Morphology and genetic affinities of a novel Chattonella isolate (Raphidophyceae) isolated from Iran’s south coast (Oman Sea)

The morphology and genetic affinity of a novel raphidophyte belonging to the genus Chattonella Biecheler is described for the first time from the Oman Sea along the south-east coast of Iran. While morphologically very similar to Chattonella subsalsa Biecheler, the Iranian isolates contain a distinct red eyespot. A comparison of LSU-rDNA and rDNA-ITS show that the Iranian isolate is genetically distinct from other Chattonella subsalsa strains isolated from across a wide global range and indicates that the Iranian isolates represent a distinct species related to Chattonella subsalsa.

Morphology and genetic affinities of a novel Chattonella isolate (Raphidophyceae) isolated from Iran’s south coast (Oman Sea)

The morphology and genetic affinity of a novel raphidophyte belonging to the genus Chattonella Biecheler is described for the first time from the Oman Sea along the south-east coast of Iran. While morphologically very similar to Chattonella subsalsa Biecheler, the Iranian isolates contain a distinct red eyespot. A comparison of LSU-rDNA and rDNA-ITS show that the Iranian isolate is genetically distinct from other Chattonella subsalsa strains isolated from across a wide global range and indicates that the Iranian isolates represent a distinct species related to Chattonella subsalsa.

___

Adachi M, Sako Y, Ishida Y (1994). Restriction fragment length polymorphism of ribosomal DNA internal transcribed spacer and 5.8s regions in Japanese Alexandrium species (Dinophyceae). J Phycol 30: 857–863.

Aizdaicher NA (1993). Morphological investigations of flagellate alga Heterosigma akashiwo in a culture. Russ Mar Biol 18: 163–166. Attaran-Fariman G, Javid P (2013). The phylogeny of Heterocapsa sp. (Dinophyceae) isolated from the south coast of Iran during a Cochlodinium polykrikoides bloom. Turk J Bot 37: 778-783.

Band-Schmidt CJ, Morquecho L, Hernandez-Becerril DU (2004). Raphidophyceans on the coasts of Mexico. Hydrobiologia 515: 79–

Bolch CJS, Blackburn SI, Hallegraeff GM, Vaillancour RE (1998). Genetic variation among different global populations of Gymnodinium catenatum revealed by RAPD-PCR. In: Reguera B, Blanco J, Fernandez ML, Wyatt T, editors. Harmful Microalgae. Paris: Xunta de Galicia and UNESCO, pp. 283– 2

Bolch CJS, Campbell CN (2004). Morphology and phylogenetic affinities of Thecadinium foveolatum sp. nov. (Dinophyceae: Thecadiniaceae), a new marine benthic dinoflagellate from the west of Scotland. Euro J Phycol 39: 351–360.

Bowers HA, Tomas C, Tengs T, Kempton JW, Lewitus AJ, Oldach DW (2006). Raphidophyceae [Chadefaud ex Silva] systematics and rapid identification: sequence analyses and real-time PCR assays. J Phycol 42: 1333–48.

Chang MH, Mcveagh M, Gall M, Smith P (2012). Chattonella globosa is a member of Dictyophyceae: reassignment to Vicicitus gen. nov., based on molecular phylogeny, pigment composition, morphology and life history. Phycologia 51(4): 403–420.

Chopin T, Bird CJ, CA M, Osborne JA, Patwary MUR, Floch JY (1996). A molecular investigation of polymorphism in the North Atlantic red alga Chondrus crispus (Gigartinales). Phycol Res 44: 69–80.

Connell LB (2002). Rapid identification of marine algae (Raphidophyceae) using three-primer PCR amplification of nuclear internal transcribed spacer (ITS) regions from fresh and archived material. Phycologia 41: 15–21.

Connell LB (2000). Nuclear ITS region of the alga Heterosigma akashiwo (Chromophyta: Raphidophyceae) is identical in isolates from Atlantic and Pacific basins. Mar Biol 136: 953– 9

Cucchiari E, Pistocchi R, Pezzolesi L, Penna A, Battocchi C, Cerino F, Totti C (2010). Resting cysts of Fibrocapsa japonica (Raphidophyceae) from coastal sediments of the northern Adriatic Sea (Mediterranean Sea). Harmful Algae 10: 81–87. Daugbjerg N, Hansen G, Larsen J, Moestrup Ø (2000). Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmored dinoflagellates. Phycologia 39: 302–317.

Demura M, Noël M, Kasai F, Watanabe M, Kawachi M (2009). Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristic and genetic diversity. Phycologia 48: 518–535.

Dündar E, Akçiçek E, Dirmenci T, Akgün Ş (2013). Phylogenetic analysis of the genus Stachys sect. Eriostomum (Lamiaceae) in Turkey based on nuclear ribosomal ITS sequences. Turk J Bot 37: 14–23.

Edvardsen B, Eikrem W, Shalchian-Tabrizi K, Riisberg I, Johnsen G, Naustvo I, Throndsen J (2007). Verrucophora farcimen gen. et sp. nov. (Dictyochophyceae Heterokonta)—a bloom-forming ichthyotoxic flagellate from the Skagerrak, Norway. J Phycol 43: 1054–1070.

Hall TA (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/ NT. Nucleic Acids Res Sympo Series 41: 95–98.

Kooistra W, de Boer MK, Vrieling EG, Connell LB, Gieskes WWC (2001). Variation along ITS markers across strains of Fibrocapsa japonica (Raphidophyceae) suggests hybridisation events and recent range expansion. J Sea Res 46: 213–222.

Landsberg JH (2002). The effects of harmful algal blooms on aquatic organisms. Rev Fisher Sci 10: 113–390.

Latif S, Ayub Z, Siddiqui G (2013). Seasonal variability of phytoplankton in a coastal lagoon and adjacent open sea in Pakistan. Turk J Bot 37: 389–410.

Marchant H, Thomas D (1983). Polylysine as an adhesive for the attachment of nanoplankton to substrates for electron microscopy. J Microsc 131: 127–129.

Marshall JA, Nichols PD, Hallegraeff GM (2002). Chemotaxonomic survey of sterols and fatty acids in six marine raphidophyte algae. J Appl Phycol 14: 255–265.

Matsubara T, Nagasoe S, Yamasaki Y, Shikata T, Shimasaki Y, Oshima Y, Honjo T (2007). Effects of temperature, salinity, and irradiance on the growth of the dinoflagellate Akashiwo sanguinea. J Exp Mar Biol Ecol 342: 226–230.

Nabavi SB (2010). HABs in Iran south coasts. Website http:/ marinenews.ir/

Oda T, Nakamura A, Shikayama M, Kawano I, Muramatsu T, Ishimatsu A (1997). Generation of reactive oxygen species by raphidophycean phytoplankton. Biosci Biotech Bioch 61: 1658–1662.

Peperzak L (2001). Raphidophytes. In: Garces E, Zingone A, Montresor M, Reguera B, BD, editors. LifeHAB, Life Histories of Microalgal Species Causing Harmful Blooms. Spain: Cmima-Csic, pp. 80–84.

Potter D, Saunders GW, Andersen RA (1997). Phylogenetic relationships of the Raphidophyceae and Xanthophyceae as inferred from nucleotide sequences of the 18S ribosomal RNA gene. Am J Bot 84: 966–972.

Sako Y, Otaka I, Uchida A (2000). The harmful algae Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) are phylogenetically the same species. In: Ninth International Conference on Harmful Algal Blooms, 7–11 February 2000, Hobart, Tasmania, Australia.

Shen M,  Xu J,  Chan AK,  Au D (2011). Susceptibility of fish to Chattonella marina is determined by its tolerance to hypoxia. Mar Pollut Bull 63: 189–94.

Turkish Journal of Botany-Cover
  • ISSN: 1300-008X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

The role of Mn-SOD and Fe-SOD genes in the response to low temperature in chs mutants of Arabidopsis

Zahra GHARARI, Ramazanali KHAVARI NEJAD, Reza SHEKASTE BAND, Farzane NAJAFI, Mohammad NABIUNI, Saeed IRIAN

Morphology and genetic affinities of a novel Chattonella isolate (Raphidophyceae) isolated from Iran’s south coast (Oman Sea)

Gilan ATTARAN-FARIMAN, Christopher John Stanley BOLCH

Salicylic-acid-induced recovery ability in salt-stressed Hordeum vulgare plants

Hadi PIRASTEH-ANOSHEH, Gholamhassan RANJBAR, Yahya EMAM, Muhammad ASHRAF

The revision of the section Alopecuroidei of the genus Astragalus (Fabaceae) in Turkey

Hasan AKAN, Zeki AYTAÇ

Genome size and phylogenetic relationships between the Tunisian species of the genus Calligonum (Polygonaceae)

Hassen GOUJA, Alfredo GARCÍA-FERNÁNDEZ, Teresa GARNATJE, Aly RAIES, Mohamed NEFFATI

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

Tijana BANJANAC, Branislav SILER, Marijana SKORIC, Nabil GHALAWENJI

Modulation of osmotic adjustment and enzymatic antioxidant profiling in Apera intermedia exposed to salt stress

Evren YILDIZTUGAY, Ceyda ÖZFİDAN KONAKÇI, Mustafa KÜÇÜKÖDÜK, Yağmur DURAN

Does gypsum influence seed germination?

Eva M. CAÑADAS, Miguel BALLESTEROS, Francisco VALLE, Juan LORITE

Expression of SOD gene and evaluating its role in stress tolerance in NaCl and PEG stressed Lycopersicum esculentum

Semra AYDIN, İlker BÜYÜK, Emine Sümer ARAS

Phytoplankton functional groups provide a quality assessment method by the Q assemblage index in Lake Mogan (Turkey)

Ayşe Nilsun DEMİR, Özden FAKIOĞLU, Berrin DURAL