Abdul Nasir KHALID, Habib AHMAD, Najam-ul-Sahar AFSHAN, Hassan SHER, Shah HUSSAIN

Xanthagaricus pakistanicus sp. nov. (Agaricaceae): fi rst report of the genus from Pakistan

Xanthagaricus pakistanicusis described as a new species from lowland northern Pakistan, based on morphological andmolecular data. It is characterized by a yellowish pileus, covered with dark brown squamules, a stipe with yellowish fibrils, globosebasidiospores, and pileal squamules made up of pseudoparenchymatous epithelium with encrusted walls. Molecular phylogenetic treeswere inferred based on nucleotide sequences of the internal transcribed spacer (ITS1-5.8S-ITS2) region and 28S nuclear ribosomalDNA. In phylogenetic analyses the genusHymenagaricussensu lato (s. l.) was inferred as a nonmonophyletic group and recoveredin two monophyletic clades, consisting of species of the generaXanthagaricusandHymenagaricussensu stricto (s. str.). On accountof the pileal squamule structure (pseudoparenchymatous epithelium) and yellowish basidiospores, and the phylogenetic position intheXanthagaricusclade the new speciesX. pakistanicusbelongs to the genusXanthagaricus . Morphoanatomical comparison with theknown species ofXanthagaricusis provided. We also made six new combinations inXanthagaricus :X. calicutensis ,X. epipastus ,X.ochraceoluteus ,X. rufomarginatus ,X. subaeruginosus , andX. taiwanensis . With this study the number of known species in the genusXanthagaricusincreases to 19.

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Berkeley MJ, Broome CE (1871). The Fungi of Ceylon. Bot J Linn Soc 11: 494-567.
Drummond AJ, Rambaut A (2007). BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 7: 214.
Gardes M, Bruns TD (1993). ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol Ecol 2: 113-118.
Ge ZW, Chen CM, Yang ZL (2008). A new species of the genus Hymenagaricus (Basidiomycota) from Taiwan and its phylogenetic position inferred from ITS and nLSU sequences. Cryptogamie Mycol 29: 259-265.
Gernhard T (2008). The conditioned reconstructed process. J Theor Biol 253: 769-778.
Heinemann P (1981). Hymenagaricus Heinem. gen. nov. (Agaricaceae). Bulletin du Jardin Botanique National de Belgique 51: 465-466.
Heinemann P, Little Flower Sr (1984). Hymenagaricus (Agaricaceae) de Kerala (Inde) et de Sri Lanka. Bulletin du Jardin Botanique National de Belgique 54: 151-182.
Hussain S, Afshan NS, Ahmad H, Khalid AN, Rehman AR (2017). Parasola malakandensis sp. nov. (Psathyrellaceae; Basidiomycota) from Malakand, Pakistan. Mycoscience 58: 69-76.
Kirk PM, Canon PF, Minter DW, Stalpers JA (2008). Ainsworth and Bisbys Dictionary of the Fungi. 10th ed. Wallingford, UK: CABI.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R et al. (2007). ClustalW and ClustalX version 2.0. Bioinformatics 23: 2947-2948.
Little Flower SR, Hosagoudar VB, Abraham TK (1997). Xanthagaricus , a new generic name in the family Agaricaceae. New Botanist 24: 93-100.
Miller MA, Pfeiffer W, Schwartz T (2010). Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, LA, USA, Nov 14, pp. 1-8.
Munsell AH (1975). Munsell Soil Color Charts. Baltimore, MD, USA: Munsell Color Inc.
Pegler DN (1986). Agaric flora of Sri Lanka (Kew Bulletin Additional Series XII). Kew, UK: Royal Botanical Gardens.
Petch T (1907). Revisions of Ceylon fungi. In Willis JC, editor. Annals of the Royal Botanic Gardens, Peradeniya, Vol. 4. Colombo, Sri Lanka: H. C. Cottle Government Printer Ceylon, pp. 21-68.
Petch T (1917). Revisions of Ceylon fungi. In Willis JC, editor. Annals of the Royal Botanic Gardens, Peradeniya, Vol. 6. Colombo, Sri Lanka: H. C. Cottle Government Printer Ceylon, pp. 307-355.
Rambaut A, Suchard MA, Xie D, Drummond AJ (2014). Tracer v1.6. Computer program and documentation distributed by the authors. http://beast.bio.ed.ac.uk/Tracer. Accessed 25 January 2017.
Reid DA, Eicker A (1995). The genus Hymenagaricus Heinem. in South Africa. S Afr J Bot 61: 293-297.
Reid DA, Eicker A (1998). South African Fungi 8. Three new species of Hymenagaricus from South Africa, with a revised key to South African taxa. S Afr J Bot 64: 356-360.
Reid DA, Eicker A (1999). South African fungi 10: new species, new record and new observations. Mycotaxon 73: 169-197.
Simmons MP, Norton AP (2014). Divergent maximum-likelihood- branch-support values for polytomies. Mol Phylogenet Evol 73: 87-96.
Saccardo PA (1887). Sylloge Hymenomycetum, Vol. 1. Agaricineae. Sylloge Fungorum. 5: 1-1146.
Swofford DL (2004). PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sunderland, MA, USA: Sinauer Associates.
Tibuhwa DD, Mwanga Z (2014). Morphology and molecular taxonomy of Hymenagaricus mlimaniensis species nov: a new Basidiomycota mushroom from Mlimani main campus, Tanzania. Journal of Yeast and Fungal Research 5: 96-102.
Vellinga EC, Sysouphanthong P, Hyde KD (2011). The family Agaricaceae: phylogenies and two new white-spored genera. Mycologia 103: 494-509.
Vellinga EC (2004). Genera in the family Agaricaceae: evidence from nrITS and nrLSU sequences. Mycol Res 108: 354-377.
White TJ, Bruns T, Lee S, Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocols: a Guide to Methods and Applications. New York, NY, USA: Academic Press, pp. 315-322.