Biocontrol of Fusarium Wilt by Bacillus Pumilus, Pseudomonas Alcaligenes and Rhizobium Sp. on Lentil

The present study examined the effects of Bacillus pumilus, Pseudomonas alcaligenes, and Rhizobium sp. on wilt disease caused by Fusarium oxysporum f. sp. lentis and on the growth of lentil. Inoculation with F. oxysporum caused significant wilting, and reduced plant growth, the number of pods, and nodulation. Inoculation with B. pumilus together with P. alcaligenes caused a greater increase in plant growth, number of pods, nodulation, and root colonization by rhizobacteria, and also reduced Fusarium wilting to a greater degree than did individual inoculation. Use of Rhizobium sp. resulted in a greater increase in plant growth, number of pods, and nodulation, and reduced wilting more than B. pumilus did. Combined application of B. pumilus and P. alcaligenes with Rhizobium sp. resulted in the greatest increase in plant growth, number of pods, nodulation, and root colonization by rhizobacteria, and also reduced wilting in Fusarium-inoculated plants.

Biocontrol of Fusarium Wilt by Bacillus Pumilus, Pseudomonas Alcaligenes and Rhizobium Sp. on Lentil

The present study examined the effects of Bacillus pumilus, Pseudomonas alcaligenes, and Rhizobium sp. on wilt disease caused by Fusarium oxysporum f. sp. lentis and on the growth of lentil. Inoculation with F. oxysporum caused significant wilting, and reduced plant growth, the number of pods, and nodulation. Inoculation with B. pumilus together with P. alcaligenes caused a greater increase in plant growth, number of pods, nodulation, and root colonization by rhizobacteria, and also reduced Fusarium wilting to a greater degree than did individual inoculation. Use of Rhizobium sp. resulted in a greater increase in plant growth, number of pods, and nodulation, and reduced wilting more than B. pumilus did. Combined application of B. pumilus and P. alcaligenes with Rhizobium sp. resulted in the greatest increase in plant growth, number of pods, nodulation, and root colonization by rhizobacteria, and also reduced wilting in Fusarium-inoculated plants.

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  • Akhtar MS, Siddiqui ZA. Biocontrol of a root-rot disease complex of chickpea by Glomus intraradices, Rhizobium sp. and Pseudomonas straita. Crop Protect 27: 410-417, 2008.
  • Siddiqui ZA, Husain SI. Interaction of Meloidogyne incognita race 3, Macrophomina phaseolina and Bradyrhizobium sp. in the root-rot disease complex of chickpea, Cicer arietinum. Fundam Appl Nematol 15: 491-494, 1992.
  • Siddiqui ZA, Mahmood I. Role of bacteria in the management of plant parasitic nematodes. A Review. Bioresource Technol 69: 167-179, 1999.
  • Wilson M, Backman PA. Biological control of plant pathogens. In: Ruberson JR (ed): Handbook of Pest Management. Pp. 309- 335, Marcel-Dekker, Inc., New York, 1999.
  • Raupach GS, Kloepper JW. Mixture of plant growth-promoting rhizobacteria enhances biological control of multiple cucumber pathogens. Phytopathology 88: 1158-1164, 1998.
  • Riker AJ, Riker RS. Introduction to Research on Plant Diseases. John’s Swift Co. Inc., St. Louis, Chicago, New York, 1936. Siddiqui ZA, Baghel G, Akhtar MS. Biocontrol of Meloidogyne javanica by Rhizobium and plant growth promoting rhizobacteria on Lentil. World J Microbiol Biotechnol 23: 435- 441, 2007.
  • Sharma PD. Microbiology. Rastogi and company, Meerut, India, Pp. 359, 2001.
  • Dospekhov BA. Field Experimentation. Statistical Procedures. Mir Publishers, Moscow, 1984.
  • Weller DM. Biological control of soil borne plant pathogens in the rhizosphere with Bacteria. Ann Rev Phytopathol 26: 379- 407, 1988.
  • Whipps JM. Microbial interaction and biological in the rhizosphere. J Exp Bot 52: 487-511, 2001.
  • Egamberdieva D. Plant growth promoting properties of Rhizobacterail isolates from what and pea grown in loamy sand soil. Turk J Biol 32: 9-15, 2008.
  • Gholami A, Shahsavani S, Nezarat S. The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. Proceed World Acad Sci Eng Technol. 37: 19-24, 2009.
  • Siddiqui ZA, Mahmood I. Biological control of Heterodera cajani and Fusarium udum by Bacillus subtilis, Bradyrhizobium japonicum and Glomus fasciculatum on pigeonpea. Fundam Appl Nematol 18: 559-566, 1995.
  • Siddiqui ZA, Mahmood I. Biological control of Meloidogyne incognita race 3 and Macrophomina phaseolina by Paecilomyces lilacinus and Bacillus subtilis alone and in combination on chickpea. Fundam Appl Nematol 16: 215-218, 1993.
  • Lalande R, Bissonnette N, Coutlee D, Antoun H et al. Identification of rhizobacteria and determination of their plant- growth promoting potential, Plant and Soil 115: 7-11, 1989.
  • Suslow TV, Schroth MN. Rhizobacteria of sugar beets: Effects of seed application and root colonization on yield. Phytopathology 72: 199-206, 1982.
  • Gamliel A, Katan J. Suppression of major and minor pathogens by fluorescent pseudomonads in solarized and non-solarized soil. Phytopathology 83: 68-75, 1993.
  • Bapat S, Shah AK. Biological control of Fusarial wilts of pigeon pea by Bacillus brevis. Canadian J Microbiol 46: 125-131, 2000.
  • Chan YK, Wayne AM, Seifert KA et al. Characterization of antifungal soil bacterium and its antagonistic activities against Fusarium species. Canadian J Microbiol 49: 253-262, 2003.
  • Muhammad S, Amusa NA. In-vitro inhibition of growth of some seedling blight including pathogens by compost- inhabiting microbes. Afr J Biotechnol 2: 161-164, 2003.
  • Benhamou N, Kloepper JW, Quadt-Hallman A, Tuzun S et al. Induction of defence-related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria. Plant Physiol 112: 919-929, 1996.
  • Akhtar MS, Siddiqui ZA. Effects of Phosphate solubilizing microorganisms on the growth and root-rot disease complex of chickpea. Mycol Phytopathol 40: 246-254, 2006. Akhtar MS, Siddiqui ZA. Biocontrol of a chickpea root-rot disease complex with Glomus intraradices, Pseudomonas putida and Paenibacillus polymyxa. Austral Plant Pathol 36: 175-180, 2007.
  • Oostendrop M, Sikora RA. Utilization of antagonistic rhizobacteria as seed treatment for the biological control of Heterodera schachtii in sugarbeet. Rev Nematol 12: 77-83, 1989.
  • Siddiqui ZA, Sharma B, Siddiqui S et al. Evaluation of Bacillus and Pseudomonas isolates for the biocontrol of Meloidogyne incognita on tomato. Acta Phytopathol Entomol Hungar 42: 25- 34, 2007.
  • Wei G, Kloepper JW, Tuzun S et al. Induced systemic resistance to cucumber diseases and increased plant growth by plant growth promoting rhizobacteria under field conditions. Phytopathology 86: 221-224, 1996.
  • Haque SE, Ghaffar A. Use of rhizobia in the control of root-rot disease of sunflower, okra, soybean and mungbean. Phytopathol Z 138: 157-163, 1993.
  • Chakraborty U, Purkayastha RP. Role of Rhizobitoxine in protecting soybean roots from Macrophomina phaseolina infection. Canadian J Microbiol 30: 285-289, 1984.
  • Chakraborty U, Chakraborty BN. Interaction of Rhizobium leguminosarum and Fusarium solani f. sp. pisi in pea affecting disease development and phytoalexin production. Canadian J Bot 67: 1698-1701, 1989. Roslycky EB. Bacteriocin production in the rhizobia bacteria. Canadian J Microbiol 13: 431, 1967.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

The superiority of cv ‘rosea’ over cv ‘alba’ of periwinkle (Catharanthus roseus L.) in alkaloid production and other physiological attributes

Mohd IDREES, M. NAEEM, M. Masroor A. KHAN

Biocontrol of Fusarium Wilt by Bacillus Pumilus, Pseudomonas Alcaligenes and Rhizobium Sp. on Lentil

Mohd Sayeed AKHTAR*, Uzma SHAKEEL, Zaki Anwar SIDDIQUI

The effect of 4-chloroindole-3-acetic acid on some growth parameters in mung bean under cadmium stress

Mohammad MAZID, Barket ALI, Shamsul HAYAT, - *, Aqil AHMAD

Production of (S)-2'-fluorophenylethan-1-ol and (S)-3'- fluorophenylethan-1-ol by Alternaria alternata using ram horn peptone in microbial growth medium

Esabi Başaran KURBANOĞLU, - *, Namudar İzzet KURBANOĞLU, Kani ZILBEYAZ

Genetic relationships of the genera Onobrychis, Hedysarum, and Sartoria using seed storage proteins

Emine ARSLAN*, Kuddisi ERTUĞRUL

Stimulatory effects of ghrelin on spontaneous contractions in the rat myometrium

Alpaslan DAYANGAÇ, Selim KUTLU, Vahit KONAR

Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones

Manosh Kumar BISWAS, Uthpal Krishna ROY, Rafiul ISLAM, Monzur HOSSAIN

The superiority of cv ‘rosea’ over cv ‘alba’ of periwinkle (Catharanthus roseus L.) in alkaloid production and other physiological attributes

Mohd IDREES, M. NAEEM*, M. Masroor A. KHAN

The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips

Sanjay KUMAR, - *, Shashi Kiran ARYA, Bijoy Krishna ROY, Atul Kumar SINGH

Production of (S)-2'-fluorophenylethan-1-ol and (S)-3'- fluorophenylethan-1-ol by Alternaria alternata using ram horn peptone in microbial growth medium

Kani ZILBEYAZ, Namudar İzzet KURBANOĞLU, Esabi Başaran KURBANOĞLU