Tolga GÖKSAN, Ayşegül ZEKERİYAOĞLU, Alknur AK

The growth of Spirulina platensis in different culture system under greenhouse condition

Özet: Bu denemede, bir sera içinde bulunan farklı kültür düzeneklerinde üretilen Spirulina'mn büyüme karakteristiklerinin karşılaştırılması amaçlandı. Denemede, şeffaf bidonlar, polietilen torbalar ve raceway tipi havuzlar olmak üzere üç tip kültür düzeneği kullanıldı. Diğerlerine nazaran kültür sıcaklığının daha yüksek olması nedeniyle, bidon kültürlerinde daha yüksek hücre yoğunluğuna ulaşıldı. Deneme sonunda kuru ağırlık miktarı bidon kültürleri için 0.99 g L"1 iken diğerlerinde 0.5 g L"1 idi. Spesifik büyüme hızları bidon, torba ye havuz kültürleri için sırasıyla 0.32, 0.21 ve 0.20 gün-1 olarak bulundu. Deneme sonunda ölçülen protein miktarları ise bidon, torba ve havuz kültürleri için sırasıyla % 33.4, % 54.5 ve % 58.3 olarak bulundu. Bidon kültürlerindeki protein miktarlarının diğerlerine göre çok daha düşük bulunmasının nedeni, büyümenin daha hızlı olması ve kültürün durgunluk safhasında fazla kalması nedeniyle ortamdaki azotun tüketilmesi şeklinde yorumlandı. Sonuç olarak, küçük hacimli kültür sistemlerinin kullanılması, özellikle kış aylarında büyümeyi engelleyen en önemli faktör olan sıcaklığı arttıracaktır. Ayrıca, kültürlerde küçük hacim yanında kısa ışık yolu uzunluklarının da kullanımı daha yüksek bir üretim sağlayacaktır.

Spirulina platensis'in sera koşullarında farklı kültür sistemlerinde büyütülmesi

Abstract: We aimed in this experiment to compare the growth characteristics of Spirulina, which was cultivated in different culture vessels under greenhouse condition. Three types of culture vessels, i.e. transparent jars, polyethylene bags and raceway ponds, were used in the experiment. The jar cultures supported higher cell densities due to their higher culture temperature compared to the others. The dry weight amount in jar cultures was 0.99 g L"1 at the end of the experiment, while it was 0.5 g L"1 in the others. Specific growth rates were found to be 0.32, 0.21 and 0.20 day-1 in the jar, bag and pond cultures, respectively. The protein levels measured at the end of the experiment were 33.4, 54.5 and 58.3% for the jar, bag and pond cultures, respectively. The reason for the much lower protein amount in jar cultures was interpreted as the depletion of the nitrogen in the culture medium as a result of faster growth and the prolonged steady state. We concluded that the use of small volume cultures would increase the temperature faster, which is the main factor hindering growth especially in the winter period. Moreover, the use of short light-path lengths in addition to the smaller volumes in the cultures would support a higher productivity.

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