Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image

The need to easily and quickly count larger numbers of nanoparticles, in order to obtain statistically useful size and size-distribution data, is addressed via the use of a readily available, free, public-domain program for particle counting, NIH-Image (and 2 others derived from it, Scion Image and Image J), collectively referred to herein as Image. The best protocols that we have found useful for the use of Image are reported; both appropriate as well as problematic applications of Image are then illustrated with a series of TEM images of Ir(0), Pd(0) and Au(0) nanoclusters. Methods to detect and image nanoclusters with sub-1-nm core diameters are reported and illustrated in the literature with Au nanoclusters, an important problem since the literature indicates that subnanometer Au nanoclusters are often present, but undetected. A list of suggestions and caveats for the appropriate use of Image is also provided, since this contribution is directed at first-time users who are not presently using nanoparticle imaging software. The reader is also reminded of several, well-known caveats for the use of TEM in obtaining size data for nanostructures. Overall, Image is a free, public-domain program that is useful for the rapid counting of large numbers of particles.

Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image

The need to easily and quickly count larger numbers of nanoparticles, in order to obtain statistically useful size and size-distribution data, is addressed via the use of a readily available, free, public-domain program for particle counting, NIH-Image (and 2 others derived from it, Scion Image and Image J), collectively referred to herein as Image. The best protocols that we have found useful for the use of Image are reported; both appropriate as well as problematic applications of Image are then illustrated with a series of TEM images of Ir(0), Pd(0) and Au(0) nanoclusters. Methods to detect and image nanoclusters with sub-1-nm core diameters are reported and illustrated in the literature with Au nanoclusters, an important problem since the literature indicates that subnanometer Au nanoclusters are often present, but undetected. A list of suggestions and caveats for the appropriate use of Image is also provided, since this contribution is directed at first-time users who are not presently using nanoparticle imaging software. The reader is also reminded of several, well-known caveats for the use of TEM in obtaining size data for nanostructures. Overall, Image is a free, public-domain program that is useful for the rapid counting of large numbers of particles.

Kaynak Göster

Bibtex @ { tbtkchem142572, journal = {Turkish Journal of Chemistry}, issn = {1300-0527}, eissn = {1303-6130}, address = {}, publisher = {TÜBİTAK}, year = {2006}, volume = {30}, pages = {1 - 13}, doi = {}, title = {Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image}, key = {cite}, author = {Woehrle, Gerd H. and Hutchıson, James E. and Özkar, Saim and Fınke, Richard G.} }
APA Woehrle, G , Hutchıson, J , Özkar, S , Fınke, R . (2006). Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image . Turkish Journal of Chemistry , 30 (1) , 1-13 .
MLA Woehrle, G , Hutchıson, J , Özkar, S , Fınke, R . "Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image" . Turkish Journal of Chemistry 30 (2006 ): 1-13 <
Chicago Woehrle, G , Hutchıson, J , Özkar, S , Fınke, R . "Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image". Turkish Journal of Chemistry 30 (2006 ): 1-13
RIS TY - JOUR T1 - Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image AU - Gerd H. Woehrle , James E. Hutchıson , Saim Özkar , Richard G. Fınke Y1 - 2006 PY - 2006 N1 - DO - T2 - Turkish Journal of Chemistry JF - Journal JO - JOR SP - 1 EP - 13 VL - 30 IS - 1 SN - 1300-0527-1303-6130 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Chemistry Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image %A Gerd H. Woehrle , James E. Hutchıson , Saim Özkar , Richard G. Fınke %T Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image %D 2006 %J Turkish Journal of Chemistry %P 1300-0527-1303-6130 %V 30 %N 1 %R %U
ISNAD Woehrle, Gerd H. , Hutchıson, James E. , Özkar, Saim , Fınke, Richard G. . "Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image". Turkish Journal of Chemistry 30 / 1 (Ocak 2006): 1-13 .
AMA Woehrle G , Hutchıson J , Özkar S , Fınke R . Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image. Turk J Chem. 2006; 30(1): 1-13.
Vancouver Woehrle G , Hutchıson J , Özkar S , Fınke R . Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image. Turkish Journal of Chemistry. 2006; 30(1): 1-13.
IEEE G. Woehrle , J. Hutchıson , S. Özkar ve R. Fınke , "Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image", Turkish Journal of Chemistry, c. 30, sayı. 1, ss. 1-13, Oca. 2006
  • ISSN: 1300-0527
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

12b 6.8b

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