This research aimed to provide a characterization of fresh semen in wild and threatened Mediterranean brown trout (Salmo cettii), inhabiting the Biferno River basin and to study the variance of sperm parameters in a reproductive system with high male competition. The evaluated sperm quality parameters were: volume, density, viability, motility and sperm movement duration. High variability in sperm traits were found probably in response to the heterogeneity of individual condition, social status, spawning activity and migration costs. Duration of motility was positively correlated (P<0.05) with age class of breeders and motility. Stronger positive correlations (P<0.001) were observed between motility and viability and between sperm counts and viable sperms. This study reported the first data about semen quality of an Italian wild population of native trout. These results are useful for planning efficient artificial fertilization protocols in restoration programs involving supportive breeding together with other innovative conservation strategies as gamete cryopreservation.
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Akcay, E., Bozkurt, Y., Secer, S. and Tekin, N. 2004. Cryopreservation of mirror carp semen. Turkish Journal of Veterinary and Animal Sciences, 28(5): 837-843.
Allendorf, F. W. and Waples, R. S. 1996. Conservation and Genetics of Salmonid Fishes. In: J. C. Avise and J. J. Hamrick (Eds.), Conservation Genetics Case Stories from Nature, Chapman and Hall, New York: 238-280
Aral, F., Şahinöz, E. and Dogu, Z. 2007. A study on the milt quality of Oncorhynchus mykiss (Walbaum, 1972) and Carasobarbus luteus (Heckel, 1843) in Atatürk Dam Lake, Southeastern Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 7: 41-44.
Belmar-Lucero, S., Wood, J. L., Scott, S., Harbicht, A.B., Hutchings, J.A. and Fraser, D.J. 2012. Concurrent habitat and life history influences on effective/censuspopulation size ratios in stream-dwelling trout. Ecology and evolution, 2(3): 562-573. doi: 10.1002/ece3.196
Bianco, P. G., Caputo, C., Ferrito, V., Lorenzoni, M., Nonnis Marzano, F., Stefani, F., Sabatini, A. and Tancioni, L. 2013. Pesci d'acqua dolce. In: C. Rondinini, A. Battistoni, V. Peronace and L. Tancioni (Eds.), Lista Rossa IUCN dei Vertebrati Italiani. Comitato Italiano IUCN e Ministero dell'Ambiente edella Tutela del Territorio e del Mare, Roma: 1-54.
Bozkurt, Y., Seçer, S., Tekin, N. and Akçay, E. 2005. Cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus carpio) sperm with glucose based extender. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 1(1):21-25 (in Turkish).
Bozkurt, Y. 2006. The relationship between body condition, sperm quality parameters and fertilization success in rainbow trout (Oncorhynchus mykiss). Journal of Animal and Veterinary Advances, 5(4): 284-288. doi=javaa.2006.284.288
Bozkurt, Y., Seçer, S., Bukan, N., Akçay, E. and Tekin, N. 2006a. Relationship between body condition, physiological and biochemical parameters in brown trout (Salmo trutta fario) sperm. Pakistan Journal of Biological Sciences, 9(5): 940-944. doi: 10.3923/pjbs.2006.940.944
Bozkurt, Y., Secer, S. and Bejcan, S. 2006b. Relationship between spermatozoa motility, egg size, fecundity and fertilization success in Salmo trutta abanticus. Tarim bilimleri dergisi, 4: 345-348.
Bozkurt, Y., Öğretmen, F., Kökçü, Ö. and Ercin, U. 2011. Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility. Czech Journal of Animal Science, 56(8): 355-364.
Burnham, K. P., and Anderson, D. R. 2002. Model selection and multimodel inference: a practical informationtheoretic approach. Springer Science and Business Media.
Campton, D. E. 2004. Sperm competition in salmon hatcheries: the need to institutionalize genetically benign spawning protocols. Transactions of the American Fisheries Society, 133(5):1277-1289.
Canyurt, M. A. and Akhan, S. 2008. Effect of ascorbic acid supplementation on sperm quality of rainbow trout (Onchorynchus mykiss). Turkish Journal of Fisheries and Aquatic Sciences, 8: 171-175.
Casselman, S. J., Schulte-Hostedde, A. I. and Montgomerie. R. 2006. Sperm quality influences male fertilization success in Walleye (Sander vitreus). Canadian Journal of Fisheries and Aquatic Sciences, 63: 2119-2125. doi: 10.1139/F06-108
Champigneulle, A., Largiader, C. R. and Caudron, A., 2003. Reproduction de la truite (Salmo trutta L.) dans le torrent de Chevenne, Haute-Savoie. Un fonctionnement original? Bulletin Français de la P?che et de la Pisciculture 369: 41-70.
Ferguson, A. 2006. Genetics of sea trout with particular reference to Britain and Ireland. In: G.S. Harris and N.J. Milner (Eds.) Sea trout: biology, conservation and management, Blackwell publishing, Oxford: 157-182.
Fleming, I. A. and Gross, M. R. 1994. Breeding competition in a Pacific salmon (Coho: Oncorhynchus kisutch): measures of natural and sexual selection. Evolution, 48: 637-657. doi: 10.2307/2410475
Gage, M. J., Macfarlane, C. P., Yeates, S., Ward, R. G., Searle, J. B. and Parker, G. A. 2004. Spermatozoal traits and sperm competition in Atlantic salmon: relative sperm velocity is the primary determinant of fertilization success. Current biology, 14(1): 44-47. doi: 10.1016/j.cub.2003.12.028
Gausen, D. 1993. The Norwegian gene bank programme for Atlantic salmon (Salmo salar). In: J.C. Cloud and G.H. Thorgaad (Eds.), Genetic conservation of salmonid fishes, Plenum, New York, USA: 181-187.
Gibertoni, P., Jelli, F. and Bracchi P. 1998. Allevamento, riproduzione e reintroduzione in ambiente naturale di trote fario di ceppo mediterraneo, Salmo (trutta) trutta L. Annali della Facoltà di Medicina Veterinaria, Università di Parma, 18: 1-20.
Glogowski, J., Kwasnik, M., Piros, B., Dabrowski, K., Goryczko, K., Dobosz, S., Kuzminski H. and Ciereszko, A. 2000. Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquaculture Research, 31(3): 289-296. doi: 10.1046/j.1365-2109.2000.00400.x
Hajirezaee, S., Mojazi Amiri, B. and Mirvaghefi, A.R. 2009. Effect of stripping frequency on semen quality of endangered caspian Brown trout (Salmo trutta caspius). American Journal of Animal and Veterinery Science, 4(3): 65-71. doi:10.3844/ajavsp.2009.65.71
Hansen, M. M. and Loeschcke, V. 1994. Effects of releasing hatchery-reared brown trout to wild trout populations. In: V. Loeschcke, J. Tomiuk and S.K. Jain, (Eds.), Conservation Genetics, Birkhäuser Verlag, Basel: 273-289.
Hatef, A., Niksirat, H., Amiri, B.M., Alavi, S.M.H. and Karami, M. 2007. Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius). Aquaculture Research, 38:1175-1181. doi: 10.1111/j.1365-2109.2007.01784.x
Hatipoğlu, T. and Akçay, E. 2010. Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Üniversitesi Veteriner Fakültesi Dergisi, 57: 33-38.
Iaffaldano, N., Di Iorio, M., Manchisi, A., Esposito, S. and Gibertoni. P. P. (in press). Effective freezing rate for semen cryopreservation in endangered Mediterranean brown trout (Salmo trutta macrostigma) inhabiting Biferno River (South Italy). Zygote, 10.1017/S0967199415000647. 1-8.
IUCN. 1996. The 1996 IUCN Red List of threatened animals. (J. Baillie and B. Groombridge Eds.).
Janhunen, M., Rudolfsen, G., Kekäläinen, J., Figenschou, L., Peuhkuri, N. and Kortet, R. 2009. Spawning coloration and sperm quality in a large lake population of Arctic charr (Salmonidae: Salvelinus alpinus L.). Biological Journal of the Linnean Society, 98(4): 794- 802. doi: 10.1111/j.1095-8312.2009.01317.x
Jelli F. and Gibertoni P. 1999. Recupero e reintroduzione di ceppi autoctoni di trota fario, Salmo (trutta) trutta L., nel bacino del fiume Secchia. Atti del Convegno: Recupero e reintroduzione dei ceppi autoctoni di trota fario, Salmo (trutta) trutta L., di "ceppo mediterraneo" in ambienti appenninici tipici. Esperienze a confronto. 21-287. Reggio Emilia.
Johnson, K., Butts, I. A. E., Wilson, C. C. and Pitcher, T. E. 2013. Sperm quality of hatchery-reared lake trout throughout the spawning season. North American Journal of Aquaculture, 75: 102-108. doi: 10.1080/15222055.2012.711277
Laikre, L. 1999. Conservation Genetic Management of Brown Trout (Salmo trutta) in Europe. Division in Population Genetics, Stockholm University, Sweden: 5-50.
Leary, R. F., Allendorf, F. W. and Forbes, S. H. 1993. Conservation genetics and bull trout in the Columbia and Klamath River drainages. Conservation Biology, 7: 856-865. doi: 10.1046/j.1523-1739.1993.740856.x
Levitan, D. R. 2000. Sperm velocity and longevity trade off each other and influence fertilization in the sea urchin Lytechinus variegatus. Proceedings of the Royal Society of London B: Biological Sciences, 267(1443): 531-534.
Liley, N. R., Tamkee, P., Tsai, R. and Hoysak, D. J. 2002. Fertilization dynamics in rainbow trout (Oncorhynchus mykiss): effect of male age, social experience, and sperm concentration and motility on in vitro fertilization. Canadian Journal of Fisheries and Aquatic Sciences, 59(1): 144-152. doi: 10.1139/F01-202.
Lux, F. E., 1971. Age determination of fishes. US Department of Commerce, National Oceanic and Atmospheric Administration, National Marine Fisheries Service.
Martínez-Páramo, S., Pérez-Cerezales, S., Gómez-Romano, F., Blanco, G., Sánchez, J. A. and Herráez, M. P. 2009. Cryobanking as tool for conservation of biodiversity: Effect of brown trout sperm cryopreservation on the male genetic potential. Theriogenology, 71: 594-604. doi: 10.1016/j.theriogenology.2008.09.034
McNeil, W. J. 1964. Redd superimposition and egg capacity of pink salmon spawning beds. Journal of the Fisheries Board of Canada, 21(6): 1385-1396. doi: 10.1139/f64- 119
McNiven, M. A., Gallant, R. K. and Richardson, G. F. 1992. In vitro methods of assessing viability of rainbow trout spermatozoa. Theriogenology, 38: 679-686. doi: 10.1016/0093-691X(92)90030-U
Milner, N. J., Elliott, J. M., Armstrong, J. D., Gardiner, R., Welton, J. S. and Ladle, M. 2003. The natural control of salmon and trout populations in streams. Fisheries Research, 62(2): 111-125. doi:10.1016/S0165-7836(02)00157-1
Penserini, M., Nonnis Marzano, F., Gandolfi, G., Maldini, M., Marconato, E. and Gibertoni, P. P. 2006. Fenotipi della trota mediterranea: metodologia di indagine molecolare combinata e selezione morfologica per l'identificazione degli esemplari autoctoni. Journal of Freshwater Biology, 69-75.
Pitcher, T. E., Doucet, S. M., Beausoleil, J. M. and Hanley, D. 2009. Secondary sexual characters and sperm traits in coho salmon Oncorhynchus kisutch. Journal of Fish Biology, 74(7): 1450-1461. doi: 10.1111/j.1095-8649.2009.02210.x
Rainis, S., Gasco, L. and Ballestrazzi, R. 2005. Comparative study on milt quality features of different finfish species. Italian Journal of Animal Science, 4: 355-363. doi: 10.4081/ijas.2005.355
Rubin, J.-F., Glimsa¨ter, C. & Jarvi, T., 2004. Characteristics and rehabilitation of the spawning habitats of the sea trout, Salmo trutta, in Gotland (Sweden). Fisheries Management and Ecology 11: 15-22 DOI: 10.1111/j.1365-2400.2004.00349.x
Rudolfsen, G., Figenschou, L., Folstad, I., Tveiten, H. and Figenschou, M. 2006. Rapid adjustments of sperm characteristics in relation to social status. Proceedings of the Royal Society of London B: Biological Sciences, 273(1584): 325-332. doi: 10.1098/rspb.2005.3305
Rudolfsen, G., Figenschou, L., Folstad, I. and Kleven, O. 2008. Sperm velocity influence paternity in the Atlantic cod (Gadus morhua L.). Aquaculture Research, 39: 212-216. doi: 10.1111/j.1365- 2109.2007.01863.x
Rurangwa, E., Kime, D. E., Ollevier, F. and Nash, J. P. 2004. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234(1): 1-28. doi: 10.1016/j.aquaculture.2003.12.006
Ryman, N., Utter, F. and Laikre L. 1995a. Protection of intraspecific biodiversity of exploited fishes. Reviews in Fish Biology and Fisheries, 5: 417-446. doi: 10.1007/BF01103814
Ryman, N., Jorde, P.E. and Laikre, L. 1995b. Supportive breeding and variance effective population size. Conservation Biology, 9: 1619-1628. doi: 10.1046/j.1523-1739.1994.08030863-14.x
Şahin T., Kurto?lu I.Z., Balta F. 2013. Effect of different extenders and storage periods on motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) semen. Universal Journal of Agricultural Research, 1(3): 65-69. doi: 10.13189/ujar.2013.010304
Springate, J. R. C., Bromage, N. R. and Cumaranatuga, P. R. T. 1985. The effects of different rations on fecundity and egg quality in the rainbow trout (Salmo gairdneri). In: C.B. Cowey, M. Makie and J.G. Bell (Eds.), Nutrition and Feeding in Fish, Academic Press Lmtd, London, UK: 371-393.
Stockley, P., Gage, M. J. G., Parker, G. A. and Møller, A.P. 1997. Sperm competition in fishes: the evolution of testis size and ejaculate characteristics. The American Naturalist, 149(5): 933-954. doi: 10.1086/286031
Tuset, V. M., Dietrich, G. J., Wojtczak, M, S?owiñska, M., de Monserrat, J. and Ciereszko, A. 2008. Relationships between morphology, motility and fertilization capacity in Rainbow Trout (Oncorhynchus mykiss) spermatozoa. Journal of Applied Ichthyology, 24: 393- 397. doi: 10.1111/j.1439-0426.2008.01145.x
Wedekind, C., Rudolfsen, G., Jacob, A., Urbach, D. and Müller, R. 2007. The genetic consequences of hatchery-induced sperm competition in a salmonid. Biological Conservation, 137(2): 180-188. doi:10.1016/j.biocon.2007.01.025