M. HEIDARIEH, M. SOLTANI, A. H. TAMIMI, M. H. TOLUEI

Comparative effect of raw fiber (vitacel) and alginic acid (ergosan) on growth performance, immunocompetent cell population and plasma lysozyme content of giant sturgeon (huso huso)

Yetişkin mersin balıklarının (Huso huso) 90 gün boyunca yemine %0,5 oranında Ergosan ve %1,3 oranında Vitacel’in katılarak beslenmesi sonrası lenfosit, nötrofil ve eozinofil populasyonlarını da içeren hücre sayıları; bağışıklık yapısı, plazmadaki lizozom aktivitesi ve büyüme performansı değerlendirilmiştir. Besleme denemesi boyunca 15. ve 90. günde örnekleme yapılmıştır. Genel olarak, 90 günlük yemleme sonrası Ergosan alan balıklardaki lenfosit sayısı hem Vitacel alan balıklara hem de kontrol grubuna kıyasla önemli derecede (P

Anahtar Kelimeler:

lizozim, Huso huso, diyetler, enzim aktivitesi, eozinofil, lif, büyüme oranı, İmmün kompetans, canlı ağırlık artışı, lenfositler, alginik asit, kan plazması, nötrofil, ağızdan ilaç verilişi, mersin balığı

Gelişkin mersin balıklarının (huso huso) büyüme performansına, hücre popülasyonunun bağışıklık yetisine ve plazma lizozom içeriğine ham selüloz (vitacel) ve alginik asit (ergosan)’ın karşılaştırmalı etkileri

The imunnocompetent cell population size including lymphocyte, neutrophil and eosinophil populations, lysozyme activity in plasma and the growth performance were assessed in great sturgeon Huso huso after oral administration of Ergosan at 0.5% of the feed and Vitacel at 1.3% of the feed for 90 days. Sampling was performed on 15 and 90 days during feeding trial. Generally, a significant increase was seen in the lymphocyte count of fish received Ergosan compared to both Vitacel and control groups 90 days post-treatment (P<0.05). Interstingly, at the Neutrophil profile of fish fed Vitacel and Vitacel- Ergosan combination was observed significant increase on 90 days after trial (P<0.05). The number of eosinophils tended to be higher in Vitacel group (P<0.05) on day 90 and was significantly higher (P<0.05) than in Ergosan and control. Also, lysozyme activity in plasma of treatment fish by Ergosan and Vitacel was significantly higher than control group on 15 and 90 days post-treatment (P<0.05). In addition, the weight of fish fed diet containing Ergosan and/or Vitacel had a significant increase compared to control group on days 15 and 90 of trial (P<0.05).

Keywords:

lysozyme, Huso huso, diets, enzyme activity, eosinophils, fibre, growth rate, immune competence, liveweight gain, lymphocytes, alginic acid, blood plasma, neutrophils, oral administration, sturgeons,

PDF

___

Backers, T. and Syha, H. 2007. Raw fibre concentrates: revolution in feeding: A new European raw fibre concentrate improves feed conversion and reduces losses. J. Canadian Poul., 30-33.
Cohn, Z.A. and Wiener, E. 1963. The particulate hydrolases of macrophages. J. Exp. Med., 118: 1009-1023.
Cross, M., Mangelsdorf, I., Wedel, A. and Renkawitz, R. 1988. Mouse lysozyme M gene: isolation, characterization, and expression studies. Proceedings of the National Academy of Science of United States of America, 85: 6232–6236.
Dalmo, R.A. and Seljelid, R. 1995. The immunomodulatory effect of LPS, laminarian on Atlantic salmon, Salmo salar L., macrophages in vitro. J. Fish Dis., 18: 175-185.
Dohrman, A., Tsuda, T., Escudier, E., Cardone, M., Jany, B., Gum, J., Kim, Y. and Basbaum, C. 1994. Distribution of lysozyme and mucin (MUC2 and MUC3) mRNA in human bronchus. Exp. Lung. Res., 20: 367–380.
Ellis, A.E. 1990. Lysozyme assays. Tech. Fish Immunol., 101-103.
Engstad, R.E., Robertsen, B. and Frivold, E. 1992. Yeast glucan induces increase in lysozyme and complementmediated haemolytic activity in Atlantic salmon blood. Fish Shellfish Immunol., 2: 287-297.
Faghani, T., Kousha, A., Azari Takami, G.H. and Faghani, S. 2008. Study on Growth Performance, Survival Rate, Hematological Parameters in Rainbow Trout (Oncorhynchus mykiss) in Mazandaran Province of Iran. J. Fish. Aquatic Sci., 3: 398-403.
Fletcher, T.C. and White, A. 1973. Lysozyme activity in plaice (Pleuronectes platessa L.). Experientia, 29: 1283–1285.
Heidarieh, M., Afsharnasab, M., Soltani, M., Dashtyannasab, A., Rajabifar, S., Sheikhzadeh, N. and Tamimi, A.H. 2010. Effects of ergosan and vibromax to prevent vibriosis and WSSV in Litopeaneus vannamei. J. Fish. Aquat. Sci., 5: 120-125.
Jang, S.I., Hardie, L.J. and Secombes, C.J. 1995. Elevation of rainbow trout Oncorhynchus mykiss macrophage respiratory burst activity with macrophage-derived supernatants. J. Leukocyte Biol., 57: 943–947.
Johnston, L.J., Noll, S., Renteria, A. and Shurson, J. 2003. Feeding By-Products High in Concentration of Fiber to Nonruminants. Presented at the 3rd National Symposium on Alternative Feeds for Livestock and Poultry, Conference Proceedings, November 4-5 Kansas City: 169-186.
Kaufhold, J., Hammon, H.M. and Blum, J.W. 2000. Fructo-Oligosaccharide Supplementation: Effects on Metabolic, Endocrine and Hematological Traits in Veal Calves. J. Vet. Med. A., 47: 17–29.
Khoshbavar-Rostami, H.A., Soltani, M. and Hassan,H.M.D. 2006. Immune response of great sturgeon (Huso huso) subjected to long-term exposure to sublethal concentration of the organophoapliate, diazinon. J. Aqua., 256: 88-94.
Montero-Rocha, A., McIntosh, D., Sánchez-Merino, R. and Flores, I. 2006. Immuno stimulation of white shrimp (Litopenaeus vannamei) following dietary administration of Ergosan. J. Invert. Pathol., 91: 188-194.
Oh, C.H. and Choi, S.H. 1998. Effects of sodium alginate on the non-specific defence system of the common carp (Cyprinus carpio L.). Korean J. Immunol., 21: 71-76.
Paulsen, S.M., Lunde, H., Engstad, R.E. and Robertsen, B. 2003. In vivo effects of β-glucan and LPS on regulation of lysozyme activity and mRNA expression in Atlantic salmon (Salmo salar L.). Fish Shellfish Immunol., 14: 39-54.
Peddie, S., Zou, J. and Secombes, C.J. 2002. Immuno stimulation in the rainbow trout (Oncorhynchus mykiss) following intraperitoneal administration of Ergosan. J. Vete. Immunol, and Immunopathol., 86: 101-113.
Pitol, D.L., Mardegan Issa, J.P. and Caetano, H. 2007. Morphological Characterization of the Leukocytes in Circulating Blood of the Turtle (Phrynops hilarii). Int. J. Morphol., 25(4): 677-682.
Robertsen, B., Engstad, R. and Jørgensen, J.B. 1994. β-Glucans as immunostimulants in fish. In: J.S. Stolen and T.C. Fletcher, (Eds.), Modulators of Fish Immune Response. SOS Publications. Fair Haven, NewJersey: 83-99.
Robertsen, B., Roerstad, G., Engstad, R.E. and Raa, J. 1990. Enhancement of non-specific disease resistance in Atlantic salmon, Salmo salar L., by glucan from Saccharomyces cervisiae cell walls. Fish Dis., 13: 391-400.
Saurabh, S. and Sahoo, P.K. 2008. Lysozyme: an important defense molecule of fish innate immune system. J. Aqua. Res., 39: 223-239.
Shadkhast, M., Shabazki, H.R., Bigham-Sadegh, A., Shariati, S.E., Mahmoudi, T. and Shariffian-Fard, M. 2010. The Morphological characterization of the blood cells in the central Asian tortoise (Testudo horsfieldii). Vet. Res. Forum, 3: 134-141.
Warr, G.W. and Simon, R.C. 1983. The mitogen response potential of lymphocytes from the rainbow trout (Salmo gairdneri) re-examined. Dev. Comp. Immunol., 7: 379-384.
Yousif, A.N., Albright, L.J. and Evelyn, T.P.T. 1994. In vitro evidence for the antibacterial role of lysozyme in salmonid eggs. Dis. Aquat. Org., 19: 15-19.