Hossein KHARA, Mahmoud SAYYADBORANİ, Mohammad SAYYADBORANİ

Effects of ?-Tocopherol (vitamin E) and Ascorbic Acid (Vitamin C) and Their Combination on Growth, Survival and Some Haematological and Immunological Parameters of Caspian Brown Trout, Salmo Trutta Caspius juveniles

We examined the effects of different dietary levels of ascorbic acid (vitamin C) and ?- tocopherol (vitamin E) and their combinations on growth, survival; and some haematological and immunological parameters of Caspian brown trout, Salmo trutta caspius juveniles. The experiment was designed as 15 experimental treatments and one control group. 480 fish (35±5.5 g) were fed by experimental diets containing different levels of Vit C and E and control diet separately for a period of 2 months. According to the results, Vit C and E improved survival and growth parameters including specific growth rate (SGR), percent weight gain (WG) and biomass. The highest values of these parameters obtained in T8 (Vit E (30 mg.kg-1) + Vit C -1) and T9 (Vit E (40 mg.kg-1) + Vit C (300 mg.kg-1). The lowest FCR obtained in T8. The haematological parameters including red blood cells (RBCs), white blood cells (WBCs), haematocrit (%Hct) and haemoglobin (Hb) were higher in vitamin treated groups than control group with highest values in T8. In T13 (Vit E (20 mg.kg-1), WBC values were higher compared to other experimental groups. The immunological parameters (lysozyme activity, Immunoglobulin (IgM) and total immunoglobulin (TIg)) were found to be higher in vitamin supplemented groups than in control group with highest values in T12 (Vit C (300 mg.kg-1). In conclusion, our results show that a combination of 30 mg.kg diet -1 Vit E+ 300 mg.kg-1 Vit C or 40 mg.kgVit E + 300 mg.kg-1 Vit C could be a good option for obtaining appropriate growth and survival in Caspian brown trout juveniles.Caspian brown trout.

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