Variation of Callus Induction Through Anther Culture in Water Chestnut (Trapa sp.)

Effects of culture media and genotypes of water chestnut (Trapa sp.) on the frequency of callus induction from anther culture were investigated. Results revealed that the N6 media supplemented with 0.5 mg/l BA, 0.5 mg/l NAA and 0.1 mg/l GA3 were suitable for callus induction. Callus yielded poorly in MS-containing media compared to that of N6 media. No callus was induced on 2, 4-D containing media. Among the tested 18 genotypes, only 15 genotypes produced calli.

Variation of Callus Induction Through Anther Culture in Water Chestnut (Trapa sp.)

Effects of culture media and genotypes of water chestnut (Trapa sp.) on the frequency of callus induction from anther culture were investigated. Results revealed that the N6 media supplemented with 0.5 mg/l BA, 0.5 mg/l NAA and 0.1 mg/l GA3 were suitable for callus induction. Callus yielded poorly in MS-containing media compared to that of N6 media. No callus was induced on 2, 4-D containing media. Among the tested 18 genotypes, only 15 genotypes produced calli.

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  • Khan WA, Chughtai ID. Nutritive value of foodstuffs II - studies in ascorbic acid content of common vegetables. Pakistan J Sci Res 7: 50, 1955.
  • Bargale M, Sawarkar NJ, Sharma YK. Mineralogical composition of water chestnut as compared to sweet potato. Indian J Nutr Diet 24: 7882, 1987.
  • Daniel P, Vajravelu E, Thiyagaraj JG. Considerations on Trapa natans L. from Peninsular. India J Econ Tax Bot 4: 595-601, Kumar L, Singh SP, Pabuja SS. Studies on vegetative reproduction rate of water hyacinth and water chestnut. Indian J Agric Res 19: 58, 1985. (B) (C) (D) (E) (F)