Yaqiu LIN, Jishu ZHOU, Ruiwen Li, Xianrong XIONG, Pengfei WU

MRF Gene Family in Schizothorax Prenanti: Molecular Cloning, Tissue Expression, and mRNA Expression in Muscle Development

The myogenic regulatory factors (MRFs) play key roles in growth and muscle development in mammals and some fish, however previous studies about the cloning, tissue expression and mRNA expression in muscle development of MRFs genes in Schizothorax prenanti (S.prenanti) was scarce. In order to know how MRFs genes were expressed in different tissues and development period, we cloned the complete CDS of the MRF gene family from S.prenanti for the first time, including MyoD, MyoG, Myf5 and Myf6. Amino acid sequence alignment indicated that the S.prenanti MRF gene family has a basic helixloop-helix (HLH) domain. Quantitative real-time RT-PCR analysis indicated that MyoD, MyoG, Myf5 and Myf6 mRNA was highly expressed in red muscle and white muscle. The mRNA expression of MRF family in muscle during different developmental stages (larvae, juvenile and adult) in vivo were also investigated. The results showed that MyoG and Myf5 expression in muscle were significantly associated with muscle development. Our findings provided molecular characterization and expression profile of the MRF gene family in S.prenanti and enhanced understanding about the MRFs in muscle growth and development in fish.

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Bentzinger, C.F., Wang, Y.X. and Rudnicki, M.A. 2012. Building muscle: molecular regulation of myogenesis. CSH Perspect Biol, 4(2): a008342. doi: 10.1101/cshperspect.a008342;

Braun,T. and Gautel, M.2011.Transcriptional mechanisms regulating skeletal muscle differentiation, growth and homeostasis. Nat Rev Mol Cell Biol, 12: 349-361. doi:10.1038/nrm3118;

Bryson-Richardson, R.J. and Currie, P.D. 2008. The genetics of vertebrate myogenesis. Nat Rev Genet, 9: 632-646. doi: 10.1038/nrg2369;

Buckingham, M. and Vincent, S.D.2009. Distinct and dynamic myogenic populations in the vertebrate embryo. Curr Opin Genet Dev, 19: 444-453. doi: 10.1016/j.gde.2009.08.001;

Hinits, Y., Williams, V.C., Sweetman, D., Donn, T.M., Ma, T.P., Moens, C.B. and Hughes, S.M. 2011. Defective cranial skeletal development, larval lethalit y and haplo insufficiency in MyoD mutant zebrafish. Dev Biol, 358:102-112. doi:10.1016/j.ydbio.2011.07.015;

Holterman, C.E., Le Grand, F., Kuang, S., Seale, P. and Ruanicki, M.A. 2007. Megf10 regulates the progression of thesatellite cell myogenic program. J Cell Biol,179:911-922. doi: 10.1083/jcb.200709083;

Johnston, I.A. 1999. Muscle development and growth: potential implications for flesh quality in fish. Aquaculture, 177: 99-115.doi: 10.1016/S0044- 8486(99)00072-1;

Kablar, B., Asakura, A., Krastel, K., Ying, C. and May, L.L. 1998. MyoD and Myf-5 define the specification of musculature of distinct embryonic origin. Biochem Cell Biol,76: 1079-1091. doi: 10.1139/bcb-76-6-1079;

Kanisicak, O., Mendez, J.J., Yamamoto, S., Yamamoto, M. and Goldhamer, D.J. 2009. Progenitors of skeletal musclesatellite cells express the muscle determination gene,MyoD. Dev Biol, 332:131-141. doi: 10.1016/j.ydbio.2009.05.554;

Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H. and Tsai, H.J.2006.Myogenic regulatory factors Myf5 and MyoD function distinctly during craniofacial myogenesis of zebrafish. Dev Biol Nov, 299:594-608. doi:10.1016/j.ydbio.2006.08.042;

Liu, H., Wang, J., Si, J., Jia, J., Li, L., Han, C., Huang, K., He, H. and Xu, F. 2012. Molecular cloning and in silico analysis of the duck (Anas platyrhynchos) MEF2A gene cDNA and its expression profile in muscle tissues during fetal development. Genet Mol Biol, 35:182-190. doi: 10.1590/S1415- 47572012005000023;

Macqueen, D.J., Robb, D. and Ian, A. 2007. Johnston. Temperature influences the coordinated expression of myogenic regulatory factors during embryonic myogenesis in Atlantic salmon (Salmo salar L.). J Exp Biol, 210: 2781-2794. doi:10.1242/jeb.006981;

Megeney, L.A. and Rudnicki, M.A..1995. Determinationversus differentiation and the MyoDfamily of transcriptionfactors. Biochem. Cell Biol, 73: 723-732. doi:10.1101/sqb.2008.73.064;

Parker, M.H, Seale, P. and Rudnicki, M.A. 2003. Looking back to the embryo: defining transcriptional networks in adult myogenesis. Nat Rev Genet, 4: 497-507. doi:10.1038/nrg1109;

Schnapp, E., Pistocchi, A.S., Karampertsou, E., Foglia, E., Lamia, C.L., Cotelli, F. and Cossu, G.. 2009. Induced earlyexpression of mrf4 but not myog rescues myogenesis inthe MyoD/myf5 double-morphant zebrafish embryo.J Cell Sci, 122:481-488. doi: 10.1242/jcs.038356;

Song, J., Song, Z., Yue, B. and Zheng, W.2006.Assessing genetic diversity of wild populations of Prenantä9s schizothoracin, Schizothorax prenanti, using AFLP markers. Environ Biol Fish, 77: 79-86. doi: 10.1007/s10641-006-9056-x;

Te Pas, M.F., Soumillion, A., Harders, F.L., Verburg F.J. and van den Bosch, T.J., Galesloot, P.and Meuwissen, T.H. 1999. Influences of myogenin genotype on birth weight,growth rate, carcass weight, backfat thickness and lean weight of pigs. J Anim Sci, 77: 2352-2356. doi:/1999.7792352x;

Weintraub, H., Davis,R., Tapscott, S., Thayer, M., Krause, M., Benezra, R., Blackwell, T.K., Turner, D., Rupp, R. and Hollenberg, S. 1991. The myoD gene family: Nodal point during specification of the muscle cell lineage. Science, 251:761-766. doi: 10.1126/science.1846704;

Wyszyn´ ska-Koko, J., Pierzchala, M., Flisikowski, K., Kamyczek, M., Ro´ zycki M. and Kuryl,J.P.2006. Polymorphisms incoding and regulatory regions of the porcine MYF6 and MYOG genes and expression of the MYF6 gene in m.longissimus dorsi versus productive traits in pigs. J Appl Genet, 47: 131-138. . doi: 10.1007/BF03194612;

Yafe, A., Shklover, J., Weisman-Shomer, P., Bengal, E. and Fry, M. 2008. Differential binding of quadruplex structures ofmuscle-specific genes regulatory sequences by MyoD, MRF4 and myogenin. Nucleic Acids Res, 36: 3916-3925. doi: 10.1093/nar/gkn340;

Yokoyama,S. and Asahara, H.2011. The myogenic transcriptional network. Cell Mol Life Sci, 68:1843- 1849. doi: 10.1007/s00018-011-0629-2;

Zou, G.W., Zhu, Y.Y., Liang, H.W. and Zhong, L.2015.Association of pituitary adenylate cyclaseactivating polypeptide and myogenic factor 6 genes with growth traits in Nile tilapia (Oreochromis niloticus). Aquacult Int. doi: 10.1007/s10499-015- 9878-7;

Zhu, X., Li. Y.L., Liu, L., Wang, J.H., Li, H.H., Wu,P., Chu, W.Y. and Zhang, J.S.2016. Molecular characterization of Myf5 and comparative expression patterns of myogenic regulatory factors in Siniperca chuatsi. Gene Expr Patterns. 20(1):1-10. doi: 10.1016/j.gep.2015.10.003;