Semra HASANÇEBİ, Neslihan Turgut Kara Özgür ÇAKIR, Şule ARI, Özgür ÇAKIR

Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae)

Efficient micropropagation and root culture protocols were developed for the endemic Astragalus chrysochlorus Boiss. & Kotschy. A high frequency of shoot formation (100%) and maximum multiplication (13 shoots per hypocotyl explant) were achieved on Murashige and Skoog (MS) media supplemented with 0.5 mg/L trans-Zeatin riboside (ZR). By using single regenerated hypocotyl explants, rooting was achieved at a rate of 93% on MS medium containing 2% sucrose without growth regulators. High frequency callus initiation and growth were achieved when single hypocotyl explants were inoculated on MS medium supplemented with 0.5 mg/L 2,4-Dichlorophenoxyacetic acid. Plant regeneration through indirect organogenesis was achieved on MS medium supplemented with 0.5 mg/L ZR. Root cultures were successfully established in liquid MS medium containing 0.5 mg/L a-Naphthaleneacetic acid. The optimised in vitro propagation, callus culture, and root culture protocols offer the possibility to use cell/root culture techniques for vegetative propagation and secondary metabolism studies on Astragalus L. species.

Anahtar Kelimeler:

Key words: Astragalus, endemic, regeneration, organogenesis, plant conservation

Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae)

Keywords:

Key words: Astragalus, endemic, regeneration, organogenesis, plant conservation,

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