Olgica STEFANOVİC, Ljiljana COMİC, Dragana STANOJEVIC, Slavica SOLUJIC-SUKDOLAK

Antibacterial activity of Aegopodium podagraria L. extracts and ınteraction between extracts and antibiotics

Aegopodium podagraria L. (Apiaceae) bitkisinin sıvı, etanol, ve etil asetat ekstresinin altı bakteriye karşı in vitro antibakteriyal etkileri çalışılmıştır. Antibakteriyal özellikleri disk diffüzyon ve tüp dilüsyon yöntemi ile çalışılmıştır. Minimal inhibisyon konsantrasyonlarına göre en yüksek aktivite (1.25-5 mg/ml) etanol ekstresinde gözlenmiştir. Denenen bakteriler içinde en hassas olanlar Enterobacter cloacae, Klebsiella pneumoniae ve Pseudomonas fluorescens bulunurken, Staphylococcus aureus bakterisi en dirençli bakteri olmuştur. Etanol ekstresi ticari antibiyotik ile mukayese edilmiştir. Sonuç olarak antibiyotik ekstre ilişkisi denenen insan patojen bakterilerine karşı etanol ekstresi/streptomisin ve etanol ekstresi/kloramfenikol sinerjistik, katkısal ve ilgisiz bulunmuştur. Sinerji Bacillus subtilis bakterisinde gözlenmiştir. Etanol ekstresinde konsantrasyon 1/4 MIC ve antibiyotik konsantrasyonunda 1/4 ve daha az bir MIC olarak hesaplanmıştır.

Aegopodium podagraria L. ekstresinin antibakteryal aktivitesi ve ekstre ile antibiyotik etkileşim

Antibacterial activities of aqueous, ethanol, and ethyl acetate extracts of Aegopodium podagraria L. (Apiaceae) were tested in vitro against 6 bacteria species. Antibacterial properties were determined by disk diffusion and tube dilution method. Based on the minimal inhibitory concentrations (MIC), the ethanol extract showed the highest activity (1.25-5 mg/ml). Among the tested bacteria, the most sensitive species were Enterobacter cloacae, Klebsiella pneumonia, and Pseudomonas fluorescens, while the most resistant was Staphylococcus aureus. Ethanol extract was chosen to investigate the effects of its combinations with commercial antibiotics by the checkerboard method. Results showed that the interactions between ethanol extract/streptomycin and ethanol extract/chloramphenicol were synergistic, additive, and indifferent against the tested human-pathogenic bacteria. Synergism was observed in relation to Bacillus subtilis. Synergy was confirmed at an ethanol extract concentration corresponding to 1/4 MIC and antibiotic concentrations corresponding to 1/4 MIC and lower.

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