LEVENT BAT, Hasan Can ÖZTEKİN, FUNDA ÜSTÜN

Heavy Metal Levels in Four Commercial Fishes Caught in Sinop Coasts of the Black Sea, Turkey

Bu çalışmanın amacı 2012-2013 balıkçılık sezonunda Karadeniz'in Sinop kıyılarından yakalanan barbun (Mullus barbatus), mezgit (Merlangius merlangus), istavrit (Trachurus mediterraneus) ve altınbaş kefal (Liza aurata) balıklarının kas dokularında sekiz ağır metalin (alüminyum, arsenik, bakır, çinko, cıva, demir, kadmiyum ve kurşun) düzeylerini değerlendirmektir. Balıklardaki metal analizleri ICP-MS (endüktif eşleşmiş plazma - kütle spektrometresi) kullanılarak gerçekleştirilmiştir. T. mediterraneus türündeki Cu miktarı hariç tüm balık türlerindeki Al, Hg, Cd, Pb ve Cu düzeyleri ölçüm düzeylerinin altında bulunmuştur. İstavritteki ortalama Cu miktarı 0.67 mg/kg yaş ağırlıktır. En yüksek Zn (24,7 mg/kg yaş ağırlık) yine T. mediterraneus türünde bulunmuş olup diğer türlerdeki değerlerden 7-8,5 kez daha fazladır. Diğer türler ile karşılaştırıldığında L. aurata türü en yüksek Fe (3,2 mg/kg yaş ağırlık) ancak en düşük As (0,25 mg/kg yaş ağırlık) değerine sahiptir. M. barbatus türündeki As düzeyleri yüksek (1,3 mg/kg yaş ağırlık) olup bunu M. merlangustürü (1,24 mg/kg yaş ağırlık) izlemiştir. Çalışılan ağır metal konsantrasyonları Türk Gıda Kodeksi ve Avrupa Birliği Tüzüğü'nce belirlenen değerlerin oldukça altında bulunmuştur. Bu nedenle, bu ağır metallerin toplam vücut yükündeki payının önemsenmeyecek kadar küçük olarak nitelendirilebilir

Karadeniz'in Sinop Kıyılarında (Türkiye) Yakalanan Dört Ticari Balıkta Ağır Metal Düzeyleri

The aim of this study is to evaluate the levels of eight heavy metals (aluminium, arsenic, copper, zinc, mercury, iron, cadmium and lead) in the muscle tissue of Red mullet (Mullus barbatus), Whiting (Merlangius merlangus), Mediterranean horse mackerel (Trachurus mediterraneus) and Golden grey mullet (Liza aurata) caught from Sinop coast of the Black Sea during fishing season in 2012 and 2013. Metal analyses in fish were performed using ICP-MS (Inductively Coupled Plasma - Mass Spectrometer).The levels of Al, Hg, Cd, Pb and Cu in all fish species except T. mediterraneus for Cu were below the limit of detections. Mean Cu level in Mediterranean horse mackerel was 0.67 mg/kg wet wt. Highest value of Zn (24.7 mg/kg wet wt) was also found in the T. mediterraneus which were about 7-8.5 times more than the other species.L. aurata have the highest Fe value (3.2 mg/kg wet wt), but have the lowest As value (0.25 mg/kg wet wt.) compared with the other fish species. The levels of As for M. barbatus were high (1.3 mg/kg wet wt.) and followed by M. merlangus (1.24 mg/kg wet wt).The concentrations of the studied heavy metals were far below the established values by the Turkish Food Codex and Commission Regulation (EC). Therefore, their contribution to the total body burden of these heavy metals can be considered as negligibly small

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Altas, L. and Büyükgüngör, H. 2007. Heavy metal pollution in the Black Sea shore and offshore of Turkey. Environmental Geology, 52: 469-476. doi: 10.1007/s00254-006-0480-1.Bahnasawy, M., Khidr, A.Z. and Dheeina, N. 2011. Assessment ofheavy metal concentrations in water, planktonandfish of Lake Manzala, Egypt. Turk.J.Zool., 35(2): 271-280.doi:10.3906/zoo-0810-6.Bakan, G., Özkoç,H.B., Büyükgüngör, H., Ergun, O.N. and Onar,N. 1996. Evaluation of the Black Sea and-based sources inventory results of the coastal region of Turkey. Proc. of the International Workshop on MED and Black Sea ICZM; 1996 November 2-5; pp: 39-52.Bakan, G.andBüyükgüngör, H. 2000. The Black Sea. Marine Pollution Bulletin, 41: 24-43. doi: 10.1016/S0025-326X(00)00100-4.Bat, L., Öztürk, M. and Öztürk, M. 1996. Heavy metal amounts in some commercial teleost fish from the Black Sea. O.M.Ü. Fen Dergisi, 7: 117-135.Bat, L., Gündoğdu, A., Yardım, Ö., Zoral, T. and Çulha, S. 2006. Heavy metal amounts in zooplanktonand some commercial teleost fish from inner harbour of Sinop, Black Sea. SÜMDER (Su Ürünleri Müh. Dergisi), 25: 22-27.Bat, L., Gökkurt, O., Sezgin, M., Üstün, F. and Sahin, F. 2009. Evaluation of the Black Sea land based sources of pollution the coastal region of Turkey. The Open Marine Biology Journal, 3: 112-124.Bat, L., Sezgin, M., Üstün, F. and Şahin, F. 2012. Heavy metal concentrations in ten species of fishes caught in Sinop coastal waters of the Black Sea, Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 12: 371-376.doi: 10.4194/1303-2712-v12_2_24Bat, L., Sezgin., M., Gökkurt Baki, O., Üstün, F.andŞahin, F. 2013. Determination of heavy metals in some commercial fish from the Black Sea coast of Turkey. Walailak J.Sci.and Tech., 10: 581-589.Bat, L. 2014. Heavy metal pollution in the Black Sea. In: E.Düzgüneş, B.Öztürk, andM.Zengin (Eds.),Turkish Fisheries in the Black Sea. Published by Turkish Marine Research Foundation (TUDAV), Publication number: 40, Istanbul:71-107.Boran, M. and Altinok, I. 2010. A review of heavy metals in water, sediment and living organisms in the Black Sea. Turkish Journal of Fisheries and Aquatic Sciences, 10(4): 565-572.doi: 10.4194/trjfas.2010.0418Bryan, G.W. 1976. Heavy Metal Contamination in the Sea. In: R. Johnston (Ed.), Marine Pollution, Academic Press Inc.,London: 185-302.Bustamante, P., Bocher, P., Chérel, Y., Miramand, P. and Caurant, A. 2003. Distribution of trace elements in the tissues of benthic and pelagic fish from the Kerguelen Islands. The Science of the Total Environment 313: 25-39.doi:10.1016/S0048-9697(03)00265-1Council of Europe 2001. Council of Europe's policy statements concerning materials and articles intended to come into contact with foodstuffs. Policy Statement concerning materials and alloys. Technical Document. Guidelines on metals and alloys used as food contact materials (09.03.2001), Strasbourg,67 pp.Dalman, Ö., Demirak, A. and Balcı, A. 2006. Determination of heavy metals (Cd, Pb) and trace element (Cu, Zn) in sediment and fish of the Southeastern Aegean Sea (Turkey) by Atomic Absorption Spectrometry. Food Chemistry, 95: 157-62.doi: 10.1016/j.foodchem.2005.02.009Das, Y.K., Aksoy, A., Baskaya, R., Duyar, H.A., Güvenc, D. and Boz, V. 2009. Heavy metal levels of some marine organisms collectes in Samsun and Sinop Coasts of Black Sea, in Turkey. Journal of Animal and Veterinary Advances, 8(3): 496-499.Directorate-GeneralHealth and Consumer Protection 2004. Reports on tasks for scientific cooperation. Assessment of the dietary exposure to arsenic, cadmium, lead and mercury of the population of the EU Member States. Report of experts participating in Task 3.2.11. 125 pp. http://ec.europa.eu/food/food/chemicalsafety/contaminants/scoop_3-2-11_heavy_ metals_report_en.pdf. Accessed on 06.06.2014.FAO/WHO 2010. Summary report of the seventy-third meeting of JECFA. Joint FAO/WHO Expert Committee on Food Additives. Geneva.Kojadinovic, J., Potier, M., Le Corre, M., Cosson, R.P. and Bustamante,P. 2007. Bioaccumulation of trace elements in pelagic fish from the Western Indian Ocean Environmental Pollution, 146(2): 548-566. doi: 10.1016/j.envpol.2006.07.015.MAFF(The Ministry of Agriculture, Forestry and Fisheries) 1995. Monitoring and surveillance of non-radioactive contaminants in the aquatic environment and activities regulating the disposal wastes at sea. Directorate of Fisheries research, Lowestoft, Aquatic Environment Monitoring Report, No 44.Mendil, D., Demirci, Z., Tüzen, M. and Soylak, M. 2010. Seasonal investigation of trace element contents in commercially valuable fish species From the Black Sea, Turkey. Food and Chemical Toxicology, 48: 865-870.doi:10.1016/j.fct.2009.12.023Nisbet, C., Terzi, G., Pilger, O.andSarac, N. 2010. Determination of heavy metal levels in fish sample collected from the Middle Black Sea. Kafkas Üniv.Vet. Fak. Der., 16: 119-125.Official Journal of the European Communities 2000. Directive 2000/60/EC of the European Parliament and of the Councilof 23 October 2000 establishing a framework for Community action in the field of water policy,327:1-72.Official Journal of the European Union2008. Directives Directive 2008/56/EC ofthe European Parliament and of the Council of 17 June 2008 establishing a framework for community action in the field of marine environmental policy (Marine Strategy Framework Directive). L 164: 19-40.Özsoy, E. and Ünlüata, Ü.1997. Oceanography of the Black Sea: A review of some recent results. Earth Sci. Rev.,42(4): 231-272.Phillips, D.J.H. 1977. The use of biological indicator organisms to monitor trace metal pollution in marine and estuarine environments. A review. Environ. Pollut., 13: 281-317.Taghavi Jelodar, H., Sharifzadeh Baei, M., Najafpour, Sh. and Fazli, H. 2011. The comparison of heavy metals concentrations in different organs of Liza auratainhabiting in Southern Part of Caspian Sea. World Applied Sciences Journal (Special Issue of Food and Environment), 14: 96-100.Tepe, Y., Türkmen, M. and Türkmen, A. 2007. Assessment of heavy metals in two commercial fish species of four Turkish seas. Environmental Monitoring and Assessment.doi: 10.1007/s10661-007-0079-3.TGK2002. Official Gazette of Republic of Turkey. Notifications about determination of the maximum levels for certain contaminants in foodstuffs of Turkish Food Codex (in Turkish). (Notification No: 2002/63), Issue: 24885.The Earth Report 3. 1992. An A-Z Guide to environmental issues. (General Eds.) Goldsmith, E. and Hildyard, N.) London, 175 pp.Topçuoğlu, S., Erentürk, N., Saygı, N., Kut, D., Esen, N., Başsarı, A. and Seddigh, E. 1990. Trace metal levels of fish from the Marmara and Black Sea. Toxicological and Environ.Chemistry,29: 95-99.Topçuoğlu, S., Kirbasoglu, Ç.andGüngör, N. 2002. Heavy metals in organisms and sediments from Turkish coast of the Black Sea, 1997-1998. Environment International, 27: 521-526. PII: S0160-4120(01)00099-X.Topping, G. 1973. Heavy metals in fish from Scottish waters. Aquaculture,11: 373-377.TUIK, Turkish Fishery Statistics.2014. http://www.tuik.gov.tr/. Accessed 9.12.2014.Turan, C., Dural, M., Oksuz, A. and Öztürk, B. 2009. Levels of heavy metals in some commercial fish species captured from the Black Sea and Mediterranean coast of Turkey. Bull Environ. Contam. Toxicol.,82:601-604.doi:10.1007/s00128-008-9624-1Türk Çulha, S., Bat, L., Çulha, M., Efendioglu, A.andaç, M. and Bati, B. 2007. Heavy metals levels in some fishes and molluscs from Sinop Peninsula of the Southern Black Sea, Turkey. Rapp. Comm. Int. Mer Medit.,38:323.Türkmen, M., Türkmen, A. and Tepe, Y. 2008. Metal contaminations in five fish species from Black, Marmara, Aegean and Mediterranean Seas, Turkey. J. Chil. Chem. Soc., 53(1): 1435-1439.Doi:10.4067/S0717-97072008000100021.Türkmen, M., Türkmen, A., Tepe, Y., Töre, Y. and Ateş, A. 2009. Determination of metals in fish species from Aegean and Mediterranean seas. Food Chemistry, 113: 233-237. doi:10.1016/j.foodchem.2008.06.071.Tüzen, M. 2009. Toxic and essential trace elemental contents in fish species from the Black Sea, Turkey. Food and Chemical Toxicology, 47:1785-1790.doi:10.1016/j.fct.2009.04.029Uluozlu, O.D., Tüzen, M., Mendil, D. and Soylak, M. 2007. Trace metal content in nine species of fish from the Black and Aegean Seas, Turkey. Food Chemistry, 104(2): 835-840.doi:10.1016/j.foodchem.2007.01.03Ünsal, M., Bekiroğlu, Y., Akdoğan, Ş, Ataç, Ü., Kayıkçı, Y., Alemdağ, N., Aktaş, M.andYıldırım C. 1993. Determination of heavy metals in some economically important marine organisms in southwestern Black Sea. TUBITAK Project No: DEBAG-80/G, Ankara,78 pp.(in Turkish).WHO1996. Trace elements in human nutrition and health. ISBN 92 4 156173 4 (NLM Classification: QU 130). Geneva.Zar, J.H. 1984. Biostatistical analysis. Prentice Hall, Int., New Jersey, 718pp.