Murat AKKURT, Atilla ÇAKIR, Mina SHİDFAR, Filiz MUTAF, Gökhan SÖYLEMEZOĞLU

Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg × Sultani progeny

The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) × Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.

Anahtar Kelimeler:

Key words: Grapevine, marker-assisted selection, seedlessness

Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg × Sultani progeny

Keywords:

Key words: Grapevine, marker-assisted selection, seedlessness,

PDF

___

Roytchev V. Inheritance of grape seedlessness in seeded and seedless hybrid combinations of grape cultivars with complex genealogy. Am J Enol Viticult 49: 302–305, 1998.
This P, Lahogue F, Adam-Blondon AF et al. Towards markerassisted selection for seedlessness in grapevine. Acta Hortic 528: 221–229, 2000.
Cain DW, Emershad RL, Tarailo RE. In-ovulo embryo culture and seedling development of seeded and seedless grapes (Vitis vinifera L.). Vitis 22: 9–14, 1983.
Emershard RL, Ramming DW. In-ovulo embryo culture of Vitis vinifera L. cv ‘Thompson Seedless’. Am J Bot 71: 873–877, 19 Spiegel-Roy P, Sahar N, Baron J et al. In vitro culture and plant formation from grape cultivars with abortive ovules and seeds. J Am Soc Hortic Sci 110: 109–112, 1985.
Tangolar S, Eti S, Gök S et al. Obtaining plants from seedless x seedless grape crosses using embryo culture. Turk J Agric For 23: 935–942, 1999.
Mejia N, Hinrichsen P. A new, highly assertive SCAR marker potentially useful to assist selection for seedlessness in table grape breeding. Acta Hortic 603: 559–564, 2003.
Perl A, Sahar N, Eliassi R et al. Breeding of new seedless table grapes in Israel: conventional and biotechnological approach. Acta Hortic 603: 185–187, 2003.
Ledbetter CA, Burgos L. Inheritance of stenospermocarpic seedlessness in Vitis vinifera L. J Hered 85: 157–160, 1994.
Bouquet A, Danglot Y. Inheritance of seedlessness in grapevine (Vitis vinifera L.). Vitis 35: 35–42, 1996.
Lahogue F, This P, Bouquet A. Identification of a codominant SCAR marker linked to the seedlessness character in grapevine. Theor Appl Genet 97: 950–959, 1998.
Adam-Blondon AF, Lahogue-Esnault F, Bouquet A et al. Usefulness of two SCAR markers for marker-assisted selection of seedless grapevine cultivars. Vitis 40: 147–155, 2001.
Cabezas JA, Cervera MT, Ruiz-Garcia L et al. A genetic analysis of seed and berry weight in grapevine. Genome 49: 1572–1585, 200
Striem MJ, Ben-Hayyim G, Spiegel-Roy P. Identifying molecular genetic markers associated with seedlessness in grape. J Am Soc Hortic Sci 121: 758–763, 1996.
Yang KQ, Wang YJ, Zhang JJ et al. Analysis of restriction sites and Southern blotting of two molecular markers linked to grape seedless gene. Chin J Agr Biotechnol 3: 13–17, 2006.
Meija N, Gebauer M, Muñoz L et al. Identification of QTLs for seedlessness, berry size, and ripening date in a seedless x seedless table grape progeny. Am J Enol Viticult 58: 499–507, 200 Zhijian TL, Dhekney SA, Gray DJ. Molecular characterization of a SCAR marker purportedly linked to seedlessness in grapevine (Vitis). Mol Breeding 25: 637–644, 2010.
Mejía N, Soto B, Guerrero M et al. Molecular genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine. BMC Plant Biol 11: 57, 2011.
Doligez A, Bouquet A, Danglot Y et al. Genetic mapping of grapevine (Vitis vinifera L.) applied to the detection of QTLs for seedlessness and berry weight. Theor Appl Genet 105: 780– 795, 2002.
Costantini L, Battilana J, Lamaj F et al. Berry and phenologyrelated traits in grapevine (Vitis vinifera L.): from quantitative trait loci to underlying genes. BMC Plant Biol 8: 38, 2008.
Korpas A, Baranek M, Pidra M et al. Behaviour of two SCAR markers for seedlessness within Central European varieties of grapevine. Vitis 48: 33–42, 2009.
Fatahi R, Zamani Z, Ebadi A et al. The inheritance of seedless SCC8-SCAR and SSR loci alleles in progeny of ‘Muscat Hamburg’ x ‘Bidane Qermez’ grapes. Acta Hort 652: 329–335, 200