Antibacterial activity of seed extracts of commerical and wild Lathyrus species

İki farklı Lathyrus türünün (Lathyrus ratan; ticari varyete ve L. aphaca; yabani varyete) tohumlarının butanolik ekstrelerinin in vitro antibakteriyal aktiviteleri çalışılmıştır. Bitki ve insan patojenlerine karşı antibakteriyal aktivite Disk Difizyon Yöntemi ile zon çapları ölçülerek yapılmıştır. Maksimum inhibisyon çapı L. ratan türünde gram pozitif bacteri olan-Staphylococcus aureus da gözlenmiştir. L. aphaca tohumları da inhibisyon göstermişlerdir, fakat Klebsiella pneumoniae bakterisinde aktiflik göstermemiştir. L. ratan ekstresinin L. aphaca dan daha aktif olduğu gözlenmiştir. Minimum inhibisyon konsantrasyonu (MIC) ve minimum bakteriyal konsantrasyon (MBC) değeri de hesaplanmıştır (μg/ml). En düşük değer Staphylococcus aureus [MIC-76,25 (L.a.) 78,5 (L.r.) MBC- 112,6 (L.a.) 98,35 (L.r.)] şeklinde gözlenmiştir. Tohum ekstresi tarafından en çok etkilenen S. aureus bakterisi olmuştur. Buna karşılık Bacillus subtilis bakterisi en az etkilenen bakteri olmuştur MIC [(98,50 (L.a.) 96,2 (L.r.)] ve MBC [169,50 (L.a.) 151,2 (L.r.)].

Ticari ve yabani tip Lathyrus türlerinin tohum ekstrelerinin antibakteriyal aktiviteleri

Butanolic extract of seeds from 2 different Lathyrus species (Lathyrus ratan [commercial variety] and L. aphaca [wild variety]), were investigated for their in vitro antibacterial activity. The antibacterial activity against plant and human pathogenic bacteria was evaluated on the basis of inhibition zones (IZs) measured by disc diffusion method. The maximum inhibition was shown by L. ratan against the gram-positive bacterium Staphylococcus aureus. L. aphaca seeds also showed inhibition but were inactive against Klebsiella pneumoniae. L. ratan extract was more active than L. aphaca. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for both the seeds (in μg/ml) were also determined. The lowest value was obtained for Staphylococcus aureus [MIC-76.25 (L.a.) 78.5 (L.r.) MBC- 112.6 (L.a.) 98.35 (L.r.)]; thus this bacterium was most inhibited by the seed extract, whereas Bacillus subtilis was least inhibited, as indicated by MIC [(98.50 (L.a.) 96.2 (L.r.)] and MBC [169.50 (L.a.) 151.2 (L.r.)] values.

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