Effect of Different Photoperiod Regimes on Sperm Quality, Fecundity and Fertilization in Rainbow Trout (Oncorhynchus mykiss)

Sunulan bu çalışma, farklı fotoperyot rejimlerinin gökkuşağı alabalığı (Oncorhynchus mykiss) damızlıklarının sperma kalite parametreleri, ovulasyon/spermiasyon süreleri ve kuluçka performansları üzerine olan etkilerini belirlemek amacıyla yürütülmüştür. Bu çalışma, farklı uzun ve kısa fotoperyot rejimlerinin kombinasyonu (Grup I: 18A:6K ve 18K:6A; Grup II: 14A:10K ve 14K:10A ve doğal aydınlanma (kontrol grubu)) şeklinde planlanmıştır. Bütün uygulamalar her grupta üç tekerrürlü olacak şekilde yürütülmüştür. Çalışma sonuçlarına göre, en yüksek ortalama spermatozoa motilitesi (%83,0±2,1) ve motilite süresi (67,2±6,3 s) kontrol grubunda belirlenirken, dişi gökkuşağı alabalığı damızlıklarında en uzun ovulasyon I. grupta 265 günde gerçekleşmiştir. Her ne kadar en yüksek ortalama mutlak yumurta verimi 3654,7±298,3 yumurta/balık olarak I. grupta belirlense de, en yüksek ortalama relatif yumurta verimi 137,3±24,5 yumurta/kg olarak kontrol grubunda belirlenmiştir. Ayrıca, en büyük ortalama yumurta çapı (4,6±0,1 mm) ve en yüksek fertilizasyon oranı (%87,0±2,5) kontrol grubunda belirlenmiştir. İstatiksel analizler, tüm fotoperyot rejimlerinde; spermatozoa motilitesi, spermatozoa motilite süresi ve spermatozoa yoğunluğu ile fertilizasyon oranları arasında pozitif korelasyon olduğunu göstermiştir (P>0,05). Diğer taraftan, tüm fotoperyot rejimlerinde sperma hacmi ile sperma pH'sı, fertilizasyon oranları ile negatif korelasyon göstermiştir (P>0,05). İstatiksel olarak önemli tek pozitif korelasyon, relatif fekundite ile fertilizasyon arasında 18A:6K/18K:6A fotoperyot rejiminde belirlenmiştir (r=0,452; P

Farklı Fotoperiyot Rejimlerinin Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Sperma Kalitesi, Fekundite ve Fertilizasyon Üzerine Etkisi

The present study was carried out to determine effect of different photoperiod regimes on sperm quality parameters, ovulation/spermiation time and hatchery performance of rainbow trout (Oncorhynchus mykiss) broodstock. The designation was done as combination of different long and short photoperiod regimes such as: 18L:6D and 18D:6L (group I); 14L:10D and 14D:10L (group II) and natural lighting (control group). All treatments were carried out as three replications at each group. As a result, the highest mean spermatozoa motility (83.0±2.1 %) and motility period (67.2±6.3 s) were determined in control group. It was determined that the longest ovulation was occured in female rainbow trout broodstock at 265 days in group I. Although the highest mean absolute egg productivity was determined as 3654.7±298.3 eggs/fish in group I, the highest mean relative egg productivity was determined as 137.3±24.5 eggs/kg in control group. Furthermore, the highest mean egg diameter (4.6±0.1 mm) and fertilization rate (87.0±2.5 %) were determined in control group. Statistical analyses revealed that spermatozoa motility, spermatozoa motility period and spermatozoa density positively correlated with fertilization rate in all photoperiod regimes (P>0.05). On the other hand, semen volume and semen pH negatively correlated with fertilization rate in all photoperiod regimes (P>0.05). It is interesting to note that only statistically important positive correlation was determined between relative fecundity and fertilization rate in 18L:6D/18D:6L photoperiod regime (r=0.452, P<0.05). Consequently, results revealed that combined long and short artificial photoperiod regimes can advance ovulation and spermiation and also can effect gamete quality and hatchery performance of rainbow trout during out-of-season spawning

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