Investigation of the effects of overexpression of Novel_105 miRNA in contrasting potato cultivars during separate and combined drought and heat stresses

Investigation of the effects of overexpression of Novel_105 miRNA in contrasting potato cultivars during separate and combined drought and heat stresses

Potato (Solanum tuberosum L.) growth, development and production are sensitive to abiotic stress conditions, yet studies about investigation of stress response of potato plants in regulation of gene expression level are limited. The aim of this study was to identify the role of newly identified Novel_105 miRNA in contrasting potato cultivars (tolerant Unica and sensitive Russet Burbank) in response to heat, drought and combined heat and drought stresses using transgenic approach. Transgenic plants of Unica and Russet Burbank were generated with overexpression of pre-miRNA of Novel_105 and both wild type and transgenic T0 lines were subjected to stress. Physiological parameters (gaseous exchange, leaf temperature, chlorophyll content and relative water content) were observed along with biochemical parameters including proline, malondialdehyde and hydrogen peroxide. The overexpression of Novel_105 improved physiobiochemical functions of the both cultivars under single or combined stress conditions. The increase in Novel_105 miRNA expression was observed along with a decreased expression in its predicted target E3 ubiquitin-protein ligase XBAT35, that is responsible for cell death in potato. This is a preliminary study for the development of abiotic stress resilient potato cultivars proving the overexpression of Novel_105 miRNA increased abiotic stress tolerance presumably by repressing the expression of XBAT35 responsible from promoting cell death.

___

  • Aksoy E, Demirel U, Öztürk ZN, Çalişkan S, Çalişkan ME (2015). Recent advances in potato genomics, transcriptomics, and transgenics under drought and heat stresses: A review. Turkish Journal of Botany 39: 920–940.
  • Ashraf M, Foolad MR (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59: 206–216.
  • Ashraf M, Harris P (2013). Photosynthesis under stressful environments: an overview. Photosynthetica 51: 163–190.
  • Asthir B (2015). Mechanisms of heat tolerance in crop plants. Biologia Plantarum 59: 620−628.
  • Barozai MYK, Wahid HA (2012). In silico identification and characterization of cumulative abiotic stress responding genes in potato (Solanum tuberosum L.). Pakistan Journal of Botany 44: 57−69.
  • Carvalho SD, Saraiva R, Maia TM, Abreu IA, Duque P (2012). XBAT35, a novel Arabidopsis RING E3 ligase exhibiting dual targeting of its splice isoforms, is involved in ethylene-mediated regulation of apical hook curvature. Molecular Plant 5: 1295–1309.
  • Chaudhry UK, Gökçe ZN, Gökçe AF (2020). Effects of salinity and drought stresses on the physio-morphological attributes of onion cultivars at bulbification stage. International Journal of Agriculture and Biology 24: 1681–1691.
  • Choudhury FK, Rivero RM, Blumwald E, Mittler R (2017) Reactive oxygen species, abiotic stress and stress combination. Plant Journal 90: 856−867.
  • Dahal K, Li XQ, Tai H, Creelman A, Bizimungu B (2019). Improving potato stress tolerance and tuber yield under a climate change scenario–a current overview. Frontiers in Plant Science 10: 563.
  • Deblonde PMK, Ledent JF (2001). Effects of moderate drought conditions on green leaf number, stem height, leaf length and tuber yield of potato cultivars. European Journal of Agronomy 14: 31−41.
  • Delazari FT, Assis IR, Cabrera DFV, Ferreira MG et al. (2018). Morphophysiological characteristics by sweet potato cultivars as function of irrigation depth. Anais da Academia Brasileira de Ciências 90: 3541−3549.
  • Demirel U, Çalışkan S, Yavuz C, Tindaş I, Polgar Z et al. (2017). Assessment of morphophysiological traits for selection of heattolerant potato genotypes. Turkish Journal of Agriculture and Forestry 41: 218−232.
  • Demirel U, Morris WL, Ducreux LJ, Yavuz C, Asim A et al. (2020). Physiological biochemical and transcriptional responses to single and combined abiotic stress in stress-tolerant and stress-sensitive potato genotypes. Frontiers in Plant Science 11: 169.
  • Din M, Barozai MYK (2014). Profiling microRNAs and their targets in an important fleshy fruit: tomato (Solanum lycopersicum). Gene 535: 198–203.
  • Din M, Barozai MYK, Baloch IA (2014). Identification and functional analysis of new conserved microRNAs and their targets in potato (Solanum tuberosum L.). Turkish Journal of Botany 38: 1199–1213.
  • Djami-Tchatchou AT, Sanan-Mishra N, Ntushelo K, Dubery IA (2017). Functional roles of micrornas in agronomically important plants— potential as targets for crop improvement and protection. Frontiers in Plant Science 22: 378.
  • Efeoglu B, Terzioglu S (2009). Photosynthetic responses of two wheat varieties to high temperature. Eurasian Journal of Biological and Chemical Sciences 3: 97–106.
  • Hancock RD, Morris WL, Ducreux LJ, Morris JA, Usman M et al.(2014). Physiological, biochemical and molecular responses of the potato (Solanum tuberosum L.) plant to moderately elevated temperature. Plant, Cell & Environment 37: 439–450.
  • Handayani T, Watanabe K (2020). The combination of drought and heat stress has a greater effect on potato plants than single stresses. Plant, Soil and Environment 66: 175−182.
  • Heath RL, Packer L (1968). Photooxidation in isolated chloroplasts. Archives of Biochemistry and Biophysics 125: 189–198.
  • Hwang EW, Shin SJ, Yu BK, Byun MO, Kwon HB (2011). miR171 family members are involved in drought response in Solanum tuberosum. Journal of Plant Biology 54: 43–48.
  • Jensen CR (1981). Influence of soil water stress on wilting and water relations of differently osmotically adjusted wheat plants. New Phytologist 89: 15−24.
  • Jones-Rhoades MW, Bartel DP, Bartel B (2006). MicroRNAs and their regulatory roles in plants. Annual Review of Plant Biology 57: 19– 53.
  • Kaplan E (2017). Patateste kuraklık ve yüksek sıcaklık streslerine tepkide rol oynayan miRNA’ların yeni nesil dizileme yöntemi ile belirlenmesi. MSc, Niğde Ömer Halisdemir Üniversitesi, Niğde, Turkey.
  • Katsoulas N, Elvanidi A, Ferentinos KP, Kacira M, Bartzanas T et al. (2016). Crop reflectance monitoring as a tool for water stress detection in greenhouses: A review. Biosystems Engineering 151: 374−398.
  • Koroban NV, Kudryavtseva AV, Krasnov GS, Sadritdinova AF, Fedorova MS et al. (2016). The role of microRNA in abiotic stress response in plants. Molecular Biology 50: 337–343.
  • Li Q, Serio RJ, Schofield A, Liu H, Rasmussen SR et al. (2020). Arabidopsis RING‐type E3 ubiquitin ligase XBAT35.2 promotes proteasome‐dependent degradation of ACD11 to attenuate abiotic stress tolerance. Plant Journal 104: 1712−1723.
  • Li S, Zhang N, Zhu X, Ma R, Yang J et al. (2020). Enhanced drought tolerance with artificial microRNA‐mediated StProDH1 gene silencing in potato. Crop Science 60: 1462–1471.
  • Li X, Ramírez DA, Qinc J, Dormateya R, Bia Z et al. (2019) Water restriction scenarios and their effects on traits in potato with different degrees of drought tolerance. Scientia Horticulturae 256: 108525.
  • Liu H, Ravichandran S, Teh OK, McVey S, Lilley C et al. (2017). The RING-type E3 ligase XBAT35.2 is involved in cell death induction and pathogen response. Plant Physiology 175: 1469−1483.
  • Loreto F, Velikova V (2001). Isoprene produced by leaves protects the photosynthetic apparatus against ozone damage, quenches ozone products, and reduces lipid peroxidation of cellular membranes. Plant Physiology 127: 1781−1787.
  • Mattioli R, Costantino P, Trovato M (2009). Proline accumulation in plants. Plant Signaling & Behavior 4: 1016−1018.
  • Megha S, Basu U, Kav NN (2018). Regulation of low temperature stress in plants by microRNAs. Plant, Cell & Environment 41:1-15.
  • Monneveux P, Ramírez DA, Pino MT (2013). Drought tolerance in potato (S. tuberosum L.): Can we learn from drought tolerance research in cereals. Plant Science 205: 76−86.
  • Morales F, Ancín M, Fakhet D, González-Torralba J, Gámez AL et al. (2020). Photosynthetic metabolism under stressful growth conditions as a bases for crop breeding and yield improvement. Plants 9: 88.
  • Naz N, Durrani F, Shah Z, Khan NA, Ullah I (2018). Influence of heat stress on growth and physiological activities of potato (Solanum tuberosum L.). Phyton-International Journal of Experimental Botany 87: 225–230.
  • Obidiegwu JE, Bryan GJ, Jones HG, Prashar A (2015). Coping with drought: stress and adaptive responses in potato and perspectives for improvement. Frontiers in Plant Science 6: 542.
  • Pieczynski M, Marczewski W, Hennig J, Dolata J, Bielewicz D et al. (2013). Down‐regulation of CBP 80 gene expression as a strategy to engineer a drought‐tolerant potato. Plant Biotechnology Journal 11: 459–469.
  • Pradhan GP, Prasad PV, Fritz AK, Kirkham MB, Gill BS (2012). Effects of drought and high temperature stress on synthetic hexaploid wheat. Functional Plant Biology 39: 190–198.
  • Rajwanshi R, Chakraborty S, Jayanandi K, Deb B, Lightfoot DA (2014). Orthologous plant microRNAs: microregulators with great potential for improving stress tolerance in plants. Theoretical and Applied Genetics 127: 2525–2543.
  • Rizhsky L, Liang H, Shuman J, Shulaev V, Davletova S et al.(2004). When defense pathways collide: the response of Arabidopsis to a combination of drought and heat stress. Plant Physiology 134: 1683–1696.
  • Rolando JL, Ramírez DA, Yactayo W, Monneveux P, Quiroz R (2015). Leaf greenness as a drought tolerance related trait in potato (Solanum tuberosum L.). Environmental and Experimental Botany 110: 27−35.
  • Romero AP, Alarcón A, Valbuena RI, Galeano CH (2017). Physiological assessment of water stress in potato using spectral information. Frontiers in Plant Science 8: 1608.
  • Ruban AV (2016). Nonphotochemical chlorophyll fluorescence quenching: mechanism and effectiveness in protecting plants from photodamage. Plant Physiology 170: 1903–1916.
  • Saminathan T, Alvarado A, Lopez C, Shinde S, Gajanayake B et al. (2019). Elevated carbon dioxide and drought modulate physiology and storage-root development in sweet potato by regulating microRNAs. Functional & Integrative Genomics 19: 171−190.
  • Sehgal A, Sita K, Kumar J, Kumar S, Singh S et al.(2017). Effects of drought, heat and their interaction on the growth, yield and photosynthetic function of lentil (Lensculinaris medikus) genotypes varying in heat and drought sensitivity. Frontiers in Plant Science 8: 1776.
  • Shriram V, Kumar V, Devarumath RM, Khare TS, Wani SH (2016). MicroRNAs as potential targets for abiotic stress tolerance in plants. Frontiers in Plant Science 7: 817.
  • Strech NA, Uhlmann LO, Zanon AJ, Bisognin DA (2012). Impact of elevated temperature scenarıos on potato leaf development. Engenharia Agrícola 32: 689−697.
  • Sunkar R, Li YF, Jagadeeswaran G (2012). Functions of microRNAs in plant stress responses. Trends in Plant Science 17: 196−203.
  • Urban J, Ingwers MW, McGuire MA, Teskey RO (2017). Increase in leaf temperature opens stomata and decouples net photosynthesis from stomatal conductance in Pinus taeda and Populus deltoides x nigra. Journal of Experimental Botany 68: 1757−1767.
  • Xiong L, Zhu JK (2002). Molecular and genetic aspect of plant responses to osmotic stresses. Plant, Cell & Environment 25: 131–139.
  • Yalçın M (2020). Investigation of function of novel_105 miRNA in potato cultivars using transgenic approach. MSc, Niğde Ömer Halisdemir Üniversitesi, Niğde, Turkey.
  • Yamaguchi-Shinozaki K, Shinozaki K (2006). Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annual Review of Plant Biology 57: 781–803.
  • Yang J, Zhang N, Ma C, Qu Y, Si H et al. (2013). Prediction and verification of microRNAs related to proline accumulation under drought stress in potato. Computational Biology and Chemistry 46: 48–54.
  • Yang J, Zhang N, Mi X, Wu L, Ma R et al. (2014). Identification of miR159s and their target genes and expression analysis under drought stress in potato. Computational Biology and Chemistry 53: 204–213.
  • Yu Y, Jia T, Chen X (2017). The ‘how’ and ‘where’ of plant microRNAs. New Phytologist 216: 1002–1017.
  • Zhang N, Yang J, Wang Z, Wen Y, Wang J et al. (2014). Identification of novel and conserved microRNAs related to drought stress in potato by deep sequencing. PloS One 9: 95489.
  • Zhang QF (2007). Strategies for developing green super rice. Proceedings of the National Academy of Sciences 104: 16402–16409.
  • Zheng LL, Qu LH (2015). Application of microRNA gene resources in the improvement of agronomic traits in rice. Plant Biotechnology Journal 13: 329–336.
Turkish Journal of Botany-Cover
  • ISSN: 1300-008X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Chaetocin enhances callus induction by decreasing the expression of major leaf polarity genes in Nicotiana tabacum

Ayyub EBRAHIMI, Nagihan ÖZSOY, Deniz GÜRLE, Baki YAMAN, Şule ARI

Investigation of the effects of overexpression of Novel_105 miRNA in contrasting potato cultivars during separate and combined drought and heat stresses

Melis YALÇIN, Zahide Neslihan ÖZTÜRK GÖKÇE

Application of structural, functional, fluorescent, and cytometric indicators for assessing physiological state of marine diatoms under different light growth conditions

Natalia SHOMAN, Ekaterina SOLOMONOVA, Arkadii AKIMOV

Integrating indicators of natural regeneration, enrichment planting, above-ground carbon stock, micro-climate and soil to asses vegetation succession in postmining reclamation in tropical forest

Trimanto, Lia HAPSARI, Sugeng BUDIHARTA

Ecological assessment of Burç Reservoir’s surface water (Turkey) using phytoplankton metrics and multivariate approach

Abuzer ÇELEKLİ, Gülümser ÖZPINAR

Metabolite profiling, distribution of secretory structures, and histochemistry in Curculigo orchioides Gaertn. and Curculigo latifolia Dryand. ex W.T.Aiton

Diah RATNADEWI, Abdul Halim UMAR, Mohamad RAFI, Yohana Caecilia SULISTYANINGSIH, Hamim HAMIM

Bioassessment of water quality of surface waters using diatom metrics

Abuzer ÇELEKLİ, Mehmet YAVUZATMACA, Ömer LEKESİZ

Using a supermatrix approach to explore phylogenetic relationships, divergence times, and historical biogeography of Saxifragales

Bryan T. DREW, Cara TARULLO, Jeffrey P. ROSE, Kenneth J. SYTSMA

Running sigmas analysis of sampled molecular paraphyly in Pottiaceae (Bryophyta)

Richard H. ZANDER

Taxonomic monograph of the tribe Nigelleae (Ranunculaceae): a group including ancient medicinal plants

Zübeyde UĞURLU AYDIN, Emel OYBAK DÖNMEZ, Ali A. DÖNMEZ