İlhan GÜRBÜZ, Betül DEMİRCİ, Gerhard FRANZ

Comparison of the volatiles of Daphne pontica L. and D. oleoides Schreber subsp. oleoides isolated by hydro- and microdistillation methods

Aerial parts of Daphne pontica were collected from Ilgaz-Çankırı, and D. oleoides subsp. oleoides was collected from 2 different localities (Ayrancı-Karaman and Ilgaz-Çankırı) in Turkey. The samples were subjected to hydrodistillation and microdistillation. The resulting volatile samples were analyzed both by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), respectively. The main components for D. pontica were identified as hexahydrofarnesyl acetone (8.6%), carvacrol (8.5%), dihydroedulane II (4.7%), (E)-geranyl acetone (4.6%), and thymol (4.5%), while nonacosane (42.5% and 27.2%), hexadecanoic acid (24.4% and 20.0%), phytol (12.3%), and carvacrol (5.0%) were identified as the main components of D. oleoides subsp. oleoides obtained by hydrodistillation. Carvacrol (12.0%), thymol (7.7%), dihydroactinidiolide (7.2%), bicyclosesquiphellandrene (5.5%), and (Z)-3-hexenal (4.1%) were the major components in D. pontica, while carvacrol (27.2% and 25.4%), nonacosane (24.6%), (Z)-3-hexenal (18.5% and 2.5%), decane (7.4%), hexahydrofarnesyl acetone (7.4% and 2.2%), hexanal (6.6% and 1.5%), heptacosane (6.1%), nonanal (5.6% and 1.9%), thymol (5.1% and 2.3%), and phytol (5.0%) were identified in the D. oleoides subsp. oleoides isolated by microdistillation, respectively. In addition, the volatile components were evaluated for their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals using a bioautographic thin layer chromatography (TLC) method, and the samples showed activity comparable with that of the tested standards, vitamins C and E.

Anahtar Kelimeler:

Key words: Daphne sp., essential oil, volatiles, hydrodistillation, microdistillation, gas chromatography, gas chromatography-mass spectrometry, antioxidant activity

Comparison of the volatiles of Daphne pontica L. and D. oleoides Schreber subsp. oleoides isolated by hydro- and microdistillation methods

Keywords:

Key words: Daphne sp., essential oil, volatiles, hydrodistillation, microdistillation, gas chromatography, gas chromatography-mass spectrometry, antioxidant activity,

PDF

___

Tan K. Daphne L. In: Davis PH. ed. Flora of Turkey and the East Aegean Islands, Vol.7. Edinburgh University Press; 1982: pp. 521–526.
Baytop T. Therapy with Medicinal Plants in Turkey, in the Past and the Present. Nobel Tıp Basımevi. Istanbul; 1999.
Yeşilada E, Honda G, Sezik E et al. Traditional medicine in Turkey V. Folk medicine in the inner Taurus Mountains. J Ethnopharmacol 46: 133–152, 1995.
Fujita T, Sezik E, Tabata M et al. Traditional medicine in Turkey VII. Folk medicine in Middle and West Black Sea Regions. Econ Bot 49: 406–422, 1995.
Sezik E, Yeşilada E, Honda G et al. Traditional medicine in Turkey X. Folk medicine in Central Anatolia. J Ethnopharmacol 75: 95–115, 2001.
Yeşilada E, Sezik E, Honda G et al. Traditional medicine in Turkey IX. Folk medicine in North-West Anatolia. J Ethnopharmacol 64: 195–210, 1999.
Honda G, Yeşilada E, Tabata M et al. Traditional medicine in Turkey X. Folk medicine in Central Anatolia. J Ethnopharmacol 53: 75–87, 1996.
Özçelik H. Akseki yöresinde doğal olarak yetişen bazı faydalı bitkilerin yerel adları ve kullanılışları. Doğa: Turk Bot Derg 11: 316–321, 1987.
Ertuğ F. Bodrum yöresinde halk tıbbında yararlanılan bitkiler. In: Başer KHC, Kırımer N. eds. 14. Bitkisel İlaç Hammaddeleri Toplantısı Bildiri Kitabı. 2004: pp. 76–93.
Küpeli E, Orhan İ, Yeşilada E. Evaluation of some plants used in Turkish folk medicine for their anti-inflammatory and antinociceptive activities. Pharm Biol 45: 1–9, 2007.
Yeşilada E, Üstün O, Sezik E et al. Inhibitory effects of Turkish folk remedies on inflammatory cytokines: interleukin-1α, interleukin-1β and tumor necrosis factor α. J Ethnopharmacol 58: 59–73, 1997.
Yeşilada E, Taninaka H, Takaishi Y et al. In vitro inhibitory effects of Daphne oleoides ssp. oleoides on inflammatory cytokines and activity-guided isolation of active constituents. Cytokine 13: 359–364, 2001.
Briechle R, Dammertz W, Guth R et al. Bestimmung Ätherischer Öle in Drogen. GIT Fachz Lab 41: 749–753, 1997. McLafferty FW, Stauffer DB. The Wiley/NBS Registry of Mass Spectral Data. J. Wiley and Sons. New York; 1989.
Joulain D, König WA, Hochmuth DH. Terpenoids and Related Constituents of Essential Oils. Library of MassFinder 2.1. Hamburg; 2001.
Joulain D, König WA. The Atlas of Spectra Data of Sesquiterpene Hydrocarbons. EB-Verlag. Hamburg; 1998.
ESO 2000. The Complete Database of Essential Oils. Boelens Aroma Chemical Information Service. The Netherlands; 1999. Jennings WG, Shibamoto T. Quantitative Analysis of Flavor and Fragrance Volatiles by Glass Capillary GC. Academic Press. New York; 1980.
Kumarasamy Y, Fergusson ME, Nahar L et al. Bioactivity of moschamindole from Centaurea moschata. Pharm Biol 40: 307–310, 2002.
Sarker SD, Latif Z, Gray AI, eds. Natural Product Isolation in Natural Products Isolation. Humana Press. New Jersey; 2006. Demirci F, Demirci B, Gürbüz İ et al. Characterization and biological activity of Achillea teretifolia Willd. and A. nobilis subsp. neilreichii (Kerner) Formanek essential oils. Turk J Biol 33: 129–136, 2009.