.9 and 2.5 mg/kg) in bo ttom sediments and in water (0.9 and 2.5 mg/l). Elimination of Cd from these invertebrates by prior exposure was also examined. Cd concentration in water, sediments and worms was analysed by Atomic Absorption Spectroscopy (AAS) method. The results showed higher sensitivity of Tubifex worms to Cd concentration in water (Concentration factors from 16 to 60) than bottom sediments (CF from 0.44 to 0.77). Cd bioaccumulation in worms was positively correlated with its dose and exposure duration (0.88 to 0.94). The highest level of Cd in worms (150 mg/kg) was observed after two days of exposure in water to 2.5 mg Cd/L. The results of purification that was studied the first time after the previous exposure to Cd showed th at Tubifex tubifex rapidly reduced Cd concen tration (from 28.5 to 0.13 mg/kg) in 3 days after exposure cessation. The results indicate that it is possible to purify Tubifex worms taken from a population existing in contaminated environment to obtain safe food for aquarium fish. Additionally, Tubife x worms can be considered as an ideal cumulative bioindicator of Cd in the water environment.

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Agemian, H., Sturtevant, D.P., Austen, K.D. 1980. Simultaneous acid extraction of six trace metals from fish tissue by hot-block digestion and determination by atomic -absorption spectrometry. Analyst, 1 05(1247): 125 130. doi: 10.1039/AN9800500125

Ali MHH, Fishar MRA. 2005. Accumulation of trace metals in some benthic invertebrate and fish species relevant to their concentration in water and sediment of Lake Qarun, Egypt. Egyptian Journal of Aquatic Resea rch, 31(1): 289 -301. http://hdl.handle.net/1834/1215

Arendarczyk A., Jakubowska A., Zgórska A., Grabińska - Sota E. 2014. Toxic effects of cadmium -spiked sediments in Tubifex tubifex: enzyme biomarkers measurements. Desalination and Water Treatment, 52(19 -21 ): 3798 -3803. doi: 10.1080/19443994.2014. 884793

Back H. 1990. Epidermal uptake of Pb, Cd and Zn in tubificid worms. Oecologia, 85: 2 26 -232. doi: 10.1007/BF00319405

Bailey H.C., Liu D.H.W. 1980. Lumbriculus variegates , a benthic oligochaete, as a bioassay organism. In J.C. Eaton, P.R. Parrish and A.C. Hendricks (Eds), Aquatic Toxicology ASTM STP 707, Philadelphia, 205 -215.

Biagianti-Risbourg S., Bastide J. 1995. Hepatic perturbations induced by a herbicide (atrazine) in juvenile grey mullet Liza ramada (Mu gilidae, Teleostei): An ultrastructural study. Aquatic Toxicology, 31: 217 -229. doi: 10.1016/0166 - 445X(94)00065 -X

Bouche ML., Habets F., Biagianti-Risbourg S., Vernet G. 2000. Toxic effects and bioaccumulation of cadmium in the aquatic Oligochaete Tubifex tubifex. Ecotoxicology and Environmental Safety , 46: 246 - 251. doi: 10.1006/eesa.2000.1919

Brkovic -Popovic I., Popovic M. 1977. Effects of heavy metals on survival and respiration rate of tubificid worms. Part I: Effects on survival. Environmental Pollution 13: 65 -72. doi: 10.1016/0013 - 9327(77)90093 -3

Bryan G.W., Hummerstone L.G. 1971. Adaptation of the polychaete Nereis diversicolor to estuarine sediments containing high concentrations of heavy metals. I. General observations and adaptation to copper. Assoc iation of the United Kingom. Journal of the Marine Biological Association of the United Kingdom , 51: 845 -863. doi: 10.1017/S0025315400018014

Chapman P. M., Farrell M. A., Brinkhurst R. O. 1982. Relative tolerances of selected aquatic oligochaetes to individual pollutants and environmental factors. Aquatic Toxicology 2: 47 -67. doi: 10.1016/0166 - 445X(82)9000 5 -4

Dallinger R. 1994. Invertebrate organisms as biological indicators of heavy metal pollution. Applied Biochemistry and Biotechnology, 48: 27 -31. doi: 10.1007/BF02825356

Depledge M.H., Weeks J.M., Bjerregaard P. 1994. Heavy metals. In P. Calow (Ed) Handbook of Ecotoxicology. Second edition, Blackwell Scientific, Oxford, 79 -105.

Dhainaut-Curtois N., Arrouijal F. Z., Demuynek S. 1988. Effets biologiques de trois metaux lourds (chrome, nickel et plomb) sur Nereis diversicolor (Annelide polychete). Oceanis, 14: 423 -433.

Dhainaut-Curtois N., Demuynek S., Salzet-Reveillon B. 1991. Mecanismes de detoxication chez les poissons et invertebres marins. Oceanis, 17, 403 -419.

Eisler R. 1981. Trace metal concentrations in marine organisms. Pergamon Press, Oxford.

Fargasova A. 1994. Toxicity of metals on Daphnia and Tubifex tubifex. Ecotoxicology and Environmental Safety, 27: 210 -213. doi: 10.1006/eesa.1994.1017

Hall L.W.Jr., Scott M.C., Killen W.D. 1988. Ecological risk assessment of copper and cadmium in surface waters of Chs apeake Bay watershed. Environmental Toxicology and Chemistry, 17: 1172 -11 89. doi: 10.1002/etc.5620170626

Journal of Laws 2002. Sorts and concentrations of substances which cause that the spoil is contaminated. Regulation of the Minister of the Environment No 55 item 498 (accessed 16 April 2002).

Journal of Laws 2011. Regulation of the Minister of Environment on the classification of the status of surface waters and environmental quality standards for priority substances. No 257 item. 1545, (accesed 9 Novem ber 2011)

Kaonga C.C., Kumwenda J., Mapoma H.T. 2010. Accumulation of lead, cadmium, manganese, copper and zinc by sludge worms: Tubifex tubifex in sewage sludge. International Journal of Environmental S cience & Technology , 7(1): 1 19 -126. doi: 10.1007/BF03326123

Khangarot B. S. 1991. Toxicity of metals to a freshwater tubificid worm, Tubifex tubifex (Muller). Bulletin of Environmental Contamination and Toxicology, 46: 906 -912. doi: 10.1007/BF01689737

Luc an - Bouche M., Biagianti-Risbourg S., Arsac F., Vernet G. 1999. An original decontamination process developed by the aquatic oligochaete Tubifex tubifex exposed to copper and lead. Aquatic Toxicology, 45: 9 -17. doi: 10.1016/S0166 -445X(98)0009 1 -5

Meller M., Egeler P., Römbke J., Schallnass H., Nagel R., Streit B. 1998. Short-term toxicity of lindane, hexachlorobenzene, and copper sulfate to tubificid sludgeworms (Oligochaeta) in artificial media. Ecotoxicology and Environmental Safety, 39: 10 20. doi: 10.10 06/eesa.1997.1603

Milani D., Reynoldsom TB, Bormann U., Kolasa J. 2003. The relative sensitivity of four benthic invertebrates to metals in spiker-sediment exposures and application to contaminated field sediment. Environmental Toxicology and Chemistry, 22 : 845 -8 54. doi: 10.1002/etc.5620220424

Pesh C. E., Hoffman G. 1982. Adaptation of the polychaete Neanthes arenaceodentata to copper. Marine Environmental Research, 6: 307 -317. doi: 10.1016/0141 -1136(82)90044 -7

Reinecke S.A., Prinsloo M.W., Reinecke A.J. 19 99. Resistance of Eisenia fetida (Oligochaeta) to cadmium after a long -term exposure. Ecotoxicology and Environmental Safety, 42: 75 -80. doi: 10.1006/eesa.1998.1731

Renfro W.C. 1983. Transfer of 65Zn from Sediments by Marine Polycheate Worm. Marine Biology , 21: 305 - 316. doi:10.1007/BF00381087

Reynoldson T.B., Rodriguez P., Madrid M.M. 1996. A comparison of reproduction, growth, and acute toxicity in two populations of Tubifex (Muller, 1774) from the North American Great Lakes and Northern Spain. Hydrobiolo gia, 334: 1 99 -206. doi: 10.1007/BF00017370

Spencer K.L., MacLeod C.L. 2002. Distribution and Partitioning of Heavy Metals in Estuarine Sediment Cores and Implications for the Use of Sediment Quality Standards. Hydrology and Earth System Sciences, 6(6): 989 -998. doi: 6/989/2002/hess -6 -989 - 2002

Whitley L. S. 1967. The resistance of tubificid worms to three common pollutants. Hydrobiologia 32: 1 93 -205. doi: 10.1007/BF00179550