Júlia HALÁSZ, Attila HEGEDÜS, Bernadett SZIKRISZT, Sezai ERCİŞLİ, Emine ORHAN, Hakan Murat ÜNLÜ

7996

The S-genotyping of wild-grown apricots reveals only self-incompatible accessions in the Erzincan region of Turkey

The S-genotypes of 63 wild-growing Turkish apricots (Prunus armeniaca L.) were determined by PCR amplification of the S-RNase intron regions and SFB gene in order to characterise their sexual (in)compatibility phenotype. We determined the complete S-genotype of 63 wild-grown apricot accessions that originated in the Erzincan region. Ten previously described and 2 new S-alleles (provisionally labelled SX and SY) were identified in the genotypes. S2 was the most frequent S-allele in the tested germplasm (occurred in 19 accessions), followed by S8 (17), S19 (16), S3 (13), S12 (11), S6 (10), and S7 (10); while S9-, S11-, and S13-alleles were found in 8 accessions. A total of 36 different S-genotypes were assigned to the tested accessions. The SC-allele responsible for self-compatibility in apricot was not present, indicating that all accessions are self-incompatible. The analysis of S-allele frequencies allowed us to conclude the close relationship of wild-grown and cultivated apricots in Turkey and helped to raise hypotheses to explain high occurrences of S2- and S8-alleles.
Anahtar Kelimeler:

Key words: Crop evolution, PCR analysis, Prunus armeniaca, self-(in)compatibility, S-haplotype–specific F-box, S-ribonuclease

The S-genotyping of wild-grown apricots reveals only self-incompatible accessions in the Erzincan region of Turkey

The S-genotypes of 63 wild-growing Turkish apricots (Prunus armeniaca L.) were determined by PCR amplification of the S-RNase intron regions and SFB gene in order to characterise their sexual (in)compatibility phenotype. We determined the complete S-genotype of 63 wild-grown apricot accessions that originated in the Erzincan region. Ten previously described and 2 new S-alleles (provisionally labelled SX and SY) were identified in the genotypes. S2 was the most frequent S-allele in the tested germplasm (occurred in 19 accessions), followed by S8 (17), S19 (16), S3 (13), S12 (11), S6 (10), and S7 (10); while S9-, S11-, and S13-alleles were found in 8 accessions. A total of 36 different S-genotypes were assigned to the tested accessions. The SC-allele responsible for self-compatibility in apricot was not present, indicating that all accessions are self-incompatible. The analysis of S-allele frequencies allowed us to conclude the close relationship of wild-grown and cultivated apricots in Turkey and helped to raise hypotheses to explain high occurrences of S2- and S8-alleles.
Keywords:

Key words: Crop evolution, PCR analysis, Prunus armeniaca, self-(in)compatibility, S-haplotype–specific F-box, S-ribonuclease,

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  • Alburquerque N, Egea J, Pérez-Tornero O, Burgos L (2002). Genotyping apricot cultivars for self-(in)compatibility by means of RNases associated with S alleles. Plant Breed 121: 343–347.
  • Bourguiba H, Audergon JM, Krichen L, Trifi-Farah N, Mamouni A, Trabelsi S, D’Onofrio C, Asma BM, Santoni S, Khadari B (2012). Loss of genetic diversity as a signature of apricot domestication and diffusion into the Mediterranean Basin. BMC Plant Biol 12: 49.
  • Burgos L, Pérez-Tornero O, Ballester J, Olmos E (1998). Detection and inheritance of stylar ribonucleases associated with incompatibility alleles in apricot. Sex Plant Reprod 11: 153–158. de Cuyper B, Sonneveld T, Tobutt KR (2005). Determining selfincompatibility genotypes in Belgian wild cherries. Mol Ecol 14: 945–955. de Nettancourt D (2001). Incompatibility and Incongruity in Wild and Cultivated Plants. 2nd ed. New York: Springer-Verlag.
  • Egea J, Burgos L (1996). Detecting cross-incompatibility of three North-American apricot cultivars and establishing the first incompatibility group in apricot. J Amer Soc Hort Sci 121: 1002–1005.
  • Entani T, Iwano M, Shiba H, Che SF, Isogai A, Takayama S (2003). Comparative analysis of the self-incompatibility (S-) locus region of Prunus mume: identification of a pollen-expressed F-box gene with allelic-diversity. Genes Cells 8: 203–213.
  • Ercisli S (2004). A short review of the fruit germplasm resources of Turkey. Genet Resour Crop Ev 51: 419–435.
  • Halász J, Pedryc A, Ercisli S, Yilmaz KU, Hegedűs A (2012). Apricot self-incompatibility shows more complex picture than believed: an urge for harmonization. Acta Hort (ISHS) 966: 193–197.
  • Halász J, Pedryc A, Ercisli S, Yilmaz KU, Hegedűs A (2010). S-genotyping supports the genetic relationships between Turkish and Hungarian apricot germplasm. J Amer Soc Hort Sci 135: 410–417.
  • Halász J, Hegedűs A, Hermán R, Stefanovits-Bányai E, Pedryc A (2005). New self-incompatibility alleles in apricot (Prunus armeniaca L.) revealed by stylar ribonuclease assay and S-PCR analysis. Euphytica 145: 57–66.
  • Halász J, Pedryc A, Hegedűs A (2007). Origin and dissemination of the pollen-part mutated S C -haplotype that confers selfcompatibility in apricot (Prunus armeniaca). New Phytol 176: 793–803.
  • Hasanloo T, Sepehrifar R, Hajimehdipoor H (2011). Levels of phenolic compounds and their effects on antioxidant capacity of wild Vaccinium arctostaphylos L. (Qare-Qat) collected from different regions of Iran. Turk J Biol 35: 371–377.
  • Hegedűs A, Engel R, Abrankó L, Balogh E, Blázovics A, Hermán R, Halász J, Ercisli S, Pedryc A, Stefanovits-Bányai É (2010). Antioxidant and antiradical capacities in apricot (Prunus armeniaca L.) fruits: variations from genotypes, years and analytical methods. J Food Sci 75: C722–C730.
  • Hegedűs A, Taller D, Papp N, Szikriszt B, Ercisli S, Halász J, Stefanovits-Bányai É (2013). Fruit antioxidant capacity and self-incompatibility genotype of Ukrainian sweet cherry (Prunus avium L.) cultivars highlight their breeding prospects. Euphytica 191: 153–164.
  • Jahanban Esfahlan A, Jamei R (2012). Properties of biological activity of ten wild almond (Prunus amygdalus L.) species. Turk J Biol 36: 201–209.
  • Jie Q, Shupeng G, Jixiang Z, Manru G, Huairui S (2005). Identification of self-incompatibility genotypes of apricot (Prunus armeniaca L.) by S-allele-specific PCR analysis. Biotechnol Lett 27: 1205– 120
  • Lachkar A, Fattouch S, Ghazouani T, Halász J, Pedryc A, Hegedűs A, Mars M (2013). Identification of self-(in)compatibility S-alleles and new cross-incompatibility groups in Tunisian apricot (Prunus armeniaca L.) cultivars. J Hortic Sci Biotech 88: 497-501.
  • Leccese A, Bartolini S, Viti R (2012). From genotype to apricot fruit quality: the antioxidant properties contribution. Plant Foods Hum Nutr 67: 317–325.
  • McClure BA, Haring V, Ebert PR, Anderson MA, Simpson RJ, Sakiyama F, Clarke AE (1989). Style self-incompatibility gene products of Nicotiana alata are ribonucleases. Nature 342: 955–957.
  • Milatović D, Nikolić D, Rakonjac V, Fotirić-Akšić M (2010). Cross(in)compatibility in apricot (Prunus armeniaca L.). J Hortic Sci Biotech 85: 394–398.
  • Nyujtó F, Surányi D (1981). Kajszibarack. Mezőgazdasági Kiadó, Budapest.
  • Ortega E, Martínez-García P, Dicenta F, Egea J (2010). Disruption of endosperm development: an inbreeding effect in almond (Prunus dulcis). Sex Plant Reprod 23: 135–140.
  • Pedryc A, Ruthner S, Hermán R, Krska B, Hegedűs A, Halász J (2009). Genetic diversity of apricot revealed by a set of SSR markers from linkage group G1. Sci Hortic 121: 19–26.
  • Romero C, Vilanova S, Burgos L, Martinez-Calvo J, Vicente M, Llacer G, Badenes ML (2004). Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes. Plant Mol Biol 56: 145–157.
  • Schueler S, Tusch A, Scholz F (2006). Comparative analysis of the within-population genetic structure in wild cherry (Prunus avium L.) at the self-incompatibility locus and nuclear microsatellites. Mol Ecol 15: 3231−3243.
  • Sonneveld T, Tobutt KR, Robbins TP (2003). Allele-specific PCR detection of sweet cherry self-incompatibility (S) alleles S1 to S16 using consensus and allele-specific primers. Theor Appl Genet 107: 1059–1070.
  • Stone JL (2004). Sheltered load associated with S-alleles in Solanum carolinense. Heredity 92: 335–342.
  • Sutherland BG, Robbins TP, Tobutt KR (2004). Primers amplifying a range of Prunus S-alleles. Plant Breeding 123: 582–584.
  • Szabó Z, Nyéki J (1991). Blossoming, fructification and combination of apricot varieties. Acta Hort 293: 295–302.
  • Tao R, Watari A, Hanada T, Habu T, Yaegaki H, Yamaguchi M, Yamane H (2007). Self-compatible peach (Prunus persica) has mutant versions of the S haplotypes found in self-incompatible Prunus species. Plant Mol Biol 63: 109–123.
  • Vilanova S, Badenes ML, Burgos L, Martínez-Calvo J, Llácer G, Romero C (2006). Self-compatibility of two apricot selections is associated with two pollen-part mutations of different nature. Plant Physiol 142: 629–641.
  • Vilanova S, Romero C, Llácer G, Badenes ML, Burgos L (2005). Identification of self-(in)-compatibility alleles in apricot by PCR and sequence analysis. J Amer Soc Hort Sci 130: 893–898. Wright S (1939). The distribution of self-sterility alleles in populations. Genetics 24: 538–552.
  • Wu J, Gu C, Zhang SL, Zhang SJ, Wu HQ, Heng W (2009). Identification of S-haplotype-specific S-RNase and SFB alleles in native Chinese apricot (Prunus armeniaca L). J Hortic Sci Biotech 160: 241–248.
  • Yildiz H, Sengul M, Celik F, Hegedus A, Ercisli S, Tosun M (2010). Some phytochemical and antioxidant characteristics of wild and cultivated blackberry fruits (Rubus caucesicus). J Food Agric Environ 8: 156–159.
  • Zhang L, Chen X, Chen XL, Zhang C, Liu X, Ci ZJ, Zhang H, Wu C, Liu C (2008). Identification of self-incompatibility (S-) genotypes of Chinese apricot cultivars. Euphytica 160: 241– 2
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: 6
  • Yayıncı: TÜBİTAK
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