Tijana BANJANAC, Branislav SILER, Marijana SKORIC, Nabil GHALAWENJI

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

Most of the species from the genus Centaurium Hill readily hybridize between themselves in natural habitats. Artificial interspecific hybrid plants were obtained under in vitro conditions using reciprocal crosses between 4 species: Centaurium erythraea, C. littorale, C. maritimum, and C. pulchellum. Among 76 crossing experiments performed, 3 types of crosses produced seeds and gave 20 interspecific hybrid plants. Nuclear DNA quantification, fingerprinting of molecular markers, and determination of variation in main secondary metabolite content were employed to confirm their hybrid nature. All 3 approaches were capable of detecting hybrid plants. RAPD molecular markers proved to be the most reliable, always placing the hybrids between their parents in dendrograms, while secoiridoid glycoside content also showed intermediate profiles in the hybrid plants in relation to the parents. Hence, each of the techniques employed can be recommended for elucidating the mechanisms of interspecific hybridization in natural populations of Centaurium species in further studies. This could help to comprehend their breeding system and recognize interspecific hybridization as one of the crucial steps in polyploid formation and speciation in this genus.

Anahtar Kelimeler:

Key words: Centaurium, interspecific hybridization, molecular markers, ploidy level, secoiridoid glycosides

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

Keywords:

interspecific hybridization, molecular markers, ploidy level, secoiridoid glycosides, Centaurium,

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The authors are grateful to Milutin S. Djuričković for language improvement. This work is dedicated to our dear colleague Prof Dragoljub Grubišić, whom we wish to remember with affection. This work was supported by the Ministry of Education, Science, and Technological Development of Serbia (grant no.173024).