Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on ITS-5.8S rDNA

In this study, 114 Rhizoctonia solani isolates were obtained from diseased common bean (Phaseolus vulgaris L.) plants from the Black Sea coastal region of Turkey. Genetic variation was determined among R. solani anastomosis group 4 (AG-4) subgroups (AG-4 HG-I and AG-4 HG-II). ITS-5.8 rDNA sequences of these isolates were aligned with other known R. solani sequences from GenBank, and distance and parsimony analysis were used to determine phylogenetic relationships. The R. solani AG-4 isolates were placed in 2 main lineages, corresponding to AG-4 HG-I and AG-4 HG-II. Based on phylogenetic analysis, genetic variations were shown in the AG-4 subgroups (especially AG-4 HG-I). This study represents the first reported use of rDNA-ITS sequence data to examine AG-4 HG-I and HG-II in P. vulgaris from Turkey. The results also suggest that the Giresun isolates are closely related to isolates obtained from Italy.

Anahtar Kelimeler:

Key words: rDNA-ITS phylogeny, Rhizoctonia solani, AG-4, Phaseolus vulgaris, common bean

Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on ITS-5.8S rDNA

Keywords:

Key words: rDNA-ITS phylogeny, Rhizoctonia solani, AG-4, Phaseolus vulgaris, common bean,

PDF

___

Sneh B, Jabaji-Hare S, Neate S et al. Rhizoctonia Species: Taxonomy, Molecular Biology, and Disease Control. Kluwer. Dordrecht; 1996.
Anderson NA. The genetics and pathology of Rhizoctonia solani. Annu Rev Phytopathol 20: 329–347, 1982.
Carling DE, Kuninaga S, Brainard KA. Hyphal anastomosis reactions, rDNA-internal transcribed spacer sequences, and virulence levels among subsets of Rhizoctonia solani anastomosis group-2 (AG-2) and AG-BI. Phytopathology 92: 43–50, 2002.
Sneh B, Burpee L, Ogoshi A. Identification of Rhizoctonia Species. American Phytopathological Society Press. St Paul, MN, USA; 1991.
Carling DE. Grouping in Rhizoctonia solani by the anastomosis reaction. In: Sneh B, Jabaji-Hare S, Neate S et al. eds. Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology, and Disease Control. Kluwer; 1996: pp. 35–47.
Qu P, Aratani A, Syoji T et al. Use of single-protoplast isolates in the study of the mating phenomena of Rhizoctonia solani (Thanatephorus cucumeris) AG-1 IC and IA. Mycoscience 49: 132–137, 2008.
Pannecoucque J, Van Beneden S, Höfte M. Characterization and pathogenicity of Rhizoctonia isolates associated with cauliflower in Belgium. Plant Pathol 57: 737–746, 2008.
Stevens Johnk J, Jones RK. Differentiation of three homogeneous groups of Rhizoctonia solani anastomosis group 4 by analysis of fatty acids. Phytopathology 91: 821–830, 2001. Kuninaga S, Yokosawa R. DNA base sequence homology in Rhizoctonia solani Kühn IV. Genetic relatedness within AG-4. Ann Phytopathol Soc Jpn 50: 322–330, 1984.
Toda T, Hyakumachi M, Suga H et al. Differentiation of Rhizoctonia AG-D isolates from turfgrass into subgroups I and II based on rDNA and RAPD analyses. Eur J Plant Pathol 105: 835–846, 1999.
Priyatmojo A, Yamauchi R, Kageyama K et al. Grouping of binucleate Rhizoctonia anastomosis group D (AG-D) isolates into subgroups I and II based on whole-cell fatty acid compositions. J Phytopathol 149: 421–426, 2001.
Çebi Kılıçoğlu M, Özkoç İ. Molecular characterization of Rhizoctonia solani AG4 using PCR-RFLP of the rDNA-ITS region. Turk J Biol 34: 261–269, 2010.
Pascual CB, Toda T, Raymondo AD et al. Characterization by conventional techniques and PCR of Rhizoctonia solani isolates causing banded leaf sheath blight in maize. Plant Pathol 49: 108–118, 2000.
White TJ, Bruns T, Lee S et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ et al. eds. PCR Protocols: A Guide to Methods and Applications. Academic Press; 1990: pp. 315–322.
Thompson JD, Gibson TJ, Plewniak F et al. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882, 1997.
Swofford DL. PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods), Version 4. Sinauer Associates, Sunderland, MA, USA, 2002.
Akaike H. A new look at the statistical model identification. IEEE Trans Automat Contr 19: 716–723, 1974.
Posada D, Crandall KA. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817–818, 1998.
Galindo JJ, Abawi GS, Thurston HD. Variability among isolates of Rhizoctonia solani associated with snap bean hypocotyls and soil in New York. Plant Dis 66: 390–394, 1982.
Galindo JJ, Abawi GS, Thurston HD et al. Source of inoculum and development of bean web blight in Costa Rica. Plant Dis 67: 1016–1021, 1983.
Galindo JJ, Abawi GS, Thurston HD et al. Effect of mulching on web blight of beans in Costa Rica. Phytopathology 73: 610– 615, 1983.