Serdar ÜLKER, Arzu ÖZEL, Ahmet ÇOLAK, Şengül Alpay KARAOĞLU

Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil

This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and at 40 °C for 60 min. Ca2+ and Mn2+ enhanced lipase activity but it was determined that other metallic ions did not affect the enzyme activity. The Km and Vmax values of the crude enzyme for p-nitrophenyl butyrate hydrolysis were found to be 7.15 mM and 7.067 mM/min, respectively.

Anahtar Kelimeler:

Key words: Trichoderma harzianum, extracellular lipase, enzyme characterization

Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil

Keywords:

Key words: Trichoderma harzianum, extracellular lipase, enzyme characterization,

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