Induced karyomorphological variations in three phenodeviants of Capsicum annuun L.

EMS Capsicum annuum L.’de bir mutajen olarak karyomorfolojik mutantları izole etmek için kullanılmıştır. Capsicum annuum L. var Azad’ın tohumları ilk olarak distile suda ıslatıldı ve sonra 3, 5 ve 7 saat sure ile % 0,5 EMS çözeltisi ile muamele edilmiştir ve genetik ayrım yakından gözlemlenmiştir. Yapışık, köprü ve multivalen, ikincil bağlantılı, geri kalmış, erken gelişmiş hareketli gibi birçok kromozomal anomali üç muamelede de gözlenmiştir. Bu anomaliler doza bağımlı olarak sıklıkta artış göstermiştir. Morfolojik parametreler doz artışı ile azalan bir eğilim göstermiştir. Fakat 7 saatlik dozda morfolojik olarak 3 farklı bitki sadece diğer yakın bitkilerden değil aynı zamanda kontrol bitkilerinden de çeşitlilik gösteren bitki izole edilmiştir. Bunların sitolojik analizleri üç bitkinin hepsinde kromozomlar arası köprülerin baskınlığını ve artmış sıklıkta köprüler olduğunu göstermiştir. Bu yüzden bu iki faktörün birarada bu üç bitkinin farklılık göstermesinden sorumlu olduğu düşünülebilir.

Capsicum annuum L. bitkisinin üç farklı fenotipinde indüklenmiş karyomorfolojik varyasyonlar

EMS was used as a mutagen in Capsicum annuum L. to isolate karyomorphological mutants. Seeds of Capsicum annuum L. var Azad were first pre-soaked in distilled water and then treated with 0.5% solution of EMS for 3 durations, i.e. 3, 5, and 7 h, and genetic segregation was closely observed. Many chromosomal anomalies like stickiness, bridges, and multivalent, secondary associations, laggards, and precocious movement were observed after all 3 durations of treatment. These anomalies showed a dosedependent increase in frequency. The morphological parameters showed a decreasing trend along with the increasing dose. However, with the 7-h dose 3 morphologically distinct plants were isolated, which varied not only from other sib plants but also from control plants. Their cytological analysis revealed a predominance of bridges and increased frequency of bridges in all 3 plants. Therefore, it can be presumed that these 2 factors together may be responsible for creating deviations in these 3 plants.

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